Determination of Amikacin Isomers by High Pressure Liquid Chromatography

Maitra, Shyamal K.; Yoshikawa, Thomas T.; Steyn, Catherine M.; Guze, Lucien B.; Schotz, Michael C.

doi:10.1080/01483917908060106

Cited by 9 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amikacin isomers were first adsorbed on a silica gel column before reaction with OPA; the derivatives were eluted with ethanol before separation by reversed-phase HPLC. (173) A comparison of pre-and postcolumn OPA reaction conditions for gentamicin has been made. (174) For all these OPA fluorescence methods, detection limits are about 1 µg mL −1 .…”

Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

show abstract

Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Most LC methods for the determination of amikacin in various samples required the use of different derivatization reactions to provide adequate detection. First LC analysis of aminoglycosides was involving either pre-or post-column derivatization with o-phthalaldehyde (OPA) [6][7][8] or pre-column derivatization with 1-fluoro-2,4-dinitrobenzene [9], 2,4,6-trinitrobenzene sulphonic acid [10], 1-naphtoyl chloride [11], 1-naphtyl isothiocyanate [12] or 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate [13] prior to UV detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of amikacin in cerebrospinal fluid by high-performance liquid chromatography with pulsed electrochemical detection

Brajanoski

Hoogmartens

Allegaert

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

“…However, the HPLC of amikacin is not straightforward because the absence of chromogenic or fluorogenic groups in its molecule necessitates the use of different derivatization reactions to provide adequate detection. Thus, pre-and post-column derivatization with o-phthalaldehyde (OPA) [5][6][7] and pre-column derivatization with 1-fluoro-2,4-dinitrobenzene [8], 2,4,6-trinitrobenzenesulfonic acid [9], 1-naphthoyl chloride [10], 1-naphthyl isothiocyanate [11], 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate [12], etc., have also been described. However, these techniques are time-consuming and give problems with quantitation.…”

Section: Introductionmentioning

confidence: 99%