Determination of hydroxyproline in plasma and tissue using electrospray mass spectrometry

Analysis of hydroxyproline isomers and hydroxylysine by reversed-phase HPLC and mass spectrometry

“…All four Hyp-isomers and Hyl are stable under these conditions; a significant degradation is not mentioned in the literature. Different methods based on gas or liquid chromatography have been described to analyze Hyp [10][11][12][13]. Most recent and sensitive techniques rely on precolumn derivatization, separation by reversed-phase HPLC followed by UV-or fluorescencedetection.…”

Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

“…8 Using the standard solution including leucine and isoleucine, the retention times were confirmed. As a result, Leu and Ile were eluted at around 3.5 min after sample injection in the chromatographic condition (data not shown), indicating that the peak of Hyp could be completely separated from those of Leu and Ile, and these two amino acids did not affect the determination of Hyp.…”

“…[3][4][5] To measure the concentrations of Hyp in biological samples or food, various methods have been developed, such as highperformance liquid chromatography (HPLC) with derivatization for fluorescence detection and high-performance liquid chromatography with tandem mass spectrometric detection (LC/ MS/MS). [6][7][8][9][10][11][12][13] We have developed an analytical method for the determination of urinary Hyp in rat. This method involves separating the Hyp in hydrolyzed urine by column-switching HPLC and detecting it with electrogenerated chemiluminescence (ECL) using tris(2,2-bipyridyl)ruthenium(II), utilizing an HPLC-ECL system developed in our laboratories.…”

Expression of transforming growth factor β1 gene, basic fibroblast growth factor gene and hydroxyproline in diabetes‐induced bladder dysfunction in a rat model

“…These added hydroxy groups help to stabilize the collagen fiber. 9 As for a reliable indicator of collagen catabolism, the content of hydroxyproline in each group was measured as a determination of bladder fibrosis. Our results showed that the content of hydroxyproline in the diabetic group was higher than that in the control group, which suggests that in the process of diabetes mellitus, hydroxylation of proline increases resulting in more collagen formation in the bladder wall.…”

“…It is located almost exclusively in collagen where it makes up approximately 10% of this protein. 9 Assessment of hydroxylation level may provide information regarding collagen accumulation in the extracellular matrix of the diabetic bladder.…”