Cationic Micellar Precipitation for Simultaneous Preconcentration of Benzimidazole Anthelmintics in Milk Samples by High-Performance Liquid Chromatography

Abstract

A method using micellar precipitation process using cationic surfactant cetyltrimethyl ammonium bromide at ambient temperature was investigated for simultaneous determination of benzimidazoles coupled with high-performance liquid chromatography (HPLC) analysis. The studied benzimidazoles was selected as model compounds including thiabendazole, albendazole, mebendazole, and fenbendazole. The experimental parameters affected the extraction efficiency, including the kind and concentration of salt, pH, concentration of CTAB, volume of 1-octanol, and the centrifugation extraction time, was optimized. The optimum extraction condition: 0.030 mol L , with correlation coefficients more than 0.999. Finally, the proposed method was successfully applied in the analysis of benzimidazoles in milk samples.

Review of Characteristics and Analytical Methods for Determination of Thiabendazole

“…Several different animal tissues were used as samples, and results revealed that this improved method could be a great choice for the determination of benzimidazoles in tissue samples. A few years later, the same group of authors [171] improved the extraction using surfactants in the sample preparation. The proposed extraction technique was performed at room temperature with cationic surfactant cetyltrimethyl ammonium bromide in the absence of dispersive solvent.…”