Paper chromatography of amines

The Chemistry of Ninhydrin.

“…It has the advantage that i t suggests a common route for the reaction of ninhydrin with amines, imino acids, and amino acids, and it explains why these reactions proceed more readily with compounds which contain a carboxyl group adjacent to the nitrogen atom. The orange, brown, gray, and green colors (5,11,24) observed by various workers are almost certainly due to the presence of Schiff bases of the type of XLIX, formed via routes (a) or (d). Indeed, Ruhemann isolated several such condensation products including the compound XLIX (R = phenyl), which is orange in color.…”

Chromatographic methods for the study of amines from biological material

“…Some of the methods have been adapted from those developed for amino acids; others have been worked out specially for amines (on paper: Bremner & Kenten, 1951;Schwyzer, 1952;Herbst, Keister & Weaver, 1958;on ion-exchange resins: Weber, 1951;Gardell, 1953;Kiirshner & Goodall, 1957). Not all of the standard amino acid techniques can be applied to amines: thus electrolytic desalting only works with neutral compounds or ampholytes, and sulphonated polystyrene resins retain amines too firmly for convenient separation, so that rather powerful eluents are needed (Wall, 1953).…”

Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine

“…Purity of the amines was tested by gas chromatography at 1200 on a column containing 70% Carbowax on Chromosorb W (Johns-Manville Co., New York, N.Y., U.S.A.) by using an Aerograph A-600C gas chromatograph (Wilkens Instruments, Walnut Creek, Calif., U.S.A.), or by paper chromatography in butan-1-olacetic acid-water (40:10:50, by vol. ; Bremner & Kenten, 1951). Amines were detected by a ninhydrin spray.…”