Determination of free and total bisphenol A in the urine and feces of orally and subcutaneously dosed sheep by high-performance liquid chromatography with fluorescence detection

Šturm, Sabina; Škibin, Andrej; Pogačnik, M.; Cerkvenik-Flajs, Vesna

doi:10.1080/03601234.2020.1759329

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…This finding also demonstrated different MISPE BPA behaviours for clean-up of different matrix structures, e.g. for blood plasma [24] and urine [19] , where recovery rates were significantly higher (>76 and >60%, respectively) after washing the cartridge with MeCN, compared to, for example, the tissue of mussels (recovery of 47%) [18] . The MISPE clean-up simplification was also systematically described for ochratoxin A determination in pig muscle tissue, kidney and liver [20] .…”

Section: Resultsmentioning

confidence: 73%

“…muscle, kidney and liver). Within our recent research on BPA we were faced with relatively weak recovery rates for the analysis of marine mussel tissue [18] and sheep faeces [19] with a mean value of 47 and 52%, respectively. The clean-up of both analytical methods used was based on the method of Deceuninck et al [16] , by omitting the derivatization step and using HPLC-FLD instead of GC-MS/MS.…”

Section: Methods Detailsmentioning

confidence: 99%

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A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Cerkvenik-Flajs

Šturm

2021

MethodsX

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Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 µg/kg were in the range of 67‒86% and 3‒34%, respectively, while linearity in the matrix, represented by the r 2 , was ≥0.999. LOD values were 0.5‒1 µg/kg and LOQ values were 1 µg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision.

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Section: Resultsmentioning

confidence: 73%

Section: Methods Detailsmentioning

confidence: 99%

A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Cerkvenik-Flajs

Šturm

2021

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“…The only matrix that can be taken from a wild animal for toxicology studies without significantly interfering with its life are feces/guano samples. The utility of this matrix is also supported by the fact that previous studies have confirmed that both BPA and parabens are excreted from organisms through the digestive tract [43,44].…”

Section: Introductionmentioning

confidence: 80%

Evaluation of Parabens and Bisphenol A Concentration Levels in Wild Bat Guano Samples

Gonkowski

Martín

Aparicio

et al. 2023

IJERPH

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Parabens and bisphenol A are synthetic compounds found in many everyday objects, including bottles, food containers, personal care products, cosmetics and medicines. These substances may penetrate the environment and living organisms, on which they have a negative impact. Till now, numerous studies have described parabens and BPA in humans, but knowledge about terrestrial wild mammals’ exposure to these compounds is very limited. Therefore, during this study, the most common concentration levels of BPA and parabens were selected (such as methyl paraben—MeP, ethyl paraben—EtP, propyl paraben—PrP and butyl paraben—BuP) and analyzed in guano samples collected in summer (nursery) colonies of greater mouse-eared bats (Myotis myotis) using liquid chromatography with the tandem mass spectrometry (LC-MS-MS) method. MeP has been found in all guano samples and its median concentration levels amounted to 39.6 ng/g. Other parabens were present in smaller number of samples (from 5% for BuP to 62.5% for EtP) and in lower concentrations. Median concentration levels of these substances achieved 0.95 ng/g, 1.45 ng/g and 15.56 ng/g for EtP, PrP and BuP, respectively. BPA concentration levels did not exceed the method quantification limit (5 ng/g dw) in any sample. The present study has shown that wild bats are exposed to parabens and BPA, and guano samples are a suitable matrix for studies on wild animal exposure to these substances.

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“…Optimization of the enzymatic tissue hydrolysis revealed differences with the hydrolysis method described for other matrices e.g. blood plasma and milk (Šturm et al 2020a ) and urine and faeces (Šturm et al 2020b ) regarding sample aliquot, pH of buffer, time of incubation and volume of the enzyme used. A sample mass of 2.0 g was intermediate between e.g.…”

Section: Discussionmentioning

confidence: 99%

Bisphenol A in edible tissues of rams exposed to repeated low-level dietary dose by high-performance liquid chromatography with fluorescence detection

Cerkvenik-Flajs

Škibin

Švara

et al. 2022

Environ Sci Pollut Res

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The presented work deals with levels and distribution of bisphenol A (BPA) in the edible tissues of a large food-producing animal species. An experimental animal study included 14 young Istrian pramenka rams (Ovis aries), of which seven were exposed for 64 days to a low dietary dose of BPA at 25 µg/kg b.w./day, and seven served as a control group. Residue analysis of both aglycone and total BPA was performed in the muscle tissue, liver, kidney and fat tissue of the individual animals by means of enzymatic deconjugation (for total BPA), organic solvent extraction, molecularly imprinted polymer solid-phase extraction (MISPE) clean-up and high-performance liquid chromatography with fluorescence detection (HPLC-FLU). The analysis was optimized and validated for aglycone BPA in the fat tissue and for the total BPA in all tissues investigated. Edible tissues of the control group of rams generally remained BPA-free, while there were concentration differences between the control and treated groups for liver and kidney post last administration. The human health risk resulting from this study was assessed by the estimated dietary exposure in adults, which was < 0.1% related to the valid European Union Tolerable Daily Intake (TDI) value of 4 µg/kg b.w./day. However, it would be 58-fold higher than the newly proposed TDI value of 0.04 ng/kg b.w./day.

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Determination of free and total bisphenol A in the urine and feces of orally and subcutaneously dosed sheep by high-performance liquid chromatography with fluorescence detection

Cited by 9 publications

References 34 publications

A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Evaluation of Parabens and Bisphenol A Concentration Levels in Wild Bat Guano Samples

Bisphenol A in edible tissues of rams exposed to repeated low-level dietary dose by high-performance liquid chromatography with fluorescence detection

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