Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor

Mauriz, Elba; Calle, A.; Abad, Antonio; Montoya, Ángel; Hildebrandt, Alain; Barceló, D.; Lechuga, Laura M.

doi:10.1016/j.bios.2005.10.013

Cited by 126 publications

(48 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They produce rapid and reliable results with minimal sample preparation and use of analyte (Elliott et al, 1999;Gaudin et al, 2001;Gustavsson et al, 2002;Haasnoot et al, 2002;Mello and Kubota, 2002;Samsonova et al, 2002;Ferguson et al, 2002Ferguson et al, , 2005Gaudin et al, 2005;Traynor et al, 2006;Mauriz et al, 2006;Llamas et al, 2007;Campbell et al, 2007;Connolly et al, 2007, Campas et al, 2007. This technology allows for real time, automated analysis combining the high affinity of biochemical interactions with low limits of detection and is characterised by its simplicity of use.…”

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Stewart

Elliott

Walker³

et al. 2009

Toxicon

View full text Add to dashboard Cite

a b s t r a c tOkadaic acid (OA) and structurally related toxins dinophysistoxin-1 (DTX-1), and DTX-2, are lipophilic marine biotoxins. The current reference method for the analysis of these toxins is the mouse bioassay (MBA). This method is under increasing criticism both from an ethical point of view and because of its limited sensitivity and specificity. Alternative replacement methods must be rapid, robust, cost effective, specific and sensitive. Although published immuno-based detection techniques have good sensitivities, they are restricted in their use because of their inability to: (i) detect all of the OA toxins that contribute to contamination; and (ii) factor in the relative toxicities of each contaminant. Monoclonal antibodies (MAbs) were produced to OA and an automated biosensor screening assay developed and compared with ELISA techniques. The screening assay was designed to increase the probability of identifying a MAb capable of detecting all OA toxins. The result was the generation of a unique MAb which not only cross-reacted with both DTX-1 and DTX-2 but had a cross-reactivity profile in buffer that reflected exactly the intrinsic toxic potency of the OA group of toxins. Preliminary matrix studies reflected these results. This antibody is an excellent candidate for the development of a range of functional immunochemical-based detection assays for this group of toxins.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Stewart

Elliott

Walker³

et al. 2009

Toxicon

View full text Add to dashboard Cite

show abstract

“…[37][38][39][40][41][42][43][44][45][46] This labelfree, automated analysis technique combines the high affinity of biochemical interactions with low limits of detection producing rapid and reliable results. Minimal amounts of analyte are required to produce the chip surfaces, and these surfaces can be reused many times without loss of activity.…”

mentioning

confidence: 99%

Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

et al. 2009

View full text Add to dashboard Cite

A rapid analytical optical biosensor-based immunoassay was developed and validated for the detection of okadaic acid (OA) and its structurally related toxins from shellfish matrix. The assay utilizes a monoclonal antibody which binds to the OA group of toxins in order of their toxicities, resulting in a pseudofunctional assay. Single-laboratory validation of the assay for quantitative detection of OA determined that it has an action limit of 120 μg/kg, a limit of detection of 31 μg/kg, and a working range of 31−174 μg/kg. The midpoint on the standard matrix calibration curve is 80 μg/kg, half the current regulatory limit. Inter- and intra-assay studies of negative mussel samples spiked with various OA concentrations produced average coefficient of variation (CV) and standard deviation (SD) values of 7.9 and 10.1, respectively. The assay was also validated to confirm the ability to accurately codetect and quantify dinophysistoxin-1 (DTX-1), DTX-2, and DTX-3 from shellfish matrix. Alkaline hydrolysis was not required for the detection of DTX-3 from matrix. Excellent correlations with the data generated by the biosensor method and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were obtained using a certified reference material (R 2 = 0.99), laboratory reference material, and naturally contaminated mussel samples (R 2 = 0.97). This new procedure could be used as a rapid screening procedure replacing animal-based tests for DSP toxins.

show abstract

“…Taking advantage of QCM measurements based on the phase/mass approach above described, in the present work we report the development of an HFF-QCM immunosensor for the highsensitivity determination of carbaryl and thiabendazole pesticides. Carbaryl, the first successful carbamate insecticide with a broad-spectrum efficacy, was chosen as a model analyte because it has become a reference analyte for immunoassay comparison, from ELISA to immunosensors March et al, 2009;Mauriz et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

High-frequency phase shift measurement greatly enhances the sensitivity of QCM immunosensors

March

García

Sánchez

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor

Cited by 126 publications

References 17 publications

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

High-frequency phase shift measurement greatly enhances the sensitivity of QCM immunosensors

Contact Info

Product

Resources

About