The present investigation includes the blood biochemical alterations in chronic (30 days) exposure of the airbreathing fish, Clarias batrachus (Linn.) induced by sublethal concentration (2.46 mg/L) of inorganic herbicide, glyphosate. Significant changes were observed in the blood biochemical properties in the form of hyperglycemia, hypoproteinemia and hypercholesterolemia. The present study therefore points towards a severe metabolic dysfunction in response to herbicide, glyphosate toxicity in the fish Clarias batrachus. So, it is suggested that more suitable to culture at water glyphosate concentration of < 2.46 mg/L for optimum growth performance and survival rate than other water conditions.