Determination of bovine rotavirus G genotypes in Kashmir, India

Wani, S.A.; Bhat, Mashooq A.; Ishaq, S. M.; Ashrafi, Mahboobeh

doi:10.20506/rst.23.3.1538

Cited by 16 publications

(21 citation statements)

References 18 publications

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“…Observations from the present study are in contrast to ones from previous reports [11,14,20,22,27,28,33,34] on the predominance of G10 and G6. During the present study none of the isolate was typed as G6 while a number of isolates were genotyped as G10 type but detected only in combination of G3 type.…”

Section: Discussioncontrasting

confidence: 99%

“…Previous studies from India have shown a predominance of G10 in contrast to other G types (G6 or G8) among diarrhoeic calves [14,20,22,27,28,34]. However, a report from eastern India found a predominance of G6 rather than G10 as G6 was detected in 89.2% cases and G10 only in 4.6% cases [11].…”

Section: Discussionmentioning

confidence: 96%

“…In India, G10 (50~60%) has been the predominant bovine RV genotype over the last 10 years followed by G6 (25~35%) [11,14,20,22,27,28,34]. Varshney et al [33] were the first to report on the existence of G3 RV in buffalo calves (10.2%) in central India.…”

Section: Introductionmentioning

confidence: 99%

“…This was the second most predominant strain after G10. In 2007, Ghosh et al [11] also reported a 3% incidence of G3 RVs in claves from eastern India, indicating that either the number of bovines infected with RV G3 genotypes are increasing or these animals have not been taken into account while conducting genotyping surveys in the country [14,20,22,27,28,34]. The present study describes G and P genotypes prevalent in Indian bovine populations from diverse tropical, sub-tropical/semi-arid, and temperate western Himalayan regions during 2007~2010.…”

Section: Introductionmentioning

confidence: 99%

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Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

et al. 2012

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The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type. Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970, and BR-133 showed that these strains had sequence identities of 90.5% to 100% with other bovine G3 strains. The highest identity (98.9% to 100%) was observed with RUBV3 bovine G3 strains from eastern India. The G3 strains (B-46, 0970, and BR-133) showed 97.5% to 98.8% sequence homologies with the Indian equine RV strain Erv-80. Phylogenetic analysis demonstrated that G3 strains clustered with bovine RUBV3 and J-63, and equine Erv-80 G3. Overall, these results confirmed that the incidence of infection by RVs with the G3 genotype and mixed genotypes in the bovine population was higher than previously predicted. This finding reinforces the importance of constantly monitoring circulating viral strains with the G3 genotype in future surveillance studies.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

et al. 2012

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“…[16] The lack of detection of Campylobacter isolates in cattle in our study could either be due to a low burden among these animals or due to the presence of inhibitors affecting PCR on animal faeces. [17,18] Additional techniques, including culture on enrichment and/or semi-selective media or on template DNA obtained from enrichment medium may have improved detection rates.…”

Section: Discussionmentioning

confidence: 99%

Detection and species identification of Campylobacter in stool samples of children and animals from Vellore, south India

Rajendran

Babji

George

et al. 2012

Indian Journal of Medical Microbiology

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Campylobacter spp. are an important cause of bacterial gastroenteritis frequently isolated from animal, poultry and environmental samples. In this study, we investigated the zoonotic potential of Campylobacter spp. by comparing prevalence rates and species in 394 children with diarrhoea and 652 animals in Vellore using PCR-based tools. Eighteen children (4.5%) had campylobacteriosis, a majority of whom had co-pathogens (15/18) and most were infected with Campylobacter jejuni (16/18). A few C. coli and mixed infections with both species were also seen. Among the animal samples, 16/25 chicken samples (64%) were positive and all were found to be C. jejuni.

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Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Raorane

Dubal

Ghatak

et al. 2020

Heliyon

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Introduction Rotavirus is the leading cause of diarrhoea in young children in India, responsible for an estimated 21357 mean numbers of deaths in 2010. Various genotypes of rotaviruses evolved due to mutational changes have been recognized. In this study, we determined the genotypes of rotaviruses involved in diarrhea in Goa and Meghalaya states of India. Methods The dsRNA of rotaviruses was extracted from stool samples and detected by Ribonucleic Acid-Polyacrylamide gel electrophoresis (RNA PAGE) and Reverse transcription-polymerase Chain Reaction (RT-PCR) targeting the partial VP7 gene. The full length VP7 and partial VP4 genes of rotavirus strains were amplified by RT-PCR followed by nucleotide sequencing. The RotaC classification tool was used to determine the genotypes. Results The positivity of rotavirus by PAGE and RT-PCR was observed to be 43.10% and 39.65% in Goa and 38% and 36% in Meghalaya, respectively. Though long electrophoretic profile was appeared to be the most predominant rotavirus type in circulation in these two states, 96% of long and 84.61% short electropherotype profiles could be detected by RT-PCR. The dsRNA of rotavirus extracted from 36 samples could be transcribed and amplified by beg9end9 primers for G genotyping, while, 41 by con3con2 primers for P genotyping. G1P[8] and G1P[6] genotypes were commonly circulated in Goa and G1P[8] and G1P[4] genotypes in Meghalaya. On nucleotide analysis, 6 samples from Goa showed G1 genotype specificity, while, 3 showed P[8] specificity indicating the G1P[8] rotavirus circulating in Goa. In Meghalaya state, 3 strains showed P[8] and 2 showed P[4] genotype specificity. The majority of the G and P genotypes were closely related to each other and G1 genotypes appeared in two separate clusters, while, P[8] and P[4] appeared in the respective clusters. Conclusion The circulation of G1P[8], G1P[6] genotypes in Goa and the presence of G1P[8] and G1P[4] genotypes in Meghalaya was observed.

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Determination of bovine rotavirus G genotypes in Kashmir, India

Cited by 16 publications

References 18 publications

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Detection and species identification of Campylobacter in stool samples of children and animals from Vellore, south India

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

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