Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection

Furmaniak, Paulina; Kubalczyk, Paweł; Stachniuk, Justyna; Głowacki, Rafał

doi:10.1016/j.jchromb.2016.05.032

Cited by 8 publications

(7 citation statements)

References 39 publications

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“…A method for the determination of total 2‐mercaptoethanesulfonate in human plasma, based on high salt concentration stacking in MEKC and in‐capillary derivatization with 2‐chloro‐1‐methyllepidinium tetrafluoroborate was developed by Furmaniak et al. . 30 mM sodium phosphate buffer at pH 7 with 10 mM SDS and 10% ACN was used as the BGE and a pulse of 200 mM pH phosphate buffer at pH 7.8 was introduced after the sample for stacking of the micelles.…”

Section: Electrokinetic Chromatography Techniquesmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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Electrophoretic sample stacking comprises a group of capillary electrophoretic techniques where trace analytes from the sample are concentrated into a short zone (stack). This paper is a continuation of our previous reviews on the topic and brings a survey of more than 120 papers published approximately since the second quarter of 2016 till the first quarter of 2018. It is organized according to the particular stacking principles and includes chapters on concentration adjustment (Kohlrausch) stacking, on stacking techniques based on pH changes, on stacking in electrokinetic chromatography and on other stacking techniques. Where available, explicit information is given about the procedure, electrolyte(s) used, detector employed and sensitivity reached. Not reviewed are papers on transient isotachophoresis which are covered by another review in this issue.

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Section: Electrokinetic Chromatography Techniquesmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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“…There were 12 papers on in‐capillary derivatisation . The derivatisation was performed by adding the reagents to the separation buffer or by zone injections of reagents and sample.…”

Section: Derivatisation Modesmentioning

confidence: 99%

“…Furmaniak et al. demonstrated a MEKC‐UV approach for the analysis of 2‐mercaptoethanesulfonate via CMLT derivatisation of the thiol group . The three‐zone injection was sample, buffer, and CMLT and all of these zones were devoid of micelles.…”

Section: Derivatisation Modesmentioning

confidence: 99%

“…This is often the case for fluorescence detection where native fluorescence is rather uncommon and only the labelled species provide a strong signal. There were 24 articles on LIF , 16 on UV , 3 on electrochemical detection , and 4 on MS . In MS detection, derivatisation was used to improve the separation (see Section ) and to enable MS detection in favourable positive ion ESI mode .…”

Section: Derivatisation For Detectionmentioning

confidence: 99%

“…Common thiol‐reactive agents are iodoacetamides, maleimides, and benzylic halides. Głowacki and co‐workers used 2‐chloro‐1‐methyllepidinium tetrafluoroborate (CMLT) and 2‐chloro‐1‐methylquinolinium tetrafluoroborate (CMQT) to derivatise a drug and hydrogen sulfide . Both analytical workflows integrated stacking by sweeping to achieve sensitive analyte determinations.…”

Section: Derivatisation For Detectionmentioning

confidence: 99%

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Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Wuethrich

Quirino

2017

Electrophoresis

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Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

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In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Prapatpong

Nuchtavorn

Macka

et al. 2018

J of Separation Science

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In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20 kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7 min using 100 mM borate buffer (pH 10.0) containing 0.1% w/v of Brij®-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r > 0.98), precision (%RSDs < 3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25 μM, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

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Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection

Cited by 8 publications

References 39 publications

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

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