Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation

Meiwes, Johannes; Fiedler, Hans‐Peter; Zähner, Hans; Konetschny-Rapp, Silvia; Jung, Günther

doi:10.1007/bf00173718

Cited by 68 publications

(43 citation statements)

References 14 publications

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“…This could be explained by the higher level of ornibactin production in the ornithine-unsupplemented succinate medium observed for these strains ( Table 2). The possibility of increasing siderophore yield by feeding iron-starved growing bacteria with a potential siderophore precursor, as shown in the present study, has already been described for the production of desferriferrioxamine E by Streptomyces olieaceus [Meiwes et al 1990). …”

Section: Discussionmentioning

confidence: 83%

Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia)

et al. 1995

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Several strains of Burkholderia vietnamiensis, isolated from the rhizosphere of rice plants, and four strains formerly known as Pseudomonas cepacia including two collection strains and two clinical isolates were compared for siderophore production and iron uptake. The B. vietnamiensis (TVV strains) as well as the B. cepacia strains (ATCC 25416 and ATCC 17759) and the clinical isolates K132 and LMG 6999 were all found to produce ornibactins under iron starvation. The two ATCC strains of B. cepacia additionally produced the previously described siderophores, pyochelin and cepabactin. Analysis of the ratio of isolated ornibactins (C4, C6 and C8) by HPLC revealed nearly identical profiles. Supplementation of the production medium with ornithine (20 mM) resulted in a 2.5-fold increase in ornibactin synthesis. Ornibactin-mediated iron uptake was independent of the length of the acyl side chain and was observed with all strains of B. vietnamiensis and B. cepacia, but was absent with strains of Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas stutzeri, known to produce pyoverdines or desferriferrioxamines as siderophores. These results suggest that ornibactin production is a common feature of all Burkholderia strains and that these strains develop an ornibactin-specific iron transport system which is distinct from the pyoverdine-specific transport in Pseudomonas strains.

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Section: Discussionmentioning

confidence: 83%

Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia)

et al. 1995

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“…The production of desferrioxamines seems to be conserved throughout streptomycetes (57,58). The desABCD genes that direct the synthesis of these chelating agents (38,59) have been found in all Streptomyces sequenced genomes and in other, related genera, such as Salinispora (60).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas fluorescens Pirates both Ferrioxamine and Ferricoelichelin Siderophores from Streptomyces ambofaciens

Galet

Deveau

Hôtel

et al. 2015

Appl Environ Microbiol

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Iron is essential in many biological processes. However, its bioavailability is reduced in aerobic environments, such as soil. To overcome this limitation, microorganisms have developed different strategies, such as iron chelation by siderophores. Some bacteria have even gained the ability to detect and utilize xenosiderophores, i.e., siderophores produced by other organisms. We illustrate an example of such an interaction between two soil bacteria, Pseudomonas fluorescens strain BBc6R8 and Streptomyces ambofaciens ATCC 23877, which produce the siderophores pyoverdine and enantiopyochelin and the siderophores desferrioxamines B and E and coelichelin, respectively. During pairwise cultures on iron-limiting agar medium, no induction of siderophore synthesis by P. fluorescens BBc6R8 was observed in the presence of S. ambofaciens ATCC 23877. Cocultures with a Streptomyces mutant strain that produced either coelichelin or desferrioxamines, as well as culture in a medium supplemented with desferrioxamine B, resulted in the absence of pyoverdine production; however, culture with a double mutant deficient in desferrioxamines and coelichelin production did not. This strongly suggests that P. fluorescens BBbc6R8 utilizes the ferrioxamines and ferricoelichelin produced by S. ambofaciens as xenosiderophores and therefore no longer activates the production of its own siderophores. A screening of a library of P. fluorescens BBc6R8 mutants highlighted the involvement of the TonB-dependent receptor FoxA in this process: the expression of foxA and genes involved in the regulation of its biosynthesis was induced in the presence of S. ambofaciens. In a competitive environment, such as soil, siderophore piracy could well be one of the driving forces that determine the outcome of microbial competition. Bacteria detect, assimilate, and integrate different environmental signals in order to better adapt to their habitat and cope with changes in environmental conditions. Multiple signaling pathways allow them to communicate with each other within the same species or between different species (1). This can be achieved through the production and detection of diffusible molecules in the environment. In response to these interactions, the microorganisms have developed complex metabolic and physiological responses. One of the essential environmental factors vital for organisms is iron. It plays an essential role in many biological processes, such as DNA synthesis, respiration, and photosynthesis. Iron can adopt two different ionic forms, Fe 2ϩ and Fe 3ϩ . This property makes it an important player in the oxidation-reduction reactions in the cell. However, while iron is an abundant element on earth, its bioavailability is reduced in aerobic environments, such as soil. Ferric iron (Fe 3ϩ ) forms insoluble ferric hydroxides (with a solubility product of ϳ10 Ϫ39 ) in the presence of oxygen (2-4). Therefore, iron is a limiting factor for the growth of microorganisms.To overcome the limitation of iron bioavailability, aerobic bacteria have develop...

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“…The terminal acid residue can also be missing (referred to as "truncated") (115,398). By feeding of suitable diamino precursors to the culture medium unnatural analogs can be obtained (111,194,227). At pH values above 6.5 (Fe 3+ ) 2 Lig 3 complexes prevail, in more acidic media Fe 3+ Lig is formed (194).…”

Section: Hydroxamic Acid Siderophoresmentioning

confidence: 98%

Microbial Siderophores

Budzikiewicz

2010

Fortschritte Der Chemie Organischer Naturstoffe / Progress in the Chemistry of Organic Natural Products, Vol. 92

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Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation

Cited by 68 publications

References 14 publications

Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia)

Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia)

Pseudomonas fluorescens Pirates both Ferrioxamine and Ferricoelichelin Siderophores from Streptomyces ambofaciens

Microbial Siderophores

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