Determination of baicalin, chlorogenic acid and caffeic acid in traditional chinese medicinal preparations by capillary zone electrophoresis

Abstract

SummaryA capillary zone electrophoresis (CZE) method was developed for the simultaneous assay of three bioactive components -baicalin, chlorogenic acid and caffeic acid -in seven traditional Chinese medicinal preparations. The analytes were separated successfully within 3.5 min using 10 mM borate buffer (pH 8.6). Regression equations revealed linear relationships (correlation coefficients 0.9942-0.9996) between the peak area and concentration of the three analytes. The relative standard deviations of the migration times and the peak areas of the three constituents were 1.12-2.68% and 1.62-5.73%, respectively. Recovery of the three constituents ranged from 89 to 107 %. The extraction efficiencies of different extraction solutions are also discussed. The contents of the three components in seven different Chinese medicinal preparations containing Honeysuckle flower and/or Scutellariae radix were determined by the CZE method with satisfactory results.

CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations

“…The relative standard deviation (RSD) was 2.2% at a concentration of 1.0 x 10 −5 g/L ( n = 11). Compared to other methods reported as shown in Table , the method proposed in the current study offered certain advantages: 1) the range of baicalin concentrations that could be detected was greater than that offered by the method; 2) the detection limit of baicalin was lower than those offered by methods; and (c) compared to the method, this method did not require tedious pretreatment.…”

Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis

“…Comparing with the structural data reported in literatures of the components in Honeysuckle [27][28][29][30][31], 7 of the 262 components were identified as quercetin-3-O-␤-d-glucoside (peak 37), luteolin-7-O-␣-d-glucoside (peak 44), chlorogenic acid 4 (peak 46), quercetin (peak 91), 3,5-O-dicaffeoylquinic acid (peak 112), luteolin (peak 116) and caffeic acid, respectively. The analysis of structural information for them obtained from Table 3 Components separated from the extract of Honeysuckle with detection only by UV detector and APCI/MS, respectively…”

“…6f. As reported by Zhao et al [31], caffeic acid was also an active compound in Honeysuckle and the molecular weight was calculated as 180. 16, it was not detected neither by UV detector nor with APCI/MS, but the corresponding Na + /K + adduct ion peak at m/z 202.94 (M + Na) + and m/z 218.95 (M + K) + were characterized by MALDI-TOF/MS analysis as shown in Fig.…”

Separation methods used for Scutellaria baicalensis active components

“…; effective length 40 cm), operated at an applied voltage of 25 kV, with 25 mM borax at pH 8.5 as running buffer and detection at 310 nm. A method was developed for the simultaneous determination of three constituents, baicalin, chlorogenic acid and caffeic acid in seven traditional Chinese medicinal preparations containing Honeysuckle flower and/or Scutellariae radix by capillary zone electrophoresis [88]. The analytes were separated successfully within 3.5 min using 10 mM borate buffer (pH 8.6).…”