Determination of Extraction Process Conditions of Gambier Catechin (Uncaria Gambier Roxb) from Solok Bio Bio Lima Puluh Kota District – West Sumatera

Abstract

Abstract.Catechin content is the determinant key of quality in gambier trade. The required Catechin content of gambier extracts as a herbal medicinal ingridient is greater than 90%. Mostly, Local gambier that produced by community is not uniform and low quality, thus lowering the price in the export markets. The quality improvement of gambier can be done by extraction and purification processes. This study aims to determine the best extraction process of catechin from Gambier (Uncaria Roxb) which derived from Solok Bio Bio Lima Puluh Kota, West Sumatra. The research methodology includes pre purification: raw materials preparation, washing, filtration, extraction, drying and testing. Washing was done on 100 gr gambier with a variation of water at 500, 600, 700, and 800 ml, heating for an hour at a temperature of 70⁰C, screened, filtered, and allow to stand until a precipitate is formed, wash repeatedly, filtered, and dried. Further, extract with a solvent variation of : water, etyl acetate, heated at 70⁰C temperature for 1 hour, then filtered. Filtrate then thickened by using a Rotary evaporator, dried at 50⁰C temperature for 48 hours and analyzed. The results showed that the best conditions of the extraction process is by using a solvent etyl acetate, at a temperature of 70⁰C, grading 97.40% catechins.

Stain Fungi and Discoloration Control on Rubberwood (Hevea brasiliensis Muell. Arg.) by Vacuum-Pressure Treatment with Catechin from Gambir (Uncaria gambir Roxb.)

“…If any significant (p ≤ 0.05) features were in the data, the analysis was continued using Duncan's multiple range test. Rahman et al, 2018;Yeni et al, 2014), the use of ethyl acetate for gambir extraction is considered the optimal choice based on the variables of time and cost-effectiveness. Supporting the results reported by Nandika et al (2019), the physical characteristics of extracted catechin in this present study were in the form of a fine yellowish-white powder and were odorless, with a water content of 9.1%.…”

Bioactivities of catechin from gambir (Uncaria gambir Roxb.) against wood-decaying fungi

“…The difference in the yields could have been due to the temperature and time of extraction, size of the gambir blocks, as well as difference in the solvent used. Nevertheless, several publications (Yeni et al 2014;Rahman et al 2018;Failisnur et al 2018) stated that based on the considerations of time and cost effectivity, the use of ethyl acetate on gambir extraction is considered to be the best decision. At the initial stage of extraction, the gambir powder underwent a weight loss of 51.0%.…”