Determination of folic acid in fortified wheat flours

Alaburda, Janete; Almeida, Adriana Palma de; Shundo, Luzia; Ruvieri, Valter; Sabino, Myrna

doi:10.1016/j.jfca.2007.12.002

Cited by 57 publications

(38 citation statements)

References 15 publications

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“…These values are within the range of 80 to 110% set by the AOAC [28]. Studies were found in the literature with similar percentages for the recovery of thiamin, of between 73 to 106% [21,23,24] and 81 to 103% for folic acid [25,27,29]. …”

Section: Recoverysupporting

confidence: 67%

“…The value found for thiamin was lower than the limits of detection found by Hucker et al [24], Presoto and AlmeidaMuradian [25], and San José Rodriguez [22], being 0.02 μg, 0.03 μg, and 0.06 μg, respectively. In relation to folic acid, values of 0.003 μg, 0.0013 μg, and 0.06 μg were found by Boen et al [26], Lebiedziñska et al [27], and Alaburda et al [28], respectively. The LOQ was 0.0193 μg for thiamin and 0.00934 μg for folic acid.…”

Section: Limits Of Detection and Quantificationmentioning

confidence: 87%

“…The coefficient of determination for thiamin and folic acid was 0.9998 and 0.9997, respectively. Studies analyzing these vitamins found coefficients of determination for thiamin of 0.9957 [21], 0.9967 [22], 0.9998 [23], and 0.9950 [24] and for folic acid of 0.9838 [22], 0.9997 [25], 0.9980 [26], and 0.9999 [27].…”

Section: Validation Of Analytical Methods For Folic Acid and Thiamin mentioning

confidence: 99%

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Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Silveira

Lúcia

Pirozi

et al. 2016

Acta Chromatographica

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Summary. This study aimed to optimize and validate methods for the analysis of thiamin and folic acid in fortified rice, pure and mixed to the milled rice (raw and cooked). The analysis was performed by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Different mobile phases were tested. Different ratios of organic modifier, pH ranges, triethylamine concentrations, and flow rates were used. For the validation, tests of recovery, repeatability, linearity, limit of detection (LOD), and limit of quantification (LOQ) were performed. The optimized methods showed good resolution of vitamins' peaks, excellent recovery (82.6 to 104%), repeatability with relative standard deviation of peak areas, and retention times less than 10% and high coefficients of determination (0.9998 for thiamin and 0.9997 for folic acid). The LOD and LOQ were 0.00193 μg and 0.0193 μg for thiamin and 0.000934 μg and 0.00934 μg for folic acid. The optimized methods demonstrated reliability and sensitivity in the detection and quantification of these vitamins in fortified rice, pure and mixed to milled rice (raw and cooked). Furthermore, the methods were performed in isocratic mode, with short run time (<13 min), reflecting positively on the economy of reagents and analysis times.

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Section: Recoverysupporting

confidence: 67%

Section: Limits Of Detection and Quantificationmentioning

confidence: 87%

Section: Validation Of Analytical Methods For Folic Acid and Thiamin mentioning

confidence: 99%

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Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Silveira

Lúcia

Pirozi

et al. 2016

Acta Chromatographica

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“…It has been found that 51 % of flour samples contain less than 150 µg of folic acid per 100 g (22) . Another study found that the variation of folic acid in flour was from 73 to 558 µg/100 g and of Fe was 3·8-8·7 mg/100 g (23) .…”

Section: Discussionmentioning

confidence: 99%

An evaluation of the effectiveness of the flour iron fortification programme in Brazil

Santos

Nilson

Verly

et al. 2014

Public Health Nutr.

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Objective: The present communication reports a strategy to calculate the intake of Fe based on data available for folic acid and evaluate the programme of flour fortification in Brazil. Design: Cross-sectional study conducted in Brazil during 2008 and 2009. A 2 d dietary record of individuals was used. The usual intake of folic acid by sex and age group was estimated using the National Cancer Institute method. The quantity of folic acid and Fe established by mandatory food fortification in Brazil was used, and based on that quantity we calculated the amount of flour consumed and the intake of Fe from fortification and Fe from food. Then, the absorption of each nutrient was calculated. Setting: Brazilian households (n 16 764). Subjects: Individuals (men and women, n 34 003, aged 10 to 60 + years) from a Brazilian nationwide survey. Results: Mean intake and absorption of Fe from fortification (electrolytic Fe) was low in men and women. Conclusions: The impact from the consumption of fortified products is small in relation to Fe intake in Brazil. The strategy proposed to estimate Fe intake from the fortification programme indicates that the amount of flour intake observed in Brazil does not justify the current ranges of mandatory flour fortification and the form of Fe that is mainly used (electrolytic Fe).

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“…In recent years, several methods for the determination of FA have been reported, including high-performance liquid chromatography (HPLC), [4][5][6][7][8][9] chemiluminescence (CL), [10][11][12] spectrophotometric, 13,14 electroanalytical, [15][16][17] and fluorometric methods. 18,19 However, HPLC technique has superiority in terms of separating and quantifying different forms of FA with the minimum interference from enzymes but it involves complicated system operation and maintenance, high consumption of samples and expensive reagents.…”

Section: 2mentioning

confidence: 99%

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

Alam¹,

Kamruzzaman²,

Lee³

et al. 2012

Bulletin of the Korean Chemical Society

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A simple, rapid and sensitive spectrofluorometric method was developed for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of enoxacin (ENX)-europium (Eu 3+) complex as a fluorescent probe. Fluorometric interaction between ENX-Eu 3+ complex and FA was studied using UVvisible and fluorescence spectroscopy. The quenched fluorescence intensity at an emission wavelength of 614 nm was proportional to the concentration of FA. Optimum conditions for the determination of FA were investigated. Under optimal conditions, the reduced fluorescence intensity at 614 nm was responded linearly with the concentration of FA. The linearity was maintained in the range of 1.25 × 10 −9 to 1.50 × 10 −7 M (R = 0.9986) with the limit of detection (3S b /m) (where S b is the standard deviation of blank and m is the slop of linear calibration curve) of 6.94 × 10 −10 M. The relative standard deviation (RSD) for 9 repeated measurements of 1.0 × 10 −9 M FA was 1.42%. This method was simple, cost effective, and relatively free of interference from coexisting substances. Successful determinations of FA in pharmaceutical formulation and biological samples with the developed method were demonstrated.

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Determination of folic acid in fortified wheat flours

Cited by 57 publications

References 15 publications

Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

An evaluation of the effectiveness of the flour iron fortification programme in Brazil

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

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