Determination of genotoxic effects of copper sulphate and cobalt chloride in Allium cepa root cells by chromosome aberration and comet assays

Yıldız, Mustafa; Çiğerci, İbrahim Hakkı; Konuk, Muhsin; Fidan, Abdurrahman Fatih; Terzi, Hakan

doi:10.1016/j.chemosphere.2009.01.023

Cited by 161 publications

(49 citation statements)

References 30 publications

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“…Allium cepa assay is one of the best and extensively used system for the determination of cytotoxic and genotoxic impacts of pollutants, due to its sensitivity (Barberio et al 2011). Also, A. cepa showed a good correlation with the results from other established test systems using eukaryotic as well prokaryotic cells (Fiskesjö 1985;Yıldız et al 2009). …”

Section: Discussionmentioning

confidence: 98%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionmentioning

confidence: 98%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…In the meantime over 40 g/L concentration, roots became dark colored, thicker and gel like formations. The inhibition of root growth generally related to apical meristematic activity (Webster & Macleod, 1996), and to cell elongation during differentiation (Fusconi et al, 2006) and the appearance of stunted roots indicate the retardation of growth and cytotoxicity (Yıldız et al, 2009). As reported earlier, neoclerodane diterpenoids and flavonoids were isolated from Linaria saxatilis var.…”

Section: Resultsmentioning

confidence: 91%

“…In the determination of application doses, 2xEC50, EC50 and 0.5x EC5, positive (MMS, 10 ppm) and negative control group were used for 12, 24, 48, 72 and 96 h. Fixation and staining of the root tip cells were carried out as reported earlier (Yıldız et al, 2009;Liman et al, 2010). The MI and the frequencies of chromosomal aberrations (CA) were carried out according to Saxena et al (2005).…”

Section: Allium Cepa Anaphase-telophase Testmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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“…This test has been widely used in toxicity, mutagenicity and genotoxicity studies for diverse hazardous contaminants such as pesticides, dyes, food preservatives, and hydrocarbons (Fatima and Ahmad 2006;Mitteregger et al 2007;Feretti et al 2007;Türkoglu 2007;Leme and Marin-Morales 2008;Mustafa and Arikan 2008;Ashraf and Husain 2010), and is one of the most established test systems used to determine toxicity in several laboratories (Rank et al 2002;Chandra et al 2005;Yıldız et al 2009). This assay demonstrates alterations in all phases of the cell cycle, which are considered evidence for mutagenic effects induced by clastogenic or aneugenic agents (classified according to the type of alteration induced) (Vidakovié-cifrek et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Mutagenicity of two herbicides widely used on soybean crops by the Allium cepa test

et al. 2015

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This study evaluated the mutagenic effects of two herbicides: Clorimurom Nortox Ò and Imazaquim Ultra Nortox Ò widely used on soybean crops in Brazil. As a test system, Allium cepa assay was used, which analyzes the frequency of micronuclei (MN), chromosomal aberrations (CA) and the mitotic index (MI). Four concentrations of each herbicide (50, 75, 100 and 125 %) were tested in triplicate using distilled water (negative control) and methyl methanesulfonate (positive control) as controls. Three experimental repetitions were realized. Clorimurom Nortox Ò showed a significantly lower MI than the negative control for the concentrations of 75, 100 and 125 %, but the CA was significantly increased at all concentrations. There was no recovery for CA or MI. The 125 % concentration of Imazaquim Ultra Nortox Ò was cytotoxic and also exerted an effect on the other parameters. The concentration of 100 % showed a statistically increased MN and there was no recovery, while the 75 % concentration significantly affected CA, with recovery observed. The two herbicides showed mutagenic damage in Allium cepa cells, which implies a careful handling of these products, to minimize the risk of human and environmental contamination.

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Determination of genotoxic effects of copper sulphate and cobalt chloride in Allium cepa root cells by chromosome aberration and comet assays

Cited by 161 publications

References 30 publications

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Mutagenicity of two herbicides widely used on soybean crops by the Allium cepa test

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