Determination of homocysteine thiolactone in urine by field amplified sample injection and sweeping MEKC method with UV detection

Quantification of urinary S- and N-homocysteinylated protein and homocysteine-thiolactone in mice

“…Protein Slinked Hcy is assayed by capillary electrophoresis [30,31] or HPLC [32,33] following reduction of isolated proteins and derivatization of liberated Hcy. Hcy-thiolactone assays are based on a liquid or solid phase extraction procedures, followed by HPLC with fluorescence detection and quantification [27,34,35] or capillary electrophoresis with UV detection and quantification [36].…”

Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection

“…Micellar electrokinetic chromatography (MEKC) in the last few years become in many laboratories routine analytical tool for determination of biologically active compounds [21,[30][31][32]. Despite the huge analytical potential of electromigration techniques, namely quick separation, good resolution, high selectivity and little requirements as regards the size of the sample, they have some important limitations which arise especially when very complex biological samples should be analyzed.…”

“…One of them, so-called stacking enables preconcentration of neutral and ionic analytes. On-line stacking allows to determine very low concentration of analytes in biological matrices while high sensitivity and selectivity of method as well as efficient distribution of charged molecules puts it as one of the most interesting modern analytical techniques [18][19][20][21].…”

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

“…). This stacking method uses two different buffer systems . An alternative technique to FASI‐sweeping MEKC is the transient isotachophoresis (tITP) for on‐line sample preconcentration technique in high saline samples.…”