Determination of genotoxicity of Fenaminosulf by Allium and Comet tests

Liman, Recep; Çiğerci, İbrahim Hakkı; Akyıl, Dilek; Eren, Yasin; Konuk, Muhsin

doi:10.1016/j.pestbp.2010.10.006

Cited by 53 publications

(42 citation statements)

References 33 publications

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“…The results of these assays are suitable for monitoring genotoxicity of environmental pollutants using plants. Liman et al (2011), using Fenaminosulf, fungicide and micro-biocide also noted a significant increase in DNA damage at a concentration of 25 ppm used for 24 h, 25 and 50 ppm for 96 h by Comet assay in A. cepa root tips. …”

Section: Resultsmentioning

confidence: 88%

“…However, the rate of changes in different phases was statistically non significant. Similarly, Liman et al (2011) investigated the genotoxic effects of fenaminosulf, fungicide and microbiocide, and found that rate of division of prophase cells increased at 24 h treatment at 12.5 and 50 ppm. Furthermore, they found that rate of division of cells decreased at metaphase, anaphase and telophase in all However, using 1 mg/L 2,4-D an increased metaphase or decreased anaphase and at 10 mg/L a decreased metaphase or increased anaphase activity was noted in root meristems of radish.…”

Section: Resultsmentioning

confidence: 99%

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Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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2,4-Dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator that is highly toxic to most broad leaved plants and relatively nontoxic to monocotyledonous plants; is frequently used as weed killer. The study aimed to investigate cytogenetic effects of different concentrations of 2,4-D (0.67, 1.34, 2.01, 2.68, 3.35 and 4.02 mg/L) on Allium cepa bulblets' root tips treated for 24 and 48 h. The results showed six types of structural aberrations: C-mitosis, stickiness, laggards, bridges, fragments and multipolarity that varied numerically compared to control. It significantly affected mitotic index (MI) at 24 and 48 h treatment. In the Allium test, MI increased significantly at three lower concentrations (0.67, 1.34, 2.01 mg/L) after treatment with 2,4-D for 24 h and decreased significantly at higher concentration. Whereas, 2,4-D treatment for 48 h increased MI at all concentrations with significantly decreased MI at the highest concentration. The experiment was extended using comet test that did not reveal significant difference among treatments except for application of 4.02 mg/L 2,4-D for 48 h; where cell damages were verified by comet test. Rest of the concentrations for any duration of time were not damaging and toxic to cells. The results showed, visible mitodepressive action of 4.02 mg/L 2,4-D when treated for 48 h that had tendency to become toxic if the roots had been in touch with 2,4-D for a longer time.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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“…Ames test, or so-called Salmonella/microsome test, is not only one of the most reliable short-term bacterial test systems but it is also cheap and gives results very rapidly. It has been widely used through out the world to detect mutagenic activity of pesticides via the detection of various types of gene mutations such as base-pair substitutions (TA100 and TA1535), frameshift mutations (TA98 and TA97) and transition or transversion events (TA102) (Konuk et al 2008;Liman et al 2010Liman et al , 2011. The results of the Ames test showed that 0.1, 1, 10 and 100 lg/plate doses of dicapthon were found to be weakly mutagenic in S. typhimurium TA98 without S9 mix.…”

Section: Discussionmentioning

confidence: 99%

Mutagenicity and genotoxicity of dicapthon insecticide

Liman

2014

Cytotechnology

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Mutagenic and genotoxic effects of dicapthon were investigated by using the bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9 mix), and chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests in human peripheral blood lymphocytes in vitro. Dicapthon was dissolved in dimethyl sulfoxide for all test systems. 0.1, 1, 10 and 100 lg/plate doses of dicapthon were found to be weakly mutagenic on S. typhimurium TA 98 without S9 mix. The human peripheral lymphocytes were treated with four experimental concentrations of dicapthon (25, 50, 100, and 200 lg/mL) for 24 and 48 h. Dicapthon increased the frequency of SCE only at the 100 lg/mL concentration for the 24 and 48 h applications. Dicapthon also induced abnormal cell frequency, CA/cell ratio and frequency of MN dose dependently for 24 and 48 h. Dicapthon showed a statistically significant cytotoxic effect by decreasing the mitotic index in all concentrations and a cytostatic effect by decreasing nuclear division index in 100 and 200 lg/mL concentrations for both treatment periods when compared with both untreated and solvent controls. These values decreased also in a dose dependent manner.

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“…Benzer sonuçların elde edildiği diğer çalışmalar sonucunda gözlenen bu etkinin CHFR (profaz/metafaz arası kontrol noktası) noktasından kaynaklandığı belirlenmiştir [27]. Scolnic ve Halazonetis [28] CHFR'yi, mitotik strese yanıt olarak, hücrelerin metafaz safhasına girmesini geciktiren bir denetim noktası olarak tanımlamışlardır.…”

Section: Tartişma Ve Sonuçunclassified

Anabolik Steroidlerin Genotoksik ve Sitotoksik Etkilerinin İnsan Periferal Lenfosit Kültüründe Mikronükleus Test Tekniğiyle Araştırılması

Çolak

Ayar

Uysal

2016

CSJ

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Özet. Spor tarihi boyunca genç yaştaki bir çok sporcunun doping amacıyla anabolik-androjenik steroidleri yoğun olarak kullandığı bilinmektedir. Bu çalışmayla, kullanımı yasaklanmış olsa da yapılan araştırmalarda illegal olarak halen sıkça kullanılmakta olan Nandrolone Decanoate (Deca-durabolin®) ve Stanozolol (Winstrol) doping maddelerinin olası genotoksik, mutajenik ve sitotoksik etkilerinin insan kan kültüründe in vitro mikronükleus testi ile belirlenmesi amaçlanmıştır. Bu amaçla doping maddelerinin farklı konsantrasyonları (1, 10, 25, 50, 75 ve 100 mM) hazırlanmış ve kan kültürüne uygulanmıştır. Genotoksik etki için mikronükleus frekansları, sitotoksik etki için ise Nükleer Bölünme İndeksi (NBİ) hesaplanmıştır. Elde edilen veriler, çözücü olarak kullanılan dimetil sülfoksit (DMSO) ile hazırlanan negatif kontrol grubuyla ve genotoksik etkisi çok iyi bilinen Etil metansülfonat (EMS) ile hazırlanmış pozitif kontrol grubuyla karşılaştırılmıştır. Bu sonuçlara göre; çalışmamızda kullandığımız doping maddelerinden Nandrolone Decanoate özellikle son iki konsantrasyonda (75 ve 100 mM) insan periferal lenfositlerinde mikronükleus frakansını istatistiksel olarak anlamlı (p< 0,05) bir şekilde artırmıştır. Ayrıca NBİ oranlarına bakıldığında da özellikle son iki konsantrasyonda kontrol grubuna göre önemli düşüşler tespit edilmiştir. Bununla birlikte diğer doping maddesi olan Stanozolol ise sadece son konsantrasyonda (100 mM) anlamlı artışlara neden olmuşken NBİ değerini etkilememiştir. Tüm elde ettiğimiz sonuçlardan her iki doping maddesinin de yüksek konsantrasyonlarda genotoksik ve sitotoksik etkiler yaratabileceği bu nedenle kullanımlarının tehlikeli olabileceği sonucuna ulaşılmıştır.Anahtar Kelimeler: Genotoksisite, Lenfosit kültürü, Mikronükleus, Nandrolone Decanoate, Stanozolol The Investigation of the Cytotoxic and Genotoxic Effcts of the Anabolic Steroids in Human Peripheral Lymphocytes Culture by Micronucleus TestAbstract. Throughout the sports history anabolic -androgenic steroids are known to be used intensively for the purpose of doping in athletes at very young age. In this study, the genotoxic, mutagenic and cytotoxic effects of possible doping substances, Nandrolone Decanoate (Deca-durabolin®) and stanozolol (Winstrol) that are currently being illegally widely used, though the use of them are prohibited, are used to determine in human blood culture by in vitro micronucleus test. For this purpose, different concentrations of doping substances (1, 10, 25, 50, 75 and 100 mM) were prepared and applied to the blood culture. For genotoxic effects micronucleus frequency and for to the cytotoxic effects Nuclear Fission Index (NBI) was calculated. The obtained data was compared to the negative control group that were prepeared with dimethyl sulfoxide (DMSO) as solvent and a well-known genotoxic effects of Ethyl methanesulfonate (EMS) as positive control group. Based on these results; in our study the doping substances Nandrolone decanoate caused a statistically significant (p< 0,05) increase in human peripheral lymph...

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Determination of genotoxicity of Fenaminosulf by Allium and Comet tests

Cited by 53 publications

References 33 publications

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

Mutagenicity and genotoxicity of dicapthon insecticide

Anabolik Steroidlerin Genotoksik ve Sitotoksik Etkilerinin İnsan Periferal Lenfosit Kültüründe Mikronükleus Test Tekniğiyle Araştırılması

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