Biocontrol of Pseudomonas syringae pv. syringae affecting citrus orchards in Tunisia by using indigenous Bacillus spp. and garlic extract

Mougou, Imen; Boughalleb-M’Hamdi, Naïma

doi:10.1186/s41938-018-0061-0

Cited by 18 publications

(13 citation statements)

References 36 publications

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“…P. cerasi (PG02a), P. kairouanensis and P. nabeulensis , should be included in future surveys of pathogens involved in citrus blast and black pit in Tunisia as well as in other countries where these diseases are present. It will be important to include these species in the diagnostic scheme, identification of reservoirs (Mougou and Boughalleb-M'hamdi 2016 ) and the biocontrol research mainly focused on P. syringae (PG02b) (Mougou and Boughalleb-M’hamdi 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

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Section: Discussionmentioning

confidence: 99%

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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“…The efficacy of Bacillus isolates in experiments concerning P. syringae biocontrol is evidenced in several recent studies. Bacteria of Brevibacillus genus, were shown to produce non-ribosomal peptide with antimicrobial activity (marthiapeptide A), streptocidin D, and an unusual lysophospholipid, which are active against Gram-negative bacteria (Mougou et al, 2018). Dissimilarities in grain-residing bacterial endophytic communities were also associated with different manifestation of defense reactions in response to grain exposure to phytopathogen in vitro.…”

Section: Dicussionmentioning

confidence: 99%

Beneficial traits of grain-residing endophytic communities in wheat with different sensitivity to Pseudomonas syringae

Pastoshchuk

Yumyna

Zelena

et al. 2021

Regul. Mech. Biosyst.

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Deep insight into compositional and functional features of endophytic bacterial communities residing in wheat grains opens the way to the use of their plant growth promoting and biocontrol abilities in agricultural biotechnology. The aim of this work was to compare grain-residing endophytes from winter wheat varieties with different sensitivity to Pseudomonas syringae pv. atrofaciens (McCulloch) and to examine their plant-beneficial traits and antagonistic effects. Grain-residing bacteria were isolated from surface-sterilized grains of three wheat varieties sown in Ukraine following a culture-dependent protocol, and were screened for their plant growth promotion (PGP) and antagonistic properties. Bacterial morphotypes were represented by gram-negative rods, endospore-forming bacilli and gram-positive cocci. Different resistance to phytopathogenic pseudomonads was associated with distinctive quantitative and functional features of grain-residing endophytic communities. High resistance to P. syringae was coupled with the prevalence of gram-negative rods in the endophytic community, the highest proportion of endophytic bacteria possessing three PGP activities (phosphate solubilization, nitrogen fixation and production of indolic compounds) simultaneously, and with the most potent antagonistic activity of grain-residing endospore-forming bacilli. In total, five grain-residing isolates, which were obtained from three wheat varieties (two isolates from varieties with medium and high resistance and one – from a low-resistant variety), demonstrated ability to restrain P. syringae pv. atrofaciens (McCulloch) growth. Two isolates (P6 and P10) which were obtained from the high-resistant wheat variety Podolyanka and were assigned to Paenibacillus and Brevibacillus genera according to their biochemical profiling and MS-DS identification, showed the most potent antagonistic effects as indicated by maximum inhibition zone in agar well diffusion assay. These results shed light on the association of the features of grain-residing endophytic bacteria with wheat resistance to phytopathogenic pseudomonads. Isolates from the high-resistant wheat variety can be recommended for grain dressing as plant growth promoting and biocontrol agents for P. syringae pv. atrofaciens (McCulloch).

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“…However, the use of chemical bactericides and antibiotics to control phytopathogenic bacteria could cause serious damage to the environment and human health. Moreover, some emerging strains have shown strong resistance to all these products (Sabir et al, 2017;Mougou & Boughalleb-M'hamdi 2018;Wu et al, 2019). Therefore, many researchers have focused on the development of alternative methods of controlling plant diseases.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #3 of "Antimicrobial activity of Streptomyces spp. isolated from Apis dorsata combs against some phytopathogenic bacteria (v0.1)"

Rodrigues¹

2020

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Biocontrol of Pseudomonas syringae pv. syringae affecting citrus orchards in Tunisia by using indigenous Bacillus spp. and garlic extract

Cited by 18 publications

References 36 publications

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Beneficial traits of grain-residing endophytic communities in wheat with different sensitivity to Pseudomonas syringae

Peer Review #3 of "Antimicrobial activity of Streptomyces spp. isolated from Apis dorsata combs against some phytopathogenic bacteria (v0.1)"

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