Gas Chromatography-Mass Spectroscopy Studies on Ethanolic Extract of Dried Leaves of Catharanthus Roseus

Abstract

Objective: Phytochemical screening of the ethanolic extract of Catharanthus roseus disclosed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, flavonoids, and proteins. In the present research work, we have identified and confirmed the structures of the constituents present by means of a hyphenated technique of gas chromatography-mass spectrometry (GC-MS) from the extract.Method: Shade-dried leaves of C. roseus were powdered and extracted by means of Soxhlet extraction using ethanol as a solvent. Crude extract obtained was utilized for GC-MS. Results:A total of 15 components were identified, some of which were n-hexadecanoic acid, ethyl ester, Vitamin E, 9,12,15-octadecatrienoic acid,2-[(trimethylsilyl)oxy]-1-[{(trimethylsilyl)oxy}methyl]ethyl ester, phytol, 3-epivindolinine, and oleic acid. Conclusion:GC-MS studies helped us to assess the phytochemical constituents based on retention time, molecular formula, molecular weight and the corroboration of MS libraries. In future, C. roseus leaves may be subjected to diverse types of extraction methodologies, and a number of various elucidated phytoconstituents can be studied for their ethnopharmacological significance and applicability citing literature.

Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS

“…Phytochemical screening of the methanol extract of C. roseus disclosed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, flavonoids, and proteins. Identified and confirmed the structures of the constituents present utilizing a hyphenated technique of gas chromatography-mass spectrometry (GC-MS) from the extract (Doshi et al 2018). The most attractive values of C. roseus can produce more than 130 kinds of terpenoid indole alkaloids (TIAs) (Chen et al 2017).…”

“…The presence of phytochemical components such as alkaloids and flavonoids of C. roseus leaves are developing resistance against many of the currently available antimicrobial drugs (Patil and Ghosh 2010). Phytochemical screening of the ethanolic extract of C. roseus disclosed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, flavonoids, and proteins (Doshi et al 2018). C. roseus cultivar Milkey white and Pink could efficiently be used in the development of new life-saving drugs.…”

Determination of Vindoline and Rutin Content in Five Different Morphotypes of Catharanthus Roseus Leaves Using HPLC

“…Catharanthus roseus, commonly known as periwinkle belongs to the family Apocynaceae. It grows naturally as a wildflower throughout the sub-tropical region like Southern Europe, Australia, Africa, America and Asia [4]. This plant is broadly investigated due to its essential indole alkaloids like vindoline and catharanthine.…”