Determination of dioxin and dioxin-like compounds in sediments from UK estuaries using a bio-analytical approach: chemical-activated luciferase expression (CALUX) assay

Hurst, Mark R.; Balaam, Jan; Chan-Man, Yin L.; Thain, John E.; Thomas, Kevin V.

doi:10.1016/j.marpolbul.2004.04.012

Cited by 45 publications

(23 citation statements)

References 35 publications

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“…The present study is the first investigation using in vitro bioassay of dioxin-like compounds in sediments collected in this area. The results indicate that the dioxin-like activities in SCEs collected in the Tianjin area are similar to previous reports of sediments from UK estuaries (1.1-154 ng TCDD-EQ g À1 dw) (Hurst et al, 2004) and sediment from the Czech River Basin (0.782-22.3 ng TCDD-EQ g À1 dw) (Hilscherova et al, 2001). …”

Section: Ahr-mediated Activity Of Crude Extracts Of Sedimentssupporting

confidence: 89%

“…The most likely cause of the difference between the two methods of measuring AhR activity in the sediments is the presence of AhR-active compounds other than those measured in this study. Of those unknown compounds capable of producing dioxin-like responses in the H4IIE-luc assay that may be present in the samples the most likely are the PAHs (Hilscherova et al, 2001;Hurst et al, 2004). The compounds responsible for dioxin-like activity in F3 are unknown.…”

Section: Ahr-mediated Activity Of Fractionsmentioning

confidence: 99%

“…However, few data are available to indicate the level of contamination of dioxin-like compounds in these rivers. Sediments are the eventual sink for dioxin-like compounds in the aquatic environment (Berggrena et al, 1999;Hurst et al, 2004), so eleven sites (Fig. 1) were selected along the Haihe River and the Dagu River for sediment sampling and testing.…”

Section: Introductionmentioning

confidence: 99%

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AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

Song

Jiang

et al. 2006

Chemosphere

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Total concentrations of compounds that can cause activation of the aryl hydrocarbon receptor (AhR) in extracts of river sediments from various locations in the Haihe and Dagu Rivers, Tianjin, China were determined by use of the in vitro H4IIE-luc cell line. AhR-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a Florisil column into three Fractions. The response of samples was compared to the TCDD standard and expressed as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQs). Significant dioxin-like activity was observed in each sample. The TCDD-EQs in crude extracts of sediments (SCEs), as determined with the bioassay were 2-4 times greater than the sum of the TCDD-EQs of the eluent from fractions separated with a Florisil column. The results also showed that Fractions 2 and 3 contained most of the AhR-mediated activity. The results obtained by using the bioassay were compared with those of previous measurements of the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) and the structurally-similar AhR-active polychlorinated biphenyls (PCBs). It was determined that sediments from the Dagu River contained greater concentrations of TCDD-EQ than did sediments from the Haihe River except at Jingangqiao (location R3), which is associated with industrial activities in the adjacent densely populated area. The concentrations of TCDD-EQs, based on the EC 20 and the relative potency ranges, of SCEs ranged from 330 to 930 and 1200 to 13 900 pg TCDD-EQ g À1 dry wt in Haihe and Dagu Rivers, respectively.

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Section: Ahr-mediated Activity Of Crude Extracts Of Sedimentssupporting

confidence: 89%

Section: Ahr-mediated Activity Of Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

Song

Jiang

et al. 2006

Chemosphere

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“…We also found that concentrations of BPA in sediments of Xinzh and Dagz were 2 to 3 times higher than those in water. Aquatic sediments serve as a kind of enrichment medium for a number of contaminants and thus can act as an integrative measure of exposure of the aquatic ecosystem to these contaminants (Berggrena et al, 1999;Hurst et al, 2004). In our study, the greatest E2 equivalent values were observed in F3 and the results suggest the presence of estrogenic compounds in F3.…”

Section: Estrogenic Activity Of Sediment Samplessupporting

confidence: 49%

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Song

Jiang

et al. 2006

Environment International

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Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g − 1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 N Fraction 2 N Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 N E1 N NP N OP N BPA.

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“…It uses genetically modified cells (hepatoma cells stably transfected with a luciferase reporter gene) which respond to chemicals that activate the cytosolar AhR by induction of luciferase (Denison et al, 2004). By this method, the toxicity of pollutants such as PCDD/Fs and dioxin like-PCBs is produced either as a change in gene expression mediated through the AhR or by interference with other pathways (Hurst, Balaam, Chan-Man, Thain, & Thomas, 2004). Estimation of relative potency and toxic potential can therefore be done by measuring the activation level of AhR gene expression (United States Environmental protection Agency [USEPA], 2008).…”

mentioning

confidence: 99%

PCDD/F and Dioxin-Like PCB Determinations in Mtoni Estuarine Sediments (Tanzania) Using the Chemically Activated Luciferase Gene Expression (CALUX) Bioassay

Mihale

Croes

Tungaraza

et al. 2013

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Sediments from Mtoni estuary and 2 tributaries, Tanzania, were screened for polychlorinated-p-dibenzodioxins, -dibenzofurans (PCDD/Fs) and dioxin-like PCBs (dl-PCBs) using the chemically activated luciferase gene expression (CALUX) bioassay approach. PCDD/Fs expressed as bio analytical equivalence (BEQ) values ranged from 5.7 ± 1.4 to 39.9 ± 5.8 pg BEQ/g sediment in the wet season and from 14.1 ± 2.0 to 32.8 ± 4.7 pg BEQ/g sediment in the dry season, with higher levels observed in Kizinga River and stations close to the mouth of that river. Dioxin-like PCB levels ranged from 0.21 ± 0.03 to 0.53 ± 0.03 pg BEQ/g sediment in the wet season and from 0.22 ± 0.03 to 0.59 ± 0.04 pg BEQ/g sediment in the dry season. Higher PCDD/F and dl-PCB levels in sediments are probably related to open burning of plastic scraps, household burning of wood or charcoal and traffic related emissions, all of which occur in the Dar es Salaam region. The denser population and the more intense industrial activities in the Kizinga River basin may explain the enhanced PCDD/F and dl-PCB levels observed in the sediments of that river compared to the levels in the Mzinga River basin. A third sampling campaign, including also stations in the downstream estuary, confirmed the enhanced levels in the Kizinga River (maximum of 400 pg-BEQ/g) and also showed that a clear decreasing concentration gradient in the downstream direction exists. It cannot be excluded that the levels of these pollutants in the sediments of the Mtoni estuary pose a threat to the local biological community.

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Determination of dioxin and dioxin-like compounds in sediments from UK estuaries using a bio-analytical approach: chemical-activated luciferase expression (CALUX) assay

Cited by 45 publications

References 35 publications

AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

PCDD/F and Dioxin-Like PCB Determinations in Mtoni Estuarine Sediments (Tanzania) Using the Chemically Activated Luciferase Gene Expression (CALUX) Bioassay

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