Determination of benomyl residues in soils and plant tissues by high-speed cation exchange liquid chromatography

Kirkland, J. J.; Holt, R. F.; Pease, Harlan L.

doi:10.1021/jf60187a037

Cited by 59 publications

(24 citation statements)

References 3 publications

(5 reference statements)

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“…Alternatively, extraction may be carried out using a more polar organic solvent without pH manipulation. Some researchers have extracted these drug residues using a single solvent system, such as acetonitrile [119,134], or binary water/organic solvent mixtures which would be around neutral pH [131,[135][136][137]. Extraction with acidified extractants has only been used by a small number of researchers for methods requiring hydrolysis or digestion steps [101,125,136,137] or methods including only a limited number of residues [138].…”

Section: Extraction and Clean-up Of Benzimidazole Residuesmentioning

confidence: 99%

“…Kirkland et al [136] extracted BEN from soil and crops by refluxing in the presence of methanol and 1N HCl. Hydrolysed extracts were purified by a series of LLP steps prior to HPLC determination.…”

Section: Plant Matricesmentioning

confidence: 99%

“…Alvinerie and Galtier [149] developed a normal phase separation of TCB, TCB-SO, TCB-SO 2 and OFZ on a silica column achieving good separation in a 15 min run. Kirkland et al [136] used a cation exchange column for determination of BEN residues in plants and soil. Arenas and Johnson [126] determined TBZ and 5-OH-TBZ in milk using a strong cation exchange column.…”

Section: Liquid Chromatography Based Separationsmentioning

confidence: 99%

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Review of methodology for the determination of benzimidazole residues in biological matrices

Danaher

Ruyck

Crooks

et al. 2007

Journal of Chromatography B

185

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Section: Extraction and Clean-up Of Benzimidazole Residuesmentioning

confidence: 99%

Section: Plant Matricesmentioning

confidence: 99%

Section: Liquid Chromatography Based Separationsmentioning

confidence: 99%

See 1 more Smart Citation

Review of methodology for the determination of benzimidazole residues in biological matrices

Danaher

Ruyck

Crooks

et al. 2007

Journal of Chromatography B

185

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“…The breakdown of benomyl in aqueous solution to carbendazim (methyl 1 H-benzimidazol-2-ylcarbamate or MBC), during the analytical procedure is well documented (Kirkland et al, 1973). Benomyl is also decomposed to carbendazim in organic solvents as reported by Chiba and Doombos (1974), who found that only 0.6% of the benomyl remained intact after 5.6 hours storage in methanol.…”

Section: Sensitivity and Specificitymentioning

confidence: 87%

Sensitivity, specificity and predictive value for the analysis of benomyl, as carbendazim, on field-treated strawberries using different enzyme linked immunosorbent assay test kits

Lissemore

Ripley

Kakùda

et al. 1996

J. of Env. Sc. & Hlth., Part B

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The primary objective of this study was to evaluate the feasibility of enzyme linked immunosorbent assay (ELISA) for the determination of benomyl in fieldtreated samples, as part of a multi-residue screen for pesticides. The rate of false positive and negative results using ELISA as part of a monitoring program is a primary concern in determining its suitability as a screening tool. Sensitivity and specificity of ELISA for benomyl residues in field-treated strawberries was determined using two different immunosorbent assays and the results compared to 871 872 LISSEMORE ET AL.HPLC. The relative precision and agreement in quantitation for the three analyses was also examined.The Ohmicron Benomyl/Carbendazim Rapid Assay established sensitivity and specificity values of 100%, as did the HPLC methods. The Millipore Benomyl/Carbendazim test kit had sensitivity and specificity values of 93.3% and 51.9%, respectively. This was optimized to 86.6% and 94.2% by raising the minimum reporting detection limit to 0.050 µg/g. While precision for the ELISA test kits, using strawberries, was not as good as the HPLC, the less extensive cleanup involved with ELISA analysis resulted in comparable overall variations. Agreement of quantitation for the three tests was significantly different.The sensitivity and specificity results obtained from the Ohmicron Rapid Assay, after clean up with the Ohmicron Food Prep Kit, supported its use as a potential screening tool for benomyl as carbendazim. It is less labour intensive than HPLC methods and provides a relatively quick screen for benzimidazole compounds in general. Given uncertainty of quantitation obtained from some matrices however, confirmation of positive results using HPLC is still highly recommended.

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“…Several methods have been reported on the residue analysis of TM and its metabolite, methyl benzimidazol-2-ylcarbamate (MBC), in plants and soils using UV-spectrophotometry, 1-5) fluorescence spectrophotometry, 6, 7) colorimetry, 8) thin layer chromatography, [9][10][11][12][13][14] gas chromatography15-17> and high performance liquid chromatography (HPLC), [18][19][20][21][22][23][24] Recently these methods have been reviewed. 25) In the course of the residue analysis of TM, some loss of TM in homogenized samples in storage and low recoveries from samples such as unpolished rice, cooked polished rice and soils were experienced when various samples were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Preventive Effect of Sodium L-Ascorbate for Decomposition of Thiophanate-methyl in Homogenized Crops

Shiga

Kinoshita

et al. 1984

J. Pestic. Sci.

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Effect of sodium L-ascorbate (SL-A) on the residue analysis of thiophanate-methyl (TM) is described. TM in homogenized samples of grape, apple, tomato, and so on, was considerably unstable in storage, and the recoveries from samples such as unpolished rice, cooked polished rice and soil were low (24-61%).Addition of SL-A to homogenized samples increased the stability of TM during storage and analytical procedure, and resulted consequently in good recoveries (78-97%) and also improved precision of data. It was suggested that oxidative decomposition was responsible for the instability of TM, because several antioxidants made TM stable in homogenized grape. The decomposition of TM in homogenized grapes was dependent on incubation temperature and pH, and it required heat-labile component in the grape. Either catalase or sodium azide prevented the decomposition of TM in homogenized grapes. These results evidenced that oxidative decomposition of TM occurred enzymatically in the homogenized preparation of the grape and this decomposition was inhibited by SL-A.

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Determination of benomyl residues in soils and plant tissues by high-speed cation exchange liquid chromatography

Cited by 59 publications

References 3 publications

Review of methodology for the determination of benzimidazole residues in biological matrices

Review of methodology for the determination of benzimidazole residues in biological matrices

Sensitivity, specificity and predictive value for the analysis of benomyl, as carbendazim, on field-treated strawberries using different enzyme linked immunosorbent assay test kits

Preventive Effect of Sodium L-Ascorbate for Decomposition of Thiophanate-methyl in Homogenized Crops

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