Growth and biochemical composition of Chlorella vulgaris in different growth media

Abstract

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

Improvement of Nutrition Production by Protoplast Fusion Techniques in Chlorella vulgaris

“…The growth of C. vulgaris and fusant during cultivation share the same pattern for eight day. This pattern are in accordance with Chia et al [26]. The observed growth phases consist of the lag phase, the exponential phase and the stationary phase.…”

“…The process was followed by serial washing in suspension containing 5 mM glycine and 10 mM CaCl 2 . Protoplast regeneration were made by growing the recombinant on Walne medium using sea water containing 5 mM glycine and 10 mM CaCl 2 and incubated for 5-7 days [26,27].…”

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

“…The highest carbohydrate production (7.36 μg·mL -1 ) was observed with the cultures cultivated in LC Oligo under the same conditions. The percentage of total lipid yield was stated to be 10.7 g per 100 gr dry algae [22]. The results obtained by those researchers showed similarities with the results obtained in the present study.…”

“…The vulgaris GUMSH strain (0.051 g/100 mL) while the highest lipid amount was observed in C. vulgaris UIK strain (0.019 g/100 mL) and the lowest in C. vulgaris GUH, TOH, AUH strains (0.010 g/100 mL). Besides, the biochemical composition of C. vulgaris was found as protein (39.2 ± 1.1%), carbohydrates (24.5 ± 0.3%), lipids (25.3 ± 0.1%) in BG-11 nutrient media and as protein (37.2 ± 0.8%), carbohydrates (27.7 ± 0.2%), lipids (26.8 ± 0.2%) in Kolkwitz nutrient media [22,40]. The growth, biomass production and biochemical composition of C. vulgaris cultures were examined in three different media (LC Oligo, Chu 10 and WC media) and the highest cell density (2.74 × 10 6 cells/mL) and chlorophyll a (1 μg/mL) were observed in LC Oligo nutrient medium.…”

“…The 50 mL samples were filtered via Whatman GF/C filter papers and dried at 60 C in incubator and they were weighed [22]. Dry weight determination was made on seventh day following the cultivation.…”