Review of methodology for the determination of benzimidazole residues in biological matrices

Danaher, Martin; Ruyck, Hendrik De; Crooks, Steven R. H.; Dowling, Geraldine; O’Keeffe, Michael

doi:10.1016/j.jchromb.2006.07.046

Cited by 184 publications

(132 citation statements)

References 194 publications

(297 reference statements)

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“…Cytosolic ketone reductases were included in our study because a reduction of the carbonyl group gives rise to the main FLU metabolite (FLUR) in the first phase of biotransformation [14,18,31]. Moreover, aldo-keto reductases together with short-chain dehydrogenases/reductases regulate a wide range of physiological processes including reproduction, development and homeostasis.…”

Section: Discussionmentioning

confidence: 99%

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Activities of biotransformation enzymes and flubendazole metabolism in lambs (Ovis aries): effect of gender and flubendazole therapy

Bártíková

Křížová

Štěpničková

et al. 2010

Pharmacological Reports

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Abstract:The effect of flubendazole (FLU) therapy on in vitro FLU biotransformation and the activities of selected biotransformation enzymes were investigated in male and female lambs. Four experimental groups were used: control (untreated) ewes and rams and FLU-treated ewes and rams (orally, 15 mg/kg per day, for three consecutive days). Subcellular fractions were prepared from liver and intestinal mucosa 24 h after the final dosage was administered. Activities of cytochromes P450 (CYP), flavine monooxygenases (FMO), carbonyl reducing enzymes, UDP-glucuronosyl transferase (UGT) and glutathione S-transferase were tested. Significant gender differences were observed for FMO-mediated activity (2-fold higher in ram lambs) and UGT activity (up to 30% higher in ewe lambs), but no gender differences were observed in FLU metabolism. FLU-treatment of lambs moderately changed the activities of some CYPs, FMO, and UGT in liver microsomes. In vitro FLU reduction was not altered in the liver, but was slightly higher in the small intestine of FLU pre-treated lambs. This correlated with the higher carbonyl reductase activities measured in the gut mucosa of these animals.

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Section: Discussionmentioning

confidence: 99%

“…Reduction of the carbonyl group (reduced flubendazole, FLUR) and carbamate hydrolysis (hydrolyzed flubendazole, FLUH) were the major biotransformation reactions [14,20,33].…”

Section: Introductionmentioning

confidence: 99%

Activities of biotransformation enzymes and flubendazole metabolism in lambs (Ovis aries): effect of gender and flubendazole therapy

Bártíková

Křížová

Štěpničková

et al. 2010

Pharmacological Reports

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“…Benzimidazoles are veterinary drugs widely used for the prevention and treatment of parasitic infections in agriculture and aquaculture, including food producing animals (Danaher, 2007). The extensive use of benzimidazoles in lactating animals could result in the presence of their residues in milk and dairy products, which is harmful to consumers owing to their teratogenic and embryotoxic properties (Chen et al, 2010.).…”

Section: Introductionmentioning

confidence: 99%

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

et al. 2017

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A new analytical method using high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the analysis of 18 benzimidazoles and their metabolites in milk. Samples were extracted with acetonitrile and n-hexane and purified by polymer cation exchange (PCX) solid phase extraction cartridges. LC separation was performed on Xbridge C18 with gradient elution using acetonitrile and ammonium acetate buffer. The DAD detection was set at 298, 312, 254 and 290 nm. The method was validated according to the criteria of Commission Decision 2002/657/EC. The following validation parameters were set: accuracy (expressed as recovery) 31.7-137.6 %, limit of decision (CCα) 6.0-120.6 µg kg -1 , detection capability (CCβ) 6.1-120.8 µg kg , precision as CV 7.0-22.5 %, withinlaboratory reproducibility expressed as CV 8.8-30.6 %. Finally, the developed method was applied to the analysis of collected milk samples. A total of 50 milk samples was analysed for benzimidazole residues. All obtained concentrations for all compounds were below the LOQ values.

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“…[15][16][17] However, there may be a concern that if withdrawal periods are not adhered to, or if products are administered to animals in unapproved applications, the levels may exceed maximum residue limits (MRLs) in foods. 15 The MRL values range from 10 to 5000 µg kg -1 depending on the compound and biological matrix. 18 Therefore, the development of sensitive, effective, and reliable analytical methods is still required to monitor these residues in food samples.…”

Section: Introductionmentioning

confidence: 99%

Cationic Micellar Precipitation for Simultaneous Preconcentration of Benzimidazole Anthelmintics in Milk Samples by High-Performance Liquid Chromatography

Vichapong¹,

Santaladchaiyakit²,

Srijaranai³

et al. 2016

J. Braz. Chem. Soc.

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A method using micellar precipitation process using cationic surfactant cetyltrimethyl ammonium bromide at ambient temperature was investigated for simultaneous determination of benzimidazoles coupled with high-performance liquid chromatography (HPLC) analysis. The studied benzimidazoles was selected as model compounds including thiabendazole, albendazole, mebendazole, and fenbendazole. The experimental parameters affected the extraction efficiency, including the kind and concentration of salt, pH, concentration of CTAB, volume of 1-octanol, and the centrifugation extraction time, was optimized. The optimum extraction condition: 0.030 mol L , with correlation coefficients more than 0.999. Finally, the proposed method was successfully applied in the analysis of benzimidazoles in milk samples.

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Review of methodology for the determination of benzimidazole residues in biological matrices

Cited by 184 publications

References 194 publications

Activities of biotransformation enzymes and flubendazole metabolism in lambs (Ovis aries): effect of gender and flubendazole therapy

Activities of biotransformation enzymes and flubendazole metabolism in lambs (Ovis aries): effect of gender and flubendazole therapy

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

Cationic Micellar Precipitation for Simultaneous Preconcentration of Benzimidazole Anthelmintics in Milk Samples by High-Performance Liquid Chromatography

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