Separation methods used for Scutellaria baicalensis active components

Li, Hua-Bin; Jiang, Yue; Chen, Feng

doi:10.1016/j.jchromb.2004.06.045

Cited by 106 publications

(47 citation statements)

References 61 publications

(98 reference statements)

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“…Previous studies have showed that Scutellaria baicalensis Gerogi containing over 30 different kinds of flavonoids (24,52,53), including baicalin, baicalein, oroxylin A and wogonin (53)(54)(55). It was reported that ethanol extracts of Scutellaria baicalensis Gerogi prevent oxidative damage (56,57) and has anti-inflammation (58,59) and anti-angiogenesis effects (60). In addition, Scutellaria baicalensis Gerogi extract triggers G 2 /M arrest and caspase-dependent apoptosis by modulating ERK pathway in HSC-T6 cells (58).…”

Section: Discussionmentioning

confidence: 99%

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

Lin

Tsai

Tseng

et al. 2013

International Journal of Oncology

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Baicalin is one of the major compounds in the traditional Chinese medicinal herb from Scutellaria baicalensis Georgi. We investigated the molecular mechanisms of cell autophagy induced by baicalin in human bladder cancer T24 cells. Baicalin inhibited cell survival as shown by MTT assay and increased cell death by trypan blue exclusion assay in a concentration-dependent manner. Baicalin did not induce apoptotic cell death in T24 cells by TUNEL and caspase-3 activity assay. Baicalin induced the acidic vesicular organelle cell autophagy marker, manifested by acridine orange (AO) and monodansylcadaverine (MDC) staining and cleavage of microtubule-associated protein 1 light chain 3 (LC3). The protein expression levels of the Atg 5, Atg 7, Atg 12, Beclin-1 and LC3-II were upregulated in T24 cells after baicalin treatment. Inhibition of autophagy by 3-methyl-adenine (an inhibitor of class III phosphatidylinositol-3 kinase; 3-MA) reduced the cleavage of LC3 in T24 cells after baicalin treatment. Furthermore, protein expression levels of phospho-AKT (Ser473) and enzyme activity of AKT were downregulated in T24 cells after baicalin treatment. In conclusion, baicalin triggered cell autophagy through the AKT signaling pathway in T24 cells.

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Section: Discussionmentioning

confidence: 99%

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

Lin

Tsai

Tseng

et al. 2013

International Journal of Oncology

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“…In addition, this compound possesses various therapeutic effects for conditions such as obesity, diabetes, inflammation and neurodegenerative disease [50-53]. Scutellaria baicalensis has been used to treat inflammation, cancer, bacterial and viral infections of the respiratory and gastrointestinal tracts [54-56]. Some of the components of GJHT are reported to possess anti-cancer effects for various types of malignancy via distinct molecular mechanisms and include Raphanus sativus [57,58], Astragalus membranaceus [59-61], Poria cocos [62], Citrus unshiu [63], Platycodon grandiflorum [64] and Anthriscus sylvestris [65].…”

Section: Discussionmentioning

confidence: 99%

The effects of Gamijinhae-tang on elastase/lipopolysaccharide-induced lung inflammation in an animal model of acute lung injury

Sohn

Jang

Kim

et al. 2013

BMC Complement Altern Med

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BackgroundGamijinhae-tang (GJHT) has long been used in Korea to treat respiratory diseases. The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity. However, the precise mechanisms underlying its effects are unknown. This study was conducted to evaluate the protective effects of GJHT in a porcine pancreatic elastase (PPE) and lipopolysaccharide(LPS) induced animal model of acute lung injury (ALI).MethodsIn this study, mice were intranasally exposed to PPE and LPS for 4 weeks to induce chronic obstructive pulmonary disease (COPD)-like lung inflammation. Two hours prior to PPE and LPS administration, the treatment group was administered GJHT extracts via an oral injection. The numbers of neutrophils, lymphocytes, macrophages and total cells in the bronchoalveolar lavage (BAL) fluid were counted, and pro-inflammatory cytokines were also measured. For histologic analysis, hematoxylin and eosin (H&E) stains and periodic acid-Schiff (PAS) stains were evaluated.ResultsAfter inducing ALI by treating mice with PPE and LPS for 4 weeks, the numbers of neutrophils, lymphocytes and total cells were significantly lower in the GJHT group than in the ALI group. In addition, the IL-1β and IL-6 levels were significantly decreased in the GJHT group. The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.ConclusionsThe results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation. The remarkable protective effects of GJHT suggest its therapeutic potential in COPD treatment.

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“…Flavonoids such as baicalin, wogonoside, wogonin, and oroxylin A are the main constituents in the radix of this plant, and more than 30 flavonoids have been identified up till now [3]. The extract of S. baicalensis has been reported to possess a variety of biological effects including anti-oxidant, anti-inflammatory, anti-bacterial, anti-tumor, and anti-diabetic activities [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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Separation methods used for Scutellaria baicalensis active components

Cited by 106 publications

References 61 publications

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

The effects of Gamijinhae-tang on elastase/lipopolysaccharide-induced lung inflammation in an animal model of acute lung injury

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

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