Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Abstract

We studied the functionality of the antioxidant system in laboratory rat cardiomyocytes and blood under psychoemotional stress. It was found that 40-day isolation and violation of diurnal cycle among the animals were accompanied by the intensification of lipid peroxidation process and marked with a reduced activity of antioxidant system enzymes, such as catalase and superoxide dismutase activity. The results suggested that psycho-emotional stress was accompanied by oxidative stress, causing a reduction in the intensity of energy metabolism in cardiomyocytes, which was further strengthened by the fact that the activity of the enzymes involved in ATP synthesis in mitochondria was reduced. Based on the results, we proposed that psychological stress is one of the factors contributing to the development of various cardiac diseases.

The Role of Microsomal Galactose-tissues Specific Lectins from Prostate in Gland Peroxidation

“…On the other hand, Ca 2+ ions had no effect on mitochondrial lectins [36]. We have ensured the clarity sub-cellular fractions considering the accuracy of the isolation method by the determination of the enzyme succinat dehydrogenase activity [22]. The afore-mentioned excludes possibility that microsomes and mitochondrial lectins are identical.…”

“…Microsomal fractions for lectin purification isolated from the human prostatic tissue as well as from the bovine liver according De-Robertis with differential centrifugation using sucrose density gradient. The purity of the microsomal fraction from the impurities of mitochondria was checked by measuring succinate dehydrogenase activity as the colored formazan product at the wavelength of 560 nm [22]. Microsomal fraction for the lectin isolation was treated with 0.5% detergent Triton-X-100 solution (40 mM KH 2 PO 4 , 0.15 M NaCl, pH 7.4 containing a cocktail of protease inhibitors).…”

Changes in activity of hippocampus creatine kinase under circadian rhythm disorders

“…During the experiments, the rats were given water and a standard laboratory chow ad libitum. The experiment was repeated four times (Kuchukashvili et al 2011.…”

“…Our previous experiments have detected a decrease in CK activity in brain cells and cardiomyocytes under chronic stress, accompanied by intensified lipid peroxidation and reduced activity of the antioxidant system. However, the nature of CK activity has not been specified Kuchukashvili et al 2011Kuchukashvili et al , 2012.…”

Redox Processes in Neurodegenerative Disease Involving Reactive Oxygen Species

“…The results suggest possible therapeutic use. Psycho-emotional stress in rats was accompanied by intensification of lipid peroxidation and reduced activity of AO enzymes, such as catalase and SOD [163]. The condition, involving OS, causes a reduction in the intensity of energy metabolism in cardiomyocytes.…”