Determination of Alkaloids inCatharanthus roseusandVinca minorby High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Abstract

Downloaded by [University of Nebraska, Lincoln] at 04:33 30 December 2015 2 Many plants in the family Apocynaceae have been used for medicinal purposes. Catharanthus roseus and Vinca minor are the most important medicinal plants that belong to the Apocynaceae family. A procedure is reported for the determination of the bioactive alkaloids tabersonine, serpentine, vindoline, catharanthine, tryptamine, and vincamine in Apocynaceae plants (C. roseus and V. minor) by liquid chromatography electrospray ionization tandem mass spectrometry. Quantitation was performed from positive multiple reactions at 161.4  144.2, 349.4  263.0, 457.6  188.0, 337.5  168.3, 337.4  144.2, 355.4  337.2 and 854.5  286.4 for tryptamine, serpentine, vindoline, tabersonine, catharanthine, vincamine, and paclitaxel (the internal standard), respectively. The optimized method was employed for the determination of the active alkaloids in the leaves of C. roseus and V. minor by liquid chromatography -tandem mass spectrometry.

Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

“…In total 72 TIAs were identified of which 11 were characterized unambiguously while 61 were assigned tentatively. The present method was shorter and more informative than previously reported methods …”

“…All the TIAs were analyzed in positive electrospray ionization (+ESI) mode . The base peak chromatograms (BPCs) of the C. roseus samples are presented in Figure S3 (supporting information): A–E (leaf), F–J (stem) and K–O (root) of C. roseus , in Figures S3A, S3B and S3C, respectively, collected from the states of Assam, Uttar Pradesh, Arunachal Pradesh, Jammu and Kashmir, and Bihar.…”

“…All the TIAs were analyzed in positive electrospray ionization (+ESI) mode. 35,36,[38][39][40][44][45][46][47] The base peak chromatograms (BPCs) of the C. roseus samples are presented in Figure S3 44,47 In the present study nine classes of TIAs (vinpocetine, yohimbine, vindesine, ajmaline, ajmalicine, serpentine, catharanthine, vinblastine, vindoline, vincristine and reserpine) were selected as templates for screening of their classes of compounds. The fragmentation pathways for vinpocetine, vindesine, catharanthine, vinblastine, vindoline and vincristine were established.…”

“…The volatile and semi‐volatile compounds in plant parts of C. roseus have been identified by GC/MS where the non‐volatile compounds were derivatized prior to analysis . Vincristine, vinblastine, 19‐ S ‐vindoline, vindolinine, ajmalicine and an ajmalicine isomer, tabersonine, catharanthine, serpentine and a serpentine isomer were identified in crude extracts of C. roseus using direct infusion into an ESI source and HPLC/DAD/ESI‐MS/MS . To date, MS or LC/MS/MS analysis on C. roseus has only focused on a few compounds from the leaves/stem of C. roseus …”

Gas Chromatography-Mass Spectroscopy Studies on Ethanolic Extract of Dried Leaves of Catharanthus Roseus

“…Leaves are used in the treatment of menorrhagia, rheumatism, dyspepsia, indigestion, dysmenorrhea, diabetes, hypertension, menstrual disorders, antiallergic, anti-inflammatory, antimicrobial, antithrombotic, cardioprotective, antihelminthic, hypolipidemic, skin diseases, bleeding diarrhea, and antiviral properties. Currently, herbal research has been mainly focusing on isolation, characterization, identification, and quantification of bioactive constituents and secondary metabolites [7][8][9][10][11][12][13]. Gas chromatography-mass spectrometry (GC-MS) is one such sophisticated analytical technique used in identification, detection, and analysis of the constituents.…”