Determination of carbohydrate in lake sediment by a modified phenol-sulfuric acid method

Liu, D.; Wong, Pak Chung; Dutka, B. J.

doi:10.1016/0043-1354(73)90090-0

Cited by 160 publications

(67 citation statements)

References 6 publications

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“…Total carbohydrates were estimated using the phenol-sulphuric acid method (Liu et al 1973) which measures both the sugars and polysaccharides present. Sediment (50 to 100 mg dry wt) was dispersed in 1 ml of deionised water with 10% phenol and sulphuric acid.…”

Section: Methodsmentioning

confidence: 99%

Terrestrial deposits on intertidal sandflats: sediment characteristics as indicators of habitat suitability for recolonising macrofauna

Cummings¹,

Thrush²,

Hewitt³

et al. 2003

Mar. Ecol. Prog. Ser.

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Section: Methodsmentioning

confidence: 99%

Terrestrial deposits on intertidal sandflats: sediment characteristics as indicators of habitat suitability for recolonising macrofauna

Cummings¹,

Thrush²,

Hewitt³

et al. 2003

Mar. Ecol. Prog. Ser.

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“…The pellet was kept at -20°C until analysis. Intracellular carbohydrates were determined using the modified phenol-sulfuric acid method (Liu et al 1973) with glucose as standard. Total intracellular protein was determined according to the procedure of Bradford (1976) using bovine serum albumin (BSA) as standard; protein extraction followed the protocol of Rausch (1981).…”

Section: Methodsmentioning

confidence: 99%

Growth and biochemical composition of Chlorella vulgaris in different growth media

Chia

Lombardi

Melão

2013

An. Acad. Bras. Ciênc.

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The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

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“…1). The colorimetric assay for measuring total carbohydrate concentrations (phenol-sulfuric acid) has a higher response to glucose than to the other monosaccharides in the mucus (Liu et al 1973). …”

Section: Differences Between Mucus Collected At Different Locations (mentioning

confidence: 99%

“…Total carbohydrate concentrations were routinely determined as glucose equivalents by the modified phenol-sulfuric acid method described by Liu et al (1973) using glucose as a standard. In this colorimetric method, the response of different types of monosaccharides varies.…”

Section: Media-medium Composition Was Adapted Frommentioning

confidence: 99%

Microbial breakdown of Phaeocystis mucopolysaccharides

Janse

Rijssel

Ottema

et al. 1999

Limnology & Oceanography

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Mucus from the microalga Phaeocystis was partially purified and used as the C source in various stable enrichment cultures wherein the mucus carbohydrates were degraded under oxic as well as anoxic conditions. The breakdown of mucopolysaccharides (initially at a rate of 50% in 11 d at 12ЊC) markedly slowed after about 15 d, leaving a fraction nondegraded. The carbohydrate composition of the residual fraction did not change during breakdown. It was shown that the incomplete degradation of mucopolysaccharides was not due to inherent resistance to breakdown in parts of the mucus carbohydrates but to the release of inhibitors produced during breakdown. The (potentially high) overall degradability of mucopolysaccharides has important consequences for the fate of C fixed by Phaeocystis. The balance between recycling of Phaeocystis biomass in surface waters and its accumulation, sedimentation, and burial in the sediment will greatly depend on factors such as nutrient availability, presence of inhibiting agents, and composition of the microbial community, rather than on structural impediments inhibiting mucopolysaccharide degradation.

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Determination of carbohydrate in lake sediment by a modified phenol-sulfuric acid method

Cited by 160 publications

References 6 publications

Terrestrial deposits on intertidal sandflats: sediment characteristics as indicators of habitat suitability for recolonising macrofauna

Terrestrial deposits on intertidal sandflats: sediment characteristics as indicators of habitat suitability for recolonising macrofauna

Growth and biochemical composition of Chlorella vulgaris in different growth media

Microbial breakdown of Phaeocystis mucopolysaccharides

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