Determination of carbohydrates in fermentation processes by high-performance liquid chromatography

Plaga, Armin; Stümpfel, Joachim; Fiedler, Hans‐Peter

doi:10.1007/bf00164821

Cited by 29 publications

(6 citation statements)

References 5 publications

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“…The mobile phase was H 2 SO 4 (5 mM). 42 The flow rate was 0.6 mL/min. Initially, a standard calibration curve for glucose and ethanol were plotted (2.5-50 mM for glucose with the equation of y ¼ 303971.24x; R 2 ¼ 0.999 and 2.5-50 mM for ethanol with the equation of y ¼ 7097.29x; R 2 ¼ 0.994).…”

Section: Apparatusmentioning

confidence: 99%

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel

Akin

Geyik

et al. 2011

Biotechnology Progress

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This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R(2) = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R(2) = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements.

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Section: Apparatusmentioning

confidence: 99%

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel

Akin

Geyik

et al. 2011

Biotechnology Progress

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“…To estimate the concentration of glucose in the fermentation broth, the HPLC method described by Plaga et al 19 was used.…”

Section: Hplc Determination Of Glucosementioning

confidence: 99%

Production of the siderophore enterobactin: Use of four different fermentation systems and identification of the compound by HPLC

Seiffert

Goeke

Fielder

et al. 1993

Biotech & Bioengineering

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The article describes four different fermentation procedures for Escherichia coli AN311, a producer of enterobactin. A regular rotary shaker culture with a biphasic system consisting of an agar layer (as a reservoir for feeding processes) and a layer of liquid medium, 2.4 L and 10 L batch cultures, and a novel dialysis membrane fermentor were used. With the use of this latter fermentor type, the production of enterobactin could be increased by a factor of about 9.5, while growth increased by a factor of 12 compared to the other systems. For the rapid and reliable quantification of the concentration and purity of enterobactin an analytical and preparative high-performance liquid chromatography (HPLC) method was established. The degradation compounds of this siderophore were detected by diodearray and bioassays. A comparison of total catechol production as well as the distribution between enterobactin and its degradation compounds is given.

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“…The sucrose content of the medium was measured by HPLC using an amino-bonded phase as described by Plaga et al (1989).…”

mentioning

confidence: 99%

Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation

Meiwes

Fiedler

Zähner

et al. 1990

Appl Microbiol Biotechnol

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Streptomyces olivaceus TU 2718 produces the siderophore desferrioxamine E. Production depends on L-lysine and iron concentrations in the medium. With optimized conditions the yield of desferrioxamine E could be increased to 12 g/l in feeding fermentations. Supplementation of the basic production medium with natural and synthetic precursors of desferrioxamine E led to the production of twelve new analogues of desferrioxamine E.

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Determination of carbohydrates in fermentation processes by high-performance liquid chromatography

Cited by 29 publications

References 5 publications

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Production of the siderophore enterobactin: Use of four different fermentation systems and identification of the compound by HPLC

Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation

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