Beneficial traits of grain-residing endophytic communities in wheat with different sensitivity to Pseudomonas syringae

Pastoshchuk, A. Yu.; Yumyna, Yuliia; Zelena, Pavlina; Nudha, V.; Yanovska, V.; Kovalenko, Mariia; Taran, Nataliya; Patyka, V.P.; Skivka, Larysa

doi:10.15421/022168

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…In the specific case of P. syringae, its biocontrol using fungal endophytes has been demonstrated in various plant species [26,27], including the use of their fungal filtrates [28], which promote plant growth and stimulate plant resistance. Hence, the hypotheses of this study were (1) that the biocontrol activity of a fungal endophyte species (if any) is mainly attributed to the secondary metabolites it produces, and (2) that the filtrates/extracts produced in vitro by this endophyte, which may contain such metabolites, could protect plants against pathogens when applied externally.…”

Section: Introductionmentioning

confidence: 99%

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

García-Latorre,

Rodrigo,

Santamaria

2024

Horticulturae

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Endophytic fungi offer promising alternatives for sustainable plant disease management strategies, often through the production of bioactive secondary metabolites. This study investigated the biocontrol potential of filtrates and extracts, produced under controlled conditions, from Alternaria leptinellae E138 against Pseudomonas syringae in tomato plants under greenhouse conditions. To understand the main mechanisms involved in biocontrol, the direct inhibition of bacterial growth and disruption of quorum sensing activity caused by metabolites were studied in vitro, as well as indirect mechanisms, such as their capacity to produce phytohormone-like substances, nutrient mobilization, and antioxidant activity, which can enhance plant growth and fitness. Moreover, a mass spectrometry analysis was used to tentatively identify the secondary metabolites present in the extract with antimicrobial properties, which could explain the biocontrol effects observed. Mycopriming assays, involving the direct treatment of tomato seeds with the fungal A. leptinellae E138 extracts, produced increased germination rates and seedling vigor in tomato seeds. As another treatment, postemergence application of the extracts in greenhouse conditions significantly improved plant health and resulted in a 41% decrease in disease severity. Overall, this study underscores the potential of A. leptinellae E138 extract as a plant growth promoter with biocontrol capabilities, offering promising avenues for sustainable plant disease management.

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Section: Introductionmentioning

confidence: 99%

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

García-Latorre,

Rodrigo,

Santamaria

2024

Horticulturae

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GRAIN-RESIDING ENDOPHYTIC BACTERIUM Paenibacillus polymyxa P 6.3 POSSESSES GROWTH-PROMOTING ACTIVITY AND PROTECT WHEAT GRAIN FROM PATHOGENIC EFFECT OF Pseudomonas syringae

Shustyk¹

2022

Biotechnol. acta

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Aim. To examine the effect of endophytic bacteria Paenibacillus polymyxa P 6.3, which was isolated from grains of winter wheat variety Podolyanka, on the germination of wheat grains after the exposure Pseudomonas syringae. Methods. Growth-promoting and biocontrol activity of P. polymyxa P 6.3 were examined using roll method. Standardized wheat grains were soaked in a suspension of 24 h culture of P. polymyxa P 6.3 for 12 h, control grains were soaked in sterile distilled H2O. After soaking, both pre-treated and control grains (of 25 pcs) were put into rolls. In three days, half of both pre-treated and control grains were exposed to phytopathogen P. sуringae, and germination was continued. The lengths of coleoptile and main root were measured on the 7th day of the experiment. Results were expressed as M ± m. Differences were considered significant at P ≤ 0.05. Results. Treatment wheat grains with P. polymyxa P 6.3 resulted in increased growth of coleoptile and main root in all three varieties. Most prominent effect was registered in Favorytka variety. After the exposure to phytopathogenic pseudomonads, slowing down of the growth of coleoptile and main root occurred in all wheat varieties. Highest susceptibility to P. syringae pathogenic effect was registered in Holikovska variety. Pre-treatment of wheat grains with endophytic bacteria abrogated growth-inhibiting effects of P.syringae. Conclusion. Endophytic bacteria P. polymyxa P 6.3 exerts a growth-stimulating effect on wheat germination and a protective effect against P. syringae. The plant growth promoting potential and antagonistic activity make strain P 6.3 a promising biocontrol agent and growth stimulator as a biofertilizer.

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Antimicrobial activity of exometabolites of Paenibacillus polymyxa, isolated from endophytic community of winter wheat grain

Pastoshchuk¹,

Shustyk²,

Zelena³

et al. 2022

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursìv ì priro

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In preliminary studies, a strain Paenibacillus polymyxa P6 possessing multiple plant growth-promoting (phosphate solubilization, oligonitrotrophy and production of heteroauxins) and antagonistic activities was isolated from grainresided cultivable bacterial endophytic community of winter wheat variety of the domestic selection Podolyanka with increased resistance to the causative agent of basal bacteriosis of grain crops Pseudomonas syringae pv. atrofaciens. The aim of this work was to study the antimicrobial effect of the exometabolites, produced by isolated strain, towards gram-negative phytopathogenic microorganisms using the method of deferred antagonism under the condition of cultivating the antagonistsc bacterium for 72 and 120 hours before inoculation of test cultures. Microorganisms stored in the collection of the D.K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine: Ralstonia solanocearum B-1109, Pectobacterium carotovora subsp. carotovora B-1077, Pseudomonas syringae pv. syringae B-1022, Pseudomonas syringae pv. syringae van Hall 1902 B-1027, Pseudomonas syringae pv. atrofaciens B-1011, Pseudomonas syringae pv. atrofaciens B-1013, as well as Erwinia amylovora ATCC 15580 were used as a test-cultures. Paenibacillus polymyxa P6 exometabolites were found to have a dose-dependent antimicrobial effect towards all studied microorganisms. The most pronounced inhibitory effect was registered against Erwinia amylovora ATCC 15580, Pseudomonas syringae pv. syringae B1022 and Ralstonia solanocearum B-1109: the diameter of the zone of no growth under the action of exometabolites produced for 120 hours are 25.72 ± 4.0 mm, 22.93 ± 2.0 mm and 20.30 ± 4.0 respectively. These results substantiate the expediency of further investigation of the composition and biological activity of the studied exometabolites in the perspective of developing biotechnological preparations.

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Beneficial traits of grain-residing endophytic communities in wheat with different sensitivity to Pseudomonas syringae

Cited by 3 publications

References 37 publications

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

GRAIN-RESIDING ENDOPHYTIC BACTERIUM Paenibacillus polymyxa P 6.3 POSSESSES GROWTH-PROMOTING ACTIVITY AND PROTECT WHEAT GRAIN FROM PATHOGENIC EFFECT OF Pseudomonas syringae

Antimicrobial activity of exometabolites of Paenibacillus polymyxa, isolated from endophytic community of winter wheat grain

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