Determination of bromhexine in cough–cold syrups by absorption spectrophotometry and multivariate calibration using partial least-squares and hybrid linear analyses. Application of a novel method of wavelength selection

Goicoechea, Héctor C.

doi:10.1016/s0039-9140(99)00080-6

Cited by 48 publications

(21 citation statements)

References 21 publications

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“…Additional selected examples involving the use of error-propagation for estimating concentration uncertainties and limit of detection are the spectrofluorometric determination of the antibiotic tetracycline in human serum [98,145] and the spectrophotometric analysis of bromhexine in a decongestant syrup [98,165]. In the former case, the analyte signal is completely overlapped by the stronger and intrinsically variable fluorescence bands of human serum, see Fig.…”

Section: Formula-basedmentioning

confidence: 99%

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Uncertainty estimation and figures of merit for multivariate calibration (IUPAC Technical Report)

Olivieri

Faber²,

Ferré

et al. 2006

Pure and Applied Chemistry

315

164

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This paper gives an introduction to multivariate calibration from a chemometrics perspective and reviews the various proposals to generalize the well-established univariate methodology to the multivariate domain. Univariate calibration leads to relatively simple models with a sound statistical underpinning. The associated uncertainty estimation and figures of merit are thoroughly covered in several official documents. However, univariate model predictions for unknown samples are only reliable if the signal is sufficiently selective for the analyte of interest. By contrast, multivariate calibration methods may produce valid predictions also from highly unselective data. A case in point is quantification from near-infrared (NIR) spectra. With the ever-increasing sophistication of analytical instruments inevitably comes a suite of multivariate calibration methods, each with its own underlying assumptions and statistical properties. As a result, uncertainty estimation and figures of merit for multivariate calibration methods has become a subject of active research, especially in the field of chemometrics.

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Section: Formula-basedmentioning

confidence: 99%

“…In the analysis of bromhexine in pharmaceutical syrups [165], the strong absorption of the background syrup does also seriously overlap with the analyte signal, see Fig. 12.…”

Section: Formula-basedmentioning

confidence: 99%

Uncertainty estimation and figures of merit for multivariate calibration (IUPAC Technical Report)

Olivieri

Faber²,

Ferré

et al. 2006

Pure and Applied Chemistry

315

164

View full text Add to dashboard Cite

show abstract

“…In the second experimental example the analyte bromhexine is spectrophotometrically determined within the complex mixture of excipients of a decongestant syrup [29]. The optimal OSC model in this case requires the extraction of two factors from the data matrix X (i.e.…”

Section: Experimental Data Setsmentioning

confidence: 99%

“…UV spectra were acquired on a Beckman DU-640 spectrophotometer, using 1.00 cm quartz cells, in the wavelength range 285±348 nm, digitized every 1.0 nm (64 points per spectrum). For further details see Reference [29].…”

mentioning

confidence: 99%

A simple approach to uncertainty propagation in preprocessed multivariate calibration

Olivieri

2002

Journal of Chemometrics

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A simple approach is described to estimate the confidence limit for the concentrations predicted by multivariate calibration when preprocessing techniques such as orthogonal signal correction or net analyte calculation are applied. It involves reconstructing the unpreprocessed data using the extracted spectral factors and those employed for prediction in order to correctly estimate the sample leverages. Monte Carlo simulations carried out by adding random noise to both concentrations and analytical signals for theoretical binary mixtures are in excellent agreement with the calculations. Experimental multicomponent examples were studied by a similar Monte Carlo approach, and the obtained variances are also in agreement with the calculated values. Implications concerning the limits of detection in the latter cases are also discussed.

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“…Bromhexine hydrochloride is am ucolytic used in the treatment of respiratory disorders associated with productive cough.I th as been determined by different techniques includings pectrophotometry [1,2 ,3 ], HPLC [4,5 ,6 ], colorimetry [ 7,8], TLC [ 9],F low-injection-spectrophotometry [ 10], GC [11],I on-Selective Electrode (ISE) [ 12], Hybrid Linear Analysis [13],c apillary isotachophoresis [14],A bsorption Spectrophotometry and Electrophoresis [15,16].…”

Section: Introductionmentioning

confidence: 99%

Potentiometric Flow Injection Analysis of Bromhexine Hydrochloride and its Pharmaceutical Preparation Using Conventional and Coated Wire Ion-Selective Electrodes

2006

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Bromhexine hydrochloridei on-selectivee lectrodes (conventionalt ype)b ased on bromhexinium tetraphenylb orate( I) andb romhexinium-phosphotungstate (II) were prepared.T he electrodes exhibitedm ean slopes of calibration graphs of 59.4 mV and 59.8 mV per decade of bromhexine concentrationa t2 5 Cf or electrode (I) and (II),r espectively.B oth electrodes couldb eu sed within the concentrationr ange 3.16x10-5 -1.00x10 -2 Mb romhexine within the pH range 2.0-4.5. The standard electrodes potentials were determined at different temperatures and used to calculate the isothermal coefficients of the electrodes, which were 0.00065 and 0.00050V C -1 for (I) and (II), respectively. The electrodes showed av ery good selectivity for bromhexine with respect to an umber of inorganic cations, amino acids and sugars. The electrodes were applied to the potentiometric determination of bromhexine hydrochloride and its pharmaceutical preparationu nder batch and flow injection conditions.G raphite, copper and silver coated wires were prepared, characterized and successfully applied as sensors for the drug under investigation. KeywordsIon-selectivee lectrodes, bromhexine hydrochloride, conventional andc oated wire electrodes,flowinjection analysis andconductimetry.Scientia Pharmaceutica (Sci. Pharm.) 74, 121-135

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Determination of bromhexine in cough–cold syrups by absorption spectrophotometry and multivariate calibration using partial least-squares and hybrid linear analyses. Application of a novel method of wavelength selection

Cited by 48 publications

References 21 publications

Uncertainty estimation and figures of merit for multivariate calibration (IUPAC Technical Report)

Uncertainty estimation and figures of merit for multivariate calibration (IUPAC Technical Report)

A simple approach to uncertainty propagation in preprocessed multivariate calibration

Potentiometric Flow Injection Analysis of Bromhexine Hydrochloride and its Pharmaceutical Preparation Using Conventional and Coated Wire Ion-Selective Electrodes

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