Determination of human neutrophil antigen‐1, ‐3, ‐4 and ‐5 allele frequencies in <scp>E</scp>nglish <scp>C</scp>aucasoid blood donors using a multiplex fluorescent <scp>DNA</scp>‐based assay

Cardoso, S. P.; Chong, Winnie; Lucas, Geoff; Green, Anthony; Navarrete, Cristina

doi:10.1111/vox.12016

Cited by 27 publications

(52 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Genotype and allele frequencies of HNA‐3 in the populations of this study were compared with those published for other populations (Table ). The HNA‐3b/3b genotype frequency among the Zhuang population is 8.6%, which is similar to the frequencies reported in the Dong population of our study and to those reported for Chinese Han (He et al., ; Xia et al., ), Japanese (Matsuhashi et al., ), Thai (Changsri et al., ), German and Turkish populations (Hauck et al., ), but significantly higher than previously described in the African American (Huvard, Schmid, Stroncek, & Flegel, ), Brazilian (Lopes et al., ) and English Caucasian populations (Cardoso, Chong, Lucas, Green, & Navarrete, ) ( p < .05). The observed 7% frequency of HNA‐3b/3b in the samples from the Chinese Dong is lower than in the Zhejiang Han (He et al., ), but significantly higher than in African Americans (Huvard et al., ) ( p < .05).…”

Section: Resultssupporting

confidence: 91%

See 1 more Smart Citation

Genotype frequencies of human neutrophil antigen‐3 in the Chinese Zhuang and Dong populations

Liao

Jiao

et al. 2017

Int J Immunogenetics

View full text Add to dashboard Cite

Individuals with the human neutrophil antigen (HNA)-3b/3b type can produce HNA-3a antibodies, which have been reported to cause severe, sometimes fatal transfusion-related acute lung injury (TRALI). Our study aimed to determine the genotype frequency of HNA-3a/3b which will be helpful to estimate the potential risk for forming anti-HNA-3a, the clinically relevant antibody linked to TRALI in two different ethnic groups of southern China. Five hundred unrelated and healthy blood donors (284 male, 216 female; 300 Zhuangs, 200 Dongs) from the Guangxi Zhuang Autonomous Region were simultaneously typed for the HNA-3 allele using a polymerase chain reaction sequence-based typing (PCR-SBT) method. Genotype frequencies of HNA-3a/3a, HNA-3a/3b and HNA-3b/3b were 51.7%, 39.7% and 8.6% in the Zhuang population, and 44.0%, 49.0% and 7.0% in the Dong population, respectively. Homozygous HNA-3b/3b genotype frequency among the Zhuang population (8.6%) was significantly higher than previously reported in African Americans (0.4%), Brazilians (3.6%) and English Caucasians (2.9%) (p < .05). And the HNA-3b/3b genotype frequency among the Dong population was higher than African Americans (0.4%) (p < .05). This study showed Chinese Zhuang and Dong populations possessed a higher frequency of HNA-3b/3b genotype, suggesting that they may be at greater risk for developing anti-HNA-3a alloantibodies that may cause severe cases of TRALI. A molecular-based identification of the HNA-3b/3b genotype in all multiparous female blood donors was suggested to reduce the risk of TRALI following plasma and whole blood allogeneic transfusions.

show abstract

Section: Resultssupporting

confidence: 91%

“…() and also English Caucasian populations as revealed by Cardoso et al. (). Therefore, the Chinese Zhuang population may have a higher probability of producing anti‐HNA‐3a antibodies which is clinically associated with TRALI, compared with African American, Brazilian and English Caucasian populations.…”

Section: Discussionmentioning

confidence: 79%

Genotype frequencies of human neutrophil antigen‐3 in the Chinese Zhuang and Dong populations

Liao

Jiao

et al. 2017

Int J Immunogenetics

View full text Add to dashboard Cite

show abstract

“…On the other hand, HNA‐3 genotype distribution did not show any significant difference between Hong Kong Chinese and Germans ( P = 0·606), whereas a marginally significant difference between Chinese and British populations ( P = 0·049) was observed (Table ). The allele frequency of HNA‐3a was 71·0% in Hong Kong Chinese population, and it was quite similar to frequencies of 74·4% in German and 76·8% in British populations (Hauck et al, ; Cardoso et al, ). Surprisingly, Bowens et al .…”

Section: Discussionmentioning

confidence: 99%

A study of human neutrophil antigen genotype frequencies in Hong Kong

Tam

Tang

et al. 2017

Transfusion Medicine

View full text Add to dashboard Cite

This is the first study to define HNA genotype and allele frequencies using a validated modified in-house PCR-SSP method in the Hong Kong Chinese blood donor population. Our approach provides a cost-effective assay for conducting routine HNA typing and facilitates the incorporation of these assays into routine clinical service. Our results are comparable with those reported in the Guangzhou Chinese population, but the allele frequencies in our Hong Kong Chinese population are significantly different from the reported European frequencies, confirming that a geographical difference exists for HNA allele frequencies.

show abstract

“…The SLC44A2*2 allele frequencies vary among populations and range in the Han population from 38.2% (Nanning) to 26.2% (Guangzhou) . Although TRALI is much less frequently reported in China than in the United States, the susceptible allele SLC44A2*2 is more prevalent in any East Asian population compared to 17% in Caucasians and 7% in African Americans …”

mentioning

confidence: 99%

Genotype frequency of human neutrophil antigen‐3 polymorphisms in the Yi, Han, and Tibetan populations of China

et al. 2015

View full text Add to dashboard Cite

Background Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by PCR-SSP. We sequenced a SLC44A2 gene fragment and determined its allele frequencies in 3 ethnicities of China. Study design and methods Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of 2 SNPs; SLC44A2 haplotypes were constructed. Results In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66 and 0.69 in Yi, Han (Nanjing) and Tibetan, respectively (0.42, 0.34 and 0.31 for HNA-3b). Conclusions The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations.

show abstract

Determination of human neutrophil antigen‐1, ‐3, ‐4 and ‐5 allele frequencies in English Caucasoid blood donors using a multiplex fluorescent DNA‐based assay

Abstract: A multiplex Luminex bead assay for the simultaneous detection of HNA-1, HNA-3, HNA-4 and HNA-5 alleles is described that enables rapid typing of donors to support HNA alloimmunized patients who require HNA-compatible blood products.

Cited by 27 publications

References 27 publications

Genotype frequencies of human neutrophil antigen‐3 in the Chinese Zhuang and Dong populations

Genotype frequencies of human neutrophil antigen‐3 in the Chinese Zhuang and Dong populations

A study of human neutrophil antigen genotype frequencies in Hong Kong

Genotype frequency of human neutrophil antigen‐3 polymorphisms in the Yi, Han, and Tibetan populations of China

Contact Info

Product

Resources

About