Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of <scp><i>Catharanthus roseus</i></scp> using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Kumar, Sunil; Bajpai, Vikas; Singh, Awantika; Kumar, Brijesh

doi:10.1002/rcm.8037

Cited by 13 publications

(4 citation statements)

References 52 publications

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“…The fragmentation pathways of indole alkaloids are summarised as follows (Kumar et al. 2016 , 2018 ): (1) If a double bond existed between C-3 and C-14, no mass fragments of ring split were found because a stable conjugation structure was formed by π-bonds. If not, a fragment ion from reverse the Diels-Alder split of ring-D would be discovered.…”

Section: Resultsmentioning

confidence: 99%

Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol

Zhang

Yin

et al. 2022

Pharmaceutical Biology

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Context Zuojin Pill (ZJP) has been used to treat gastrointestinal problems in China for hundreds of years. Objective To discover more potential active ingredients and evaluate the gastroprotective mechanisms of ZJP. Materials and methods An approach involving UPLC-Q-Orbitrap HRMS and serum pharmacochemistry was established to screen the multiple chemical constituents of ZJP. Male Sprague-Dawley (SD) rats were divided into six groups: normal control, ulcer control, omeprazole (30 mg/kg), and three ZJP groups (1.0, 2.0 and 4.0 g/kg). After oral treatment with ZJP or omeprazole for 7 days, all groups except the normal control group were orally administered 5 mL/kg ethanol to induce gastric ulceration. Histopathological assessment of gastric tissue was performed by haematoxylin and eosin staining. Antioxidant parameters and inflammatory mediators were determined using ELISA Kit and immunohistochemical analysis. Results Ninety components were identified in ZJP. Among them, 23 prototypes were found in rat serum after oral administration of ZJP. The ulcer inhibition was over 90.0% for all the ZJP groups. Compared with the ulcer control rats, ZJP (4.0 g/kg) enhanced the antioxidant capacity of gastric tissue: superoxide dismutase (1.33-fold), catalase (2.61-fold), glutathione (2.14-fold), and reduced the malondialdehyde level (0.48-fold). Simultaneously, the ZJP meaningfully lowered the content of tumour necrosis factor-α (0.76-fold), interleukin-6 (0.66-fold), myeloperoxidase (0.21-fold), and nuclear factor kappa B (p65) (0.62-fold). Discussion and conclusions This study showed ZJP could mitigate ethanol-induced rat gastric ulcers, which might benefit from the synergistic actions of multiple ingredients. The findings could support the quality control and clinical trials of ZJP.

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Section: Resultsmentioning

confidence: 99%

Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol

Zhang

Yin

et al. 2022

Pharmaceutical Biology

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“…Terpenes were also the main VOCs present in ACC, followed by esters. According to Kumar et al [ 21 ], terpenes are generally considered to be active constituents in natural products that exhibit antibacterial, antitumor, anti-wrinkle, antioxidative, anti-tussive, analgesia, and immune-regulatory effects.…”

Section: Discussionmentioning

confidence: 99%

Honeycomb, a New Food Resource with Health Care Functions: The Difference of Volatile Compounds found in Apis cerana and A. mellifera Honeycombs

Guo

Liang

et al. 2022

Foods

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The honeycomb composition is very complex, containing honey, royal jelly, pollen, and propolis, and thus contains a large number of bioactive ingredients, such as polyphenols and flavonoids. In recent years, honeycomb as a new functional food resource has been favored by many bee product companies, but the basic research on honeycomb is lacking. The aim of this study is to reveal the chemical differences between A. cerana honeycombs (ACC) and A. mellifera honeycombs (AMC). In this paper, we studied the volatile organic components (VOCs) of ACC and AMC by solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS). A total of 114 VOCs were identified in 10 honeycombs. Furthermore, principal component analysis (PCA) revealed that the chemical composition of ACC and AMC were different. Additionally, orthogonal partial least squares discrimination analysis (OPLS-DA) revealed that benzaldehyde, octanal, limonene, ocimene, linalool, α-terpineol, and decanal are the significant VOCs in AMC extracts, which are mainly derived from propolis. OPLS-DA model also identified 2-phenylethanol, phenethyl acetate, isophorone, 4-oxoisophorone, betula, ethyl phenylacetate, ethyl palmitate, and dihydrooxophorone as potential discriminatory markers of ACC, which likely contribute to protecting the hive against microorganisms and keep it clean.

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“…The most abundant terpenes (Table 2) in SD, of which the concentrations were above 1%, were linalool (2.37-3.36%), aromadendrene (3.70-4.80%), zingerone (1.51-5.48%), cedrol (4.66-6.97%), anethol (0.56-12.78%), and α-cadinol (1.15-2.21%). The terpenes were generally considered to be active constituents in natural products [27], which exhibited antibacterial, antitumor, anti-wrinkle, antioxidative, anti-tussive, analgesia, and immune-regulatory effects [28]. For example, Gunaseelan [29] and others found the treatment of linalool prevented acute ultraviolet-B-induced hyperplasia, lipid peroxidation, and anti-oxidation loss of mice skin and could further inhibited overexpression of cyclooxygenase-2 and ornithine decarboxylase in mice skin.…”

Section: Fingerprints Of Selaginella Doederleiniimentioning

confidence: 99%

Analysis of the Volatile Components in Selaginella doederleinii by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

Zhang

et al. 2019

Molecules

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Selaginella doederleinii (SD) is a perennial medicinal herb widely distributed in China. In this study, the volatile components of SD from two regions (24 batches), namely Zhejiang and Guizhou, were determined by combining headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS). After investigating different influence factors, the optimal conditions for extraction were as follows: The sample amount of 1 g, the polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber of 65 µm, the extraction time of 20 min, and the extraction temperature of 100 °C. Based on the above optimum conditions, 58 volatiles compounds, including 20 terpenes, 11 alkanes, 3 alcohols, 6 ketones, 3 esters, 11 aldehydes, 1 ether, 1 aromatic, 1 phenol, and 1 furan, were found and identified in SD. Furthermore, hierarchical cluster analysis and principal component analysis were successfully applied to distinguish the chemical constituents of SD from two regions. Additionally, anethol, zingerone, 2,4-di-tert-butylphenol, ledene, hexyl hexanoate, α-cadinol, phytone, hinesol, decanal, octadecene, cedren, 7-tetradecene, copaene, β-humulene, 2-butyl-2-octenal, tetradecane, cedrol, calacorene, 6-dodecanone, β-caryophyllene, 4-oxoisophorone, γ-nonanolactone, 2-pentylfuran, 1,2-epoxyhexadecane, carvacrol, n-pentadecane, diisobutyl phthalate, farnesene, n-heptadecane, linalool, 1-octen-3-ol, phytane, and β-asarone were selected as the potential markers for discriminating SD from 24 habitats in Zhejiang and Guizhou by partial least squares discrimination analysis (PLS-DA). This study revealed the differences in the components of SD from different regions, which could provide a reference for the future quality evaluation.

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Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Abstract: The developed analytical method together with diagnostic fragment patterns were used to rapidly and effectively identify targeted and untargeted TIAs in C. roseus. A PCA study of the results obtained was used to discriminate among the C. roseus samples.

Cited by 13 publications

References 52 publications

Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol

Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol

Honeycomb, a New Food Resource with Health Care Functions: The Difference of Volatile Compounds found in Apis cerana and A. mellifera Honeycombs

Analysis of the Volatile Components in Selaginella doederleinii by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

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