Study of the naphthalene-2,3-dicarboxaldehyde pre-column derivatization of biogenic mono- and diamines in mixture and fluorescence−HPLC determination

Abstract

A new fluorescence-HPLC method was developed for the simultaneous determination of eight biogenic monoamines (histamine, methylamine, tyramine, ethylamine, propylamine, tryptamine, 2-phenylethylamine, isoamylamine) and two biogenic diamines (putrescine, cadaverine) in the presence of heptylamine as the internal standard. The amines were pre-column derivatized with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion as the nucleophile. The effect of the derivatization reaction conditions on the reaction yield was investigated. The derivatives were separated on an Inertsil ODS-3 column (250 × 4mm i.d., 5 μm) using gradient elution and detected fluorimetrically at excitation and emission wavelengths of 424 and 494 nm, respectively. Limits of detection between 0.002 and 0.4 ng, injected on-column (10-μL loop), were achieved. The within- and between-day relative standard deviations ranged between 0.2-3.4% and 0.3-4.8%, respectively. The utility of the method in assaying biogenic mono- and diamine mixtures in Greek cheeses is demonstrated. Ultrasound-assisted liquid-liquid extraction was applied prior to derivatization.

Study of metabolic activity of human embryos focused on amino acids by capillary electrophoresis with light‐emitting diode‐induced fluorescence detection

“…The maximum derivatization yields were obtained when 2.5 mM NDA and 2.5 mM NaCN were used. A decrease in derivatization yields was observed with higher concentrations of NaCN, which is in agreement with the literature .…”

Novel approaches for the determination of biogenic amines in food samples

“…Previous published works reported that NDA could be dissolved both in acetonitrile or methanol, without detailing if a contribution of the solvent in the derivatizing reaction is present [17,32].…”

Study on the Binding Interaction of the α,α′,δ,δ′-Tetramethylcucurbit[6]uril With Biogenic Amines in Solution and the Solid State

“…Numerous methods, such as chromatography (LC, HPLC, GC, and ion exchange), electrophoretic techniques, fluorescence, and UV–Vis spectroscopy (Khuhawar and Qureshi, 2001 ; Reinemann et al, 2009 ; Zotou and Notou, 2012 ; Mohammed et al, 2016 ; Sun et al, 2017 ), have been reported for BA detection. Furthermore, based on selective recognition through non-covalent interactions between a receptor unit and an analyte, the supramolecular-complex method has been used to identify BAs.…”