Determination of chondroitin sulfate in synovial fluid and drug by ratiometric fluorescence strategy based on carbon dots quenched FAM-labeled ssDNA

Xiao, Jiecheng; Hao, Xiaoli; Miao, Chenfang; Li, Fenglan; Huang, Jianyong; Lin, Xinhua; Chen, Min; Wu, Xianwei; Weng, Shaohuang

doi:10.1016/j.colsurfb.2020.111030

Cited by 11 publications

(3 citation statements)

References 45 publications

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“…45 The shoulder peak was assigned to the specific stretching vibration of C�O at about 1648 cm −1 . 46 The broad bands at 3081 and 3282 cm −1 suggested the existence of amino (−NH 2 ) and hydroxy (−OH) groups. FTIR confirmed the doping effect of the nitrogen element and the presence of the abundance of hydrophilic groups in SP-CDs.…”

Section: T H Imentioning

confidence: 99%

“…In the FTIR spectra (Figure D), the two peaks at 1118 and 1560 cm –1 were attributed to the stretching vibration of C–N–C and the bending vibration of N–H, respectively . The shoulder peak was assigned to the specific stretching vibration of CO at about 1648 cm –1 . The broad bands at 3081 and 3282 cm –1 suggested the existence of amino (−NH 2 ) and hydroxy (−OH) groups.…”

mentioning

confidence: 97%

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Biophilic Positive Carbon Dot Exerts Antifungal Activity and Augments Corneal Permeation for Fungal Keratitis

Chen,

Geng,

Ning

et al. 2024

Nano Lett.

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Fungal keratitis (FK) is an infectious eye disease that poses a significant risk of blindness. However, the effectiveness of conventional antifungal drugs is limited due to the intrinsic ocular barrier that impedes drug absorption. There is an urgent need to develop new therapeutic strategies to effectively combat FK. Herein, we synthesized an ultrasmall positively charged carbon dot using a simple stage-melting method. The carbon dot can penetrate the corneal barrier by opening the tight junctions, allowing them to reach the lesion site and effectively kill the fungi. The results both in vitro and in vivo demonstrated that it exhibited good biocompatibility and antifungal activity, significantly improving the therapeutic effect in a mouse model of FK. Therefore, this biophilic ultrasmall size and positive carbon dot, characterized by its ability to penetrate the corneal barrier and its antifungal properties, may offer valuable insights into the design of effective ocular nanomedicines.

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Section: T H Imentioning

confidence: 99%

mentioning

confidence: 97%

Biophilic Positive Carbon Dot Exerts Antifungal Activity and Augments Corneal Permeation for Fungal Keratitis

Chen,

Geng,

Ning

et al. 2024

Nano Lett.

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“…One of the promising solutions to tackle the low accuracy and stability of the fluorescence detection methods is to use ratiometric fluorescence sensors where the intensities of two or more wavelengths are being measured. Ratiometric fluorescence sensors have higher response stability and have been used widely for the detection of various drugs. − Most of the carbon quantum-dot-based fluorescence sensors rely on single-wavelength fluorescence emission, which has certain limitations . The dual-emissive CQDs are mostly prepared by doping specific elements using complex thermal and hydrothermal routes. , Therefore, there is a growing interest in the preparation of dual-emissive CQDs for ratiometric fluorescence sensors.…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Dual-Emitting Carbon Quantum-Dot-Based Selective Ratiometric Fluorescent Mercaptopurine Detection

Saboorizadeh

Zare‐Dorabei

2022

ACS Biomater. Sci. Eng.

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, an immunosuppressive drug, has been widely prescribed for treating leukemia and autoimmune diseases. The level of the 6-MP drug in body fluids is of great interest due to the severe health problems related to its overdose. This study used a facile microwave preparation route to synthesize carbon quantum dots (CQDs) using glutathione and formamide as carbon sources. The obtained monodispersed quantum dots showed dual fluorescence emission with a sensitive affinity toward the 6-MP drug. The sensor's response was optimized by tuning the temperature, pH, and volume ratio of the probe. The prepared ratiometric fluorescence method showed accurate measurements for determining mercaptopurine in aqueous solutions in the concentration range of 1.4−7.6 mg L −1 with the limit of detection of 1.3 mg L −1 . The sensor's performance was assessed in complex solutions, human urine, and human plasma sample and recovery values in the range of 88− 127% were obtained. The reliable dual fluorometric sensor showed promising results for 6-MP determination and potential application for the determination of other chemical and biochemical species.

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Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155

Zheng

Chen

et al. 2021

Anal Bioanal Chem

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Determination of chondroitin sulfate in synovial fluid and drug by ratiometric fluorescence strategy based on carbon dots quenched FAM-labeled ssDNA

Cited by 11 publications

References 45 publications

Biophilic Positive Carbon Dot Exerts Antifungal Activity and Augments Corneal Permeation for Fungal Keratitis

Biophilic Positive Carbon Dot Exerts Antifungal Activity and Augments Corneal Permeation for Fungal Keratitis

Intrinsic Dual-Emitting Carbon Quantum-Dot-Based Selective Ratiometric Fluorescent Mercaptopurine Detection

Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155

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