Determination of 25 Low Molecular Weight Carbohydrates in Tobacco by High Performance Ion Chromatography

Ramusino, MC; Dattilo, BS; Lucibello, A; Rossi, SG

doi:10.2478/cttr-2013-0634

Cited by 4 publications

(4 citation statements)

References 15 publications

(8 reference statements)

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“…In general, tobacco varieties with high sugar contents are considered to be, of better quality [31]. Leaf glucose content in oriental tobacco leaves was reported to be 2.0% [32], 2.98% [27], and 4.2% [28]. The sugar content values for oriental-type varieties, in the present study, were higher than those reported in the previous referred studies and ranged from 2.71 to 9.78% (Supplementary Table S15).…”

Section: Discussioncontrasting

confidence: 46%

Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions οf Greece

Tsaliki,

Moysiadis,

Toumpas

et al. 2023

Agriculture

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Tobacco (Nicotiana tabacum L.) is an important industrial crop grown in more than one hundred countries worldwide, with high adaptability on a great variety of soils, and under quite diverse climatic conditions. Information regarding the chemical composition of tobacco leaves is important for the tobacco industry because it generally may exhibit important differences among different locations. In this study, five oriental and six flue and air-cured type tobacco varieties were cultivated in five areas of Greece for three cultivation periods, in order to evaluate the effect of these three factors (variety, area, and period), on the yield as well as nicotine content, and sugar and nitrate content of the produced leaves. For the oriental type varieties, the results showed that the area exhibited significant interaction with both variety and the cultivation year, related to the yield. Concerning the nicotine content, no significant differences were observed regarding the areas of experimentation; however, it significantly varied within the varieties. In particular, the nicotine content of Katerini 53 exhibited a significant difference compared to Xanthi 2A, which had the highest content of all tested varieties. Sugar content was affected significantly by all the factors considered, with the area of Xanthi exhibiting lower values compared to all other varieties. Regarding the flue and air-cured varieties, the only interaction that was found to be statistically significant, when the yield was assessed, was between the cultivation year and the area. It was found that there was a significant interaction between cultivation year and area for the nicotine content as well. Furthermore, cultivation year and variety significantly affected the sugar content. The varieties Burley 21E and NC7LC gave the lowest sugars. In general, it was observed that in the case of the oriental type varieties, the variety adaptation in specific regions resulted in higher yields, with the paradigmatic example of the variety Katerini 53 in the Katerini area, and Xanthi 81 in the Xanthi area. On the other hand, in the case of the flue and air-cured varieties, Karditsa was the area that exhibited the lowest yield of all tested varieties.

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Section: Discussioncontrasting

confidence: 46%

Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions οf Greece

Tsaliki,

Moysiadis,

Toumpas

et al. 2023

Agriculture

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“…Glucose and fructose are the most important soluble sugars and are called reducing sugars (Roomer et al, 2012). The glucose ratio in oriental tobacco is reported at least 2% (Ramusino et al, 1994), but studies reporting around 10% were also encountered (Sekin, 1979). The sugar content of the tobaccos grown in Black Sea region is expected to be always contain less sugar than the Izmir type (Sekin, 1979).…”

Section: Variations In Physical and Chemical Characteristicsmentioning

confidence: 99%

Stability Analyses for Interpreting Genotype by Environment Interaction of Selected Oriental Tobacco Landraces

Kurt¹

2020

Turkish Journal of Field Crops

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Advanced pure lines, which are in different genetic and vegetative characteristics determined in a breeding program initiated in 2013, were included to the yield tests in the region before registration. Chemical and morphological variability and stability of 13 advanced pure tobacco lines and 2 standard varieties were determined in two different locations. The experimental layout was random blocks with three replications and the experiment was carried out in 2016 and 2017. Plant height values ranged from 85.25 to 156.71 cm, the number of leaves from 22.43 to 41.71 leaf plant -1 , leaf width ranged from 9.35 to 11.52 cm, leaf length from 17.36 to 21.89 cm, yield from 1371.9 to 1756.7 kg ha -1 , quality grade index from 67.33 to 81.58%, nicotine content from 1.02 to 1.98% and glucose content from 3.33 to 9.33%. The lines 16, 21, 26 and 45 were identified as strong variety candidates suitable for the region based on all the parameters and the statistical conditions examined. The use of biplots that the environments were distributed on axes, enabled to make location-based variety recommendations. The biplot graph based on environment and genotype interaction was used in the recommedations of tobacco variety for each location.

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“…The quantitative analysis of inositol has been reported together with several carbohydrates in tobacco (6), as well as in other materials (7,8). Analysis of quinic acid has also been reported in different materials, such as in tea (9), but quantitative information regarding the level of this compound in tobacco is scarce (4,10).…”

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Analysis of Quinic Acid and of myo-Inositol in Tobacco

Moldoveanu

Davis

2012

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARYQuinic acid [D(-)-quinic acid, (1S, 3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid], and inositol [myoinositol, (1R,2R,3S,4S,5R,6S-cyclohexane-1,2,3,4,5,6-hexol or cis-1,2,3,5-trans-4,6-cyclohexane-hexol] are oxygenated compounds well known as tobacco constituents. Although mentioned in the literature as constituents of tobacco leaf as early as 1930, very little information is offered regarding the quantitation of quinic acid and inositol, up to the present day. This study describes a simple procedure for the analysis of quinic acid and myo-inositol in tobacco and reports the levels of these two analytes in various single grade and blended tobaccos from commercial and Kentucky reference cigarettes. The procedure is based on an original LC/MS/MS analysis of the tobacco extract. This method and its validation are described in this paper. A wide range of levels of the analytes was observed from sample to sample. Burley samples were in general low in both quinic acid and inositol compared to flue-cured tobaccos. The levels of myo-inositol detected in the analyzed samples were consistent with previously reported results for flue-cured tobaccos, but significantly lower than those reported for Burley. Besides myo-inositol, a number of naturally occurring stereoisomers of inositol are known. These include scyllo-, muco-, D-chiro-and neo-inositol. Other isomers are possible, including L-chiro-, allo-, epiand cis-inositol, but they are less common. No previous study has been reported regarding the evaluation of these compounds in tobacco, and the present study indicated that only myo-inositol and D(-)-quinic acid are found in tobacco. [Beitr. Tabakforsch. Int. 25 (2012) 2,3,4,5,6-hexol ou cis-1,2,3,5-trans-4,6-cyclohexanehexol] sont des composés oxygénés bien connus comme constituants du tabac. Bien qu'ils aient été mentionnés dès 1930 comme constituants de la feuille de tabac dans la littérature spécialisée, il n'existe jusqu'à présent que très peu d'informations concernant la

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Determination of 25 Low Molecular Weight Carbohydrates in Tobacco by High Performance Ion Chromatography

Cited by 4 publications

References 15 publications

Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions οf Greece

Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions οf Greece

Stability Analyses for Interpreting Genotype by Environment Interaction of Selected Oriental Tobacco Landraces

Analysis of Quinic Acid and of myo-Inositol in Tobacco

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