Detection and species identification of Campylobacter in stool samples of children and animals from Vellore, south India

Rajendran, Priya; Babji, Sudhir; George, AT; Rajan, DP; Kang, Gagandeep; Ajjampur, S. S. R.

doi:10.4103/0255-0857.93049

Cited by 29 publications

(10 citation statements)

References 15 publications

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“…Mixed infections involving Campylobacter have been described previously; one large (n = 3,038) surveillance study of diarrhoea in hospitalised children <5 years of age in Bangladesh reported that 59% of all Campylobacter infections were associated with at least one other bacterial or protozoan pathogen [25]. Few studies have reported mixed infections of Campylobacter and viral pathogens; a cohort study in India demonstrated that rotavirus was associated with 11/18 Campylobacter infections in children <5 years of age [26]. Since data from in-vitro cell culture systems suggest that viral co-infection increases the adhesion and invasion of pathogenic Campylobacter species [27], further work should explore the clinical consequences of co-infection with viral enteric pathogens on the pathogenesis of Campylobacter infection.…”

Section: Discussionmentioning

confidence: 93%

Campylobacter Infection in Children in Malawi Is Common and Is Frequently Associated with Enteric Virus Co-Infections

et al. 2013

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Background Campylobacter species are the most common cause of bacterial gastroenteritis in the developed world. However, comparatively few studies have determined the epidemiological features of campylobacteriosis in resource-poor settings.MethodsA total of 1,941 faecal specimens collected from symptomatic (diarrhoeic) children and 507 specimens from asymptomatic (non-diarrhoeic) children hospitalised in Blantyre, Malawi, between 1997 and 2007, and previously tested for the presence of rotavirus and norovirus, was analysed for C. jejuni and C. coli using a real time PCR assay.Results Campylobacter species were detected in 415/1,941 (21%) of diarrhoeic children, with C. jejuni accounting for 85% of all cases. The median age of children with Campylobacter infection was 11 months (range 0.1–55 months), and was significantly higher than that for children with rotavirus and norovirus (6 months and 7 months respectively; P<0.001). Co-infection with either rotavirus or norovirus was noted in 41% of all cases in the diarrhoeic group. In contrast, the detection rate of Campylobacter in the non-diarrhoeic group was 14%, with viral co-infection identified in 16% of children with Campylobacter. There was no association between Campylobacter detection rate and season over the 10 year period.DiscussionUsing molecular detection methodology in hospitalised Malawian children, we have demonstrated a high prevalence of Campylobacter infection, with frequent viral co-infection. The burden of Campylobacter infection in young African children may be greater than previously recognised.

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Section: Discussionmentioning

confidence: 93%

Campylobacter Infection in Children in Malawi Is Common and Is Frequently Associated with Enteric Virus Co-Infections

et al. 2013

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“…In contrast to these findings, many workers including Rajendran et al . [22] and Deckert et al . [23] have reported the higher presence of C. jejuni than C. coli .…”

Section: Discussionmentioning

confidence: 99%

Virulence typing and antibiotic susceptibility profiling of thermophilic Campylobacters isolated from poultry, animal, and human species

et al. 2018

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Background and Aim:Campylobacteriosis finds its place among the four important global foodborne illnesses. The disease, though self-limiting, needs antibacterial therapy in extraintestinal complications. Therefore, the present study was designed to estimate the prevalence of thermophilic Campylobacters in poultry, animals, and humans of the Kumaon region of Uttarakhand.Materials and Methods:A total of 609 samples comprising of poultry ceca (n=116), poultry droppings (n=203), and feces of pigs (n=71), cattle (n=61), sheep (n=19), goat (n=17), human beings (n=88), and laboratory animals (n=34) (rats, rabbits, and guinea pigs) were collected. The thermophilic Campylobacters, Campylobacter jejuni and Campylobacter coli were confirmed using multiplex polymerase chain reaction. The isolates were also screened for the presence of virulence genes, and their antibiotic susceptibility testing was done against eight antibiotics.Results:An overall prevalence of 6.24% was revealed with highest from poultry ceca (15.52%), followed by poultry droppings (5.91%), cattle feces (4.92%), human stools (3.40%), and pig feces (2.82%). The virulence genes, namely cadF, flaA, virB11, and pldA, were present in 38 (100%), 37 (97.37%), 7 (18.42%), and 14 (36.84%) isolates, respectively. All the isolates were resistant to nalidixic acid, while all were sensitive to erythromycin and co-trimoxazole.Conclusion:It was concluded that the animals and humans in the region harbored the thermophilic Campylobacters which may contribute to the human illness. Resistance shown among the isolates may complicate the antimicrobial therapy.

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“…Campylobacteriosis has also been reported to be most prevalent in children under the age of 5 years in this region (isolated in 10% of cases, compared to 3.7% for other age groups; P Ͻ 0.001) (63). In Vellore, South India, between January 2003 and May 2006, 4.5% of 349 children under the age of 5 years with diarrhea were positive by PCR for C. jejuni or C. coli (65). In addition, in a prospective case-control study conducted between 1 December 2007 and 3 March 2011 to identify the etiology of diarrhea in children aged 0 to 59 months, C. jejuni was reported to be significantly associated with moderate to severe diarrhea in children from Kolkata, India, Mirzapur, Bangladesh, and Karachi, Pakistan (66).…”

Section: Epidemiologymentioning

confidence: 96%

Global Epidemiology of Campylobacter Infection

et al. 2015

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SUMMARY Campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. The incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. The dramatic increase in North America, Europe, and Australia is alarming, and data from parts of Africa, Asia, and the Middle East indicate that campylobacteriosis is endemic in these areas, especially in children. In addition to C. jejuni , there is increasing recognition of the clinical importance of emerging Campylobacter species, including Campylobacter concisus and Campylobacter ureolyticus . Poultry is a major reservoir and source of transmission of campylobacteriosis to humans. Other risk factors include consumption of animal products and water, contact with animals, and international travel. Strategic implementation of multifaceted biocontrol measures to reduce the transmission of this group of pathogens is paramount for public health. Overall, campylobacteriosis is still one of the most important infectious diseases that is likely to challenge global health in the years to come. This review provides a comprehensive overview of the global epidemiology, transmission, and clinical relevance of Campylobacter infection.

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Detection and species identification of Campylobacter in stool samples of children and animals from Vellore, south India

Cited by 29 publications

References 15 publications

Campylobacter Infection in Children in Malawi Is Common and Is Frequently Associated with Enteric Virus Co-Infections

Campylobacter Infection in Children in Malawi Is Common and Is Frequently Associated with Enteric Virus Co-Infections

Virulence typing and antibiotic susceptibility profiling of thermophilic Campylobacters isolated from poultry, animal, and human species

Global Epidemiology of Campylobacter Infection

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