Determination of Creatine, Creatinine, and Related Compounds in Urine by Means of Paper Chromatography.

Abstract

As previously stated, the administration of testosterone to many animals including man and the Rhesus monkey, results in an increased urinary excretion of androgens and 17-ketosteroids. In contradistinction to this we noted no increase in either of these substances in the urine of treated Cebus monkeys. This finding parallels those of Dingemanse and Tyslowitz,lo Paschkis et aZ.,ll who found no increase in 10 Dingemanse, E., and Tyslowitz, R., Endocrinology, 1941, 28, 450.--_ _ the urine of dogs similarly treated. In dogsll and man12 increased urinary estrogenic activity has been shown after testosterone treatment. Our results would suggest that in the Cebus monkey as in man and the dog, testosterone can be converted to estrogens, but further work is necessary to elucidate this point.

Creatine and carbohydrate metabolism

“…Chromatographic examination of the liver extracts, however, showed no qualitative difference in amino acid contents of the livers from fed and starved guinea pigs, and no obvious increase in possible amino acid precursors of creatine (glycine, arginine, glutamic acid). Development of the papers by the Jaff6 reaction to demonstrate creatine-like compounds (Ames & Risley, 1948) confirmed the increase of creatine in starvation but failed to show any increase in glycocyammne.…”

Paper chromatography of amines

“…Although the method of paper chromatography has been employed successfully for the separation of amino-acids, sugars, purines, pyrimidines, phenolic compounds and othernaturallyoccurringsubstances, relatively few attempts have so far been made to apply it to the separation and identification of amines. The behaviour of various primary aromatic amines on paper chromatograms has received some attention (Ekman, 1948;Tabone, Robert & Troestler, 1948;Kelemen, Tanos & Halmagyi, 1950), but of the non-aromatic amines only the following groups of biologically important amines have been successfully separated: creatine, creatinine and their demethylated analogues (Maw, 1948;Ames & Risley, 1948); histamine and acetylhistamine (Urbach, 1949); adrenaline, noradrenaline and methyladrenaline (James, 1948); histamine, glucosamine, chondrosamine, choline, and ethanolamine (Dent, 1948;Partridge, 1948;Chargaff, Levine & Green, 1948;Aminoff, Morgan & Watkins, 1950).…”