Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Abstract

Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection

“…Micellar electrokinetic chromatography (MEKC) in the last few years become in many laboratories routine analytical tool for determination of biologically active compounds [21,[30][31][32]. Despite the huge analytical potential of electromigration techniques, namely quick separation, good resolution, high selectivity and little requirements as regards the size of the sample, they have some important limitations which arise especially when very complex biological samples should be analyzed.…”

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

“…Autofluorescence is relatively uncommon and thus fluorescent derivatisation is generally applied to suit the analyte for detection. The derivatisation can be performed before injection and in-channel [50]. Fluorescence detection can also be extended to simultaneously detect multiple analytes.…”

Enantioseparation of d,l-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry—Fast and efficient tool for d- and l-2-hydroxyglutaracidurias diagnosis

“…Racemization of the resulted diastereomers can occur which can lead to an inaccurate result in the cases with a small difference in the concentration levels of the COAs enantiomers. Moreover, the methods employing chiral derivatization agents before the enantioseparation are time-consuming and the evaluation of recovery for the derivatization step is usually required [9].…”