Determination of free and bound phenolics in northern highbush blueberries by a validated HPLC/QTOF methodology

Pico, Joana; Yan, Yifan; Gerbrandt, Eric M.; Castellarin, Simone D.

doi:10.1016/j.jfca.2022.104412

Cited by 18 publications

(9 citation statements)

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“…As shown in Table 4 and Figure 6 a, b, 11 flavonoid glycosides were identified by matching DAD and MS/MS features with those available in literature [ 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. Mainly catechin ( m / z 291.0869), cyanidin ( m / z 287.0551), pelargonidin ( m / z 271.0601), quercetin ( m / z 303.0505), and kaempferol ( m / z 287.0556) with various types and amounts of sugars were revealed ( Figure S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Mainly catechin ( m / z 291.0869), cyanidin ( m / z 287.0551), pelargonidin ( m / z 271.0601), quercetin ( m / z 303.0505), and kaempferol ( m / z 287.0556) with various types and amounts of sugars were revealed ( Figure S2 ). Figures S3–S7 illustrated the fragmentation patterns of 11 glycosylated flavonoids in the extract, and possible positions of different sugar groups on the rings were tentatively determined based on the literature [ 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. For example, the compound of peak 1 was identified as catechin-7-O-glucoside by the parent ion at m / z 453.1391 and fragment ions at m / z 291.0865 and m / z 139.0390, which were produced by the loss of glucose (162 Da) and the C-ring cleavage [ 47 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…Figures S3–S7 illustrated the fragmentation patterns of 11 glycosylated flavonoids in the extract, and possible positions of different sugar groups on the rings were tentatively determined based on the literature [ 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. For example, the compound of peak 1 was identified as catechin-7-O-glucoside by the parent ion at m / z 453.1391 and fragment ions at m / z 291.0865 and m / z 139.0390, which were produced by the loss of glucose (162 Da) and the C-ring cleavage [ 47 , 48 ]. The compound of peak 2 appeared at the parent ion at m / z 611.1608 of cyanidin-3,5-diglucoside, which produced the product ions of m / z 449.1075 and m / z 287.0544 by the loss of two molecules of glucose (162 Da), respectively [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

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Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity

et al. 2022

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The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization of UAATPE and UAAH processes was accomplished by single-factor experiments and response surface methodology. As a result, the flavonoid glycosides enriched in the top phase could achieve a maximum yield of 35.9 ± 1.1 mg/g by UAATPE and were completely hydrolyzed by UAAH deglycosylation. The flavonoid glycosides and their hydrolyzates were separated and characterized by high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Ultrasonic enhancement of the extraction and hydrolysis was explored by comparative study. Furthermore, the in vitro activity of the flavonoid glycosides and the aglycones were comprehensively evaluated by antioxidant activity assays, including ferric-reducing antioxidant power and scavenging DPPH, hydroxyl, and superoxide radicals. All of the IC50 values suggest that the antioxidant activity of flavonoid aglycones was stronger than that of their glucosides and even vitamin C, revealing that the deglycosylated flavonoids from MACF were the more powerful antioxidants. This study provided an effective and eco-friendly strategy for the extraction, separation, and purification of flavonoids from MACF, as well as for the development of the potential flavonoid antioxidants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity

et al. 2022

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“…However, there are also mixed juices with high antioxidant activity that are consumed in relatively large quantities [ 29 ]. The first group of juices includes pomegranate, cranberry, and blueberry, as well as other dark fruits, such as cherry and blackcurrant, which have higher levels of phenolic compounds (e.g., phenolic acids, flavonoids, anthocyanins, and tannins) [ 38 , 39 , 40 ] In the other category, research focused on orange, grape, and apple juices, which contain mainly hydroxycinnamic acids and vitamins [ 29 , 41 , 42 ].…”

Section: Fruits and Beverages As Functional Foodsmentioning

confidence: 99%

Cherries and Blueberries-Based Beverages: Functional Foods with Antidiabetic and Immune Booster Properties

et al. 2022

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Nowadays, it is largely accepted that the daily intake of fruits, vegetables, herbal products and derivatives is an added value in promoting human health, given their capacity to counteract oxidative stress markers and suppress uncontrolled pro-inflammatory responses. Given that, natural-based products seem to be a promising strategy to attenuate, or even mitigate, the development of chronic diseases, such as diabetes, and to boost the immune system. Among fruits, cherries and blueberries are nutrient-dense fruits that have been a target of many studies and interest given their richness in phenolic compounds and notable biological potential. In fact, research has already demonstrated that these fruits can be considered functional foods, and hence, their use in functional beverages, whose popularity is increasing worldwide, is not surprising and seem to be a promising and useful strategy. Therefore, the present review reinforces the idea that cherries and blueberries can be incorporated into new pharmaceutical products, smart foods, functional beverages, and nutraceuticals and be effective in preventing and/or treating diseases mediated by inflammatory mediators, reactive species, and free radicals.

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“…[ 18 , 19 ] and Artiplex halimus L. [ 20 ]. Syringetin-3- O -glucoside was also found in the skin of wine grapes such as Cabernet Sauvignon, Merlot, Syrah, and Marselan [ 21 ], needles of Larix decidua [ 22 ], blueberries [ 23 ], and fruits of Embelia ribes [ 24 ]. The presence of syringetin-3- O -glucoside and syringetin-3- O -acetylglucoside was confirmed for the first time in Cabernet Sauvignon grape and wines by Wang et al in 2003 [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

The Spectrum of Pharmacological Actions of Syringetin and Its Natural Derivatives—A Summary Review

Chmiel

Stompor-Gorący

2022

Nutrients

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Mono- and poly-O-methylated flavonols and their glycoside derivatives belong to the group of natural plant polyphenols with a wide spectrum of pharmacological activities. These compounds are known for their antioxidant, antimutagenic, hepatoprotective, antidiabetic, and antilipogenic properties. Additionally, they inhibit carcinogenesis and cancer development. Having in mind the multidirectional biological activity of methylated flavonols, we would like to support further study on their health-promoting activities; in this review we summarized the most recent reports on syringetin and some of its structural analogues: laricitrin, ayanin, and isorhamnetin. Natural sources and biological potential of these substances were described based on the latest research papers.

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Determination of free and bound phenolics in northern highbush blueberries by a validated HPLC/QTOF methodology

Cited by 18 publications

References 50 publications

Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity

Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity

Cherries and Blueberries-Based Beverages: Functional Foods with Antidiabetic and Immune Booster Properties

The Spectrum of Pharmacological Actions of Syringetin and Its Natural Derivatives—A Summary Review

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