Determination of aliphatic amines by cation‐exchange chromatography with suppressed conductivity detection after solid phase extraction*

Abstract

A sensitive method for the determination of underivatized aliphatic amines based on cation exchange chromatography coupled with suppressed conductivity detection scheme and solid phase extraction (SPE) procedure has been developed. A surface modified styrene divinylbenzene polymeric sorbent, based on a reversed-phase (RP) and strong cation exchange (SCX) mixed mode was used as an active material for the SPE of amines. The conductometric capabilities of several aliphatic monoand polyamines, expressed in terms of molar sensitivity (nS/lM), were determined. The LODs, obtained without the SPE treatment, ranged between 20 and 65 nM for putrescine and 2-butylamine, respectively. The resulting calibration plots for the aliphatic amines were generally linear over about three orders of magnitude, with correlation coefficients A0.98. The LODs of amines decreased generally by one factor when SPE procedure, using BaCl 2 /H 2 SO 4 eluents, has been adopted. The proposed SPE procedure, seems to offer good results in terms of preconcentration, recoveries and cleanup of samples. The proposed methodology was successfully tested for the quantitative determination of some biogenic amines in beer and tuna.

Aliphatic Polyamine Oxidation Reaction Mechanism at Boron‐doped Microcrystalline and Ultrananocrystalline Diamond Electrodes

“…Because of their role in cell proliferation, polyamines are also involved in carcinogenesis, stimulating research into polyamine metabolism as a target in cancer therapy 2. Polyamines accumulate in foods and beverages as a direct consequence of bacterial enzymatic processes 4–6. The analytical determination of these polyamines is important because they can be a direct indicator of microbial contamination 4–6.…”

“…Polyamines accumulate in foods and beverages as a direct consequence of bacterial enzymatic processes 4–6. The analytical determination of these polyamines is important because they can be a direct indicator of microbial contamination 4–6. Typically, these molecules are detected by gas or liquid chromatography after derivatization and often in combination with solid phase extraction 7–9.…”

“…Typically, these molecules are detected by gas or liquid chromatography after derivatization and often in combination with solid phase extraction 7–9. Cation exchange chromatography of underivatized aliphatic amines has also been reported 4. Suppressed conductivity detection and solid phase extraction were used to achieve limits of detection in the 20–70 nM range, depending on the polyamine 4.…”

Study of the naphthalene-2,3-dicarboxaldehyde pre-column derivatization of biogenic mono- and diamines in mixture and fluorescence−HPLC determination

“…The developed methods employ mainly separation techniques such as thin-layer chromatography [16], gas chromatography [17,18], capillary electrophoresis [19][20][21][22][23], ion exchange chromatography [24][25][26] and high-performance liquid chromatography (HPLC), which is the most widely used technique [27][28][29][30][31][32][33].…”

Chromatographic Methods for the Determination of Polyfunctional Amines and Related Compounds Used as Monomers and Additives in Food Packaging Materials: A State‐of‐the‐Art Review

“…Casella and others (2008) determined the amines without previous derivatization by using cation‐exchange chromatography with suppressed conductivity detection. The underivatized amines were separated on an Ion Pac CS17 (250 × 4 mm) cation‐exchange column (macroporous 7 μm 55% cross‐linked poly(ethylvinylbenzene‐divinylbenzene) in gradient elution mode and using 50 mM H 2 SO 4 and water as the mobile phase.…”