Detection, Survival, and Source of Inoculum of Pseudomonas syringae pv. syringae from Weeds and Plant Debris in Relation to Epidemiology of Bacterial Citrus Blast and Black Pit in Tunisia

Mougou, Imen; Boughalleb-M’Hamdi, Naïma

doi:10.9734/bmrj/2016/27954

Cited by 6 publications

(9 citation statements)

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“…P. cerasi (PG02a), P. kairouanensis and P. nabeulensis , should be included in future surveys of pathogens involved in citrus blast and black pit in Tunisia as well as in other countries where these diseases are present. It will be important to include these species in the diagnostic scheme, identification of reservoirs (Mougou and Boughalleb-M'hamdi 2016 ) and the biocontrol research mainly focused on P. syringae (PG02b) (Mougou and Boughalleb-M’hamdi 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Citrus blast has been reported in Turkey (Mirik et al 2005 ), Iran (Beiki et al 2016 ) and Montenegro (Ivanović et al 2017 ). In Tunisia, P. syringae has been described on tomato (Mensi et al 2018 ), on citrus (Abdellatif et al 2015 ) and it was isolated from weeds and plant debris that can be the source of inoculums to trigger citrus blast and black pit diseases (Mougou and Boughalleb-M’Hamdi 2016 ). Given the significant damages observed in the field, the quick emergence of the disease and the economic importance of citrus cultivation in Tunisia, research on these diseases has recently grown.…”

Section: Introductionmentioning

confidence: 99%

“…So far, the polyphagous nature and the diversity of these bacteria as well as its adaptive and resistant capacities have been more or less under estimated in Tunisia, although several studies focused on citrus blast and citrus black pit. Only similar pathogenic P. syringae strains belonging to phylogroup PG02b as described by Berge et al ( 2014 ) were described (Mougou and Boughalleb-M'hamdi 2016 ; Abdellatif et al 2017 ). In Montenegro, strains isolated from necrotic mandarin buds have been identified as one homogenous lineage of P. syringae pv.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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“…Black pit symptoms are visible on citrus fruits [12] Although no citrus cultivar is fully resistant to blast [12], Lemon cultivar 'Eureka' was found least susceptible [13].…”

Section: Citrus Cankermentioning

confidence: 99%

Safeguarding Citrus: Exploring State-of-the-art Management Strategies for Bacterial Citrus Diseases

Kukreti,

Karunakar Reddy

2024

Challenges in Plant Disease Detection and Recent Advancements

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Bacterial diseases pose significant risks to the citrus industry, causing major economic losses and threatening global production. The most severe threats include citrus canker (Xanthomonas citri) which leads to visible lesions on leaves, fruit, and stems and Huanglongbing (HLB) (Candidatus Liberibacter spp.) which results in mottled leaves, stunted growth, and deformed, bitter fruit. Beyond these major diseases, citrus blast and citrus variegated chlorosis (CVC) are emerging concerns. Citrus blast, caused by Pseudomonas syringae pv. citri, results in leaf lesions, cankers, and defoliation, managing it involves copper-based bactericides, removing infected branches, and cultural practices to reduce spread. CVC, caused by Xylella fastidiosa, is a vascular disease leading to chlorosis, leaf scorch, and dieback. Management strategies for CVC include controlling insect vectors with insecticides and developing resistant citrus varieties. Integrated disease management is crucial, focusing on sustainable approaches that combine cultural practices, biological control agents, and resistant varieties. Advances in technology, such as molecular diagnostics, remote sensing, and precision agriculture, are improving early detection and monitoring. Public awareness and education are keys to encouraging growers to adopt best practices. Collaboration among researchers, growers, and policymakers remains essential to tackle the complex challenges of bacterial citrus diseases and ensure the citrus industry’s sustainability.

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“…Cultivars Eureka and Swett Lime seemed to be most susceptible to black pit disease (Mougou and Bougalleb-M'hamdi 2016a). Weeds and plant debris were shown to be a source of P. syringae inoculum (Mougou and Bougalleb-M'hamdi 2016b).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Pseudomonas syringae pv. syringae affecting citrus orchards in Tunisia by using indigenous Bacillus spp. and garlic extract

Mougou

Boughalleb-M’Hamdi

2018

Egypt J Biol Pest Control

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Citrus blast and black pit that became increasingly important bacterial diseases are caused by Pseudomonas syringae pv. syringae. This study aimed to evaluate the antibacterial potential of Bacillus species strains and garlic extracts against two P. syringae isolates (BAT13 and DAPP-PG115). The Bacillus species strains were isolated from symptomless citrus leaves. Under in vitro conditions, 21 Bacillus species strains and garlic extract displayed antibacterial activity against the pathogen. Under greenhouse conditions, antagonistic bacteria, garlic extract, and copper sulfate confirmed their antimicrobial effect on P. syringae and reduced significantly the extend of stem necrosis 10 weeks after inoculation by BAT13 up to 60.55, 56.11, and 45.83%, and by DAPP-PG115 up to 70.83, 62.5, and 46.52%, in respect to relevant treatments. Garlic extract was the most effective treatment in our hands, and it suggests that Allium sativum extract could be used to control and prevent infection by the pathogen.

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Detection, Survival, and Source of Inoculum of Pseudomonas syringae pv. syringae from Weeds and Plant Debris in Relation to Epidemiology of Bacterial Citrus Blast and Black Pit in Tunisia

Cited by 6 publications

References 0 publications

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Safeguarding Citrus: Exploring State-of-the-art Management Strategies for Bacterial Citrus Diseases

Biocontrol of Pseudomonas syringae pv. syringae affecting citrus orchards in Tunisia by using indigenous Bacillus spp. and garlic extract

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