Detection, pathogenesis, and therapy of respiratory syncytial virus infections.

Welliver, R C

doi:10.1128/cmr.1.1.27-39.1988

Cited by 13 publications

(28 citation statements)

References 43 publications

(57 reference statements)

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“…Methods are described for the detection of HRSV subtypes using the polymerase chain reaction (Cane and Pringle, 1992). However, the available immunoassays (Welliver, 1988) are based on whole virus or complete proteins that do not discriminate between subtypes of HRSV nor between different RSV types.…”

Section: Introductionmentioning

confidence: 99%

Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

Langedijk

Middel

Schaaper³

et al. 1996

Journal of Immunological Methods

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Peptides deduced from the central hydrophobic region (residues 158-189) of the G protein of bovine and ovine respiratory syncytial virus (RSV) and of human RSV subtypes A and B were synthesized. These peptides were used to develop ELISAs to measure specifically antibodies against these types and subtypes of RSV. We have evaluated the bovine RSV-G peptide in both an indirect ELISA and in a blocking ELISA. Specificity and sensitivity, relative to a routine diagnostic ELISA that detects antibodies against the RSV F-protein in bovine sera, were 98% and 92% respectively for the indirect peptide-based ELISA, and 98% and 98% for the blocking peptide-based ELISA. In paired serum samples, rises in antibody titer were detected more frequently with the indirect peptide-based ELISA than with the routine F-ELISA. Furthermore, the peptide-based G-ELISAs were able to differentiate between antibodies against BRSV and HRSV, and between those against BRSV and ORSV. In addition, the indirect peptide-based ELISA was selective for HRSV subtype A and B antibodies. This study shows that peptides, corresponding to the central hydrophobic region of the attachment protein G of several RSVs, can be used successfully as antigens in highly specific and sensitive immunoassays.

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Section: Introductionmentioning

confidence: 99%

Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

Langedijk

Middel

Schaaper³

et al. 1996

Journal of Immunological Methods

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show abstract

“…All 14 patients with acute and convalescent titers had a confirmatory fourfold rise in antibody against RSV. The remaining 20 of the 34 tested patients had an antibody titer ≥1: 2,084 for RSV, and this was felt by the authors to indicate probable recent infection with RSV because titers in that range are not seen in the general population with remote infection by RSV and a single elevated titer has been used in other studies as a diagnostic test 6 , 7 . Thus, all 34 tested patients were thought to have recent RSV infection.…”

Section: Resultsmentioning

confidence: 98%

An Outbreak of an Influenza‐Like Illness in a Nursing Home

Osterweil¹,

Norman²

1990

J American Geriatrics Society

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As part of an ongoing infection surveillance program, data were collected on an outbreak of respiratory illness affecting 29% of residents in a 231-bed long-term care facility. Viral titers could be collected on 60% of the ill residents and respiratory syncytial virus (RSV) was established in 14 of the symptomatic patients and was thought to be the probable cause of the outbreak. Compared with previously reported long-term institutional outbreaks of RSV, there were significantly fewer cases of pneumonia (5% vs. 14% to 55%) and deaths (2% vs. 6% to 20%). However, morbidity was substantial in this outbreak, with 46% of patients suffering prolonged malaise and anorexia; the mean duration of symptoms was four weeks. Respiratory syncytial virus may be an important cause of respiratory illness in nursing homes and may be unappreciated if specific diagnostic methods are not employed. The recent availability of rapid diagnostic tests and specific antiviral therapy for RSV warrant further clinical studies to develop management strategies for this infection.

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“…RSV infection is a major cause of hospitalization of infants and young children in the Western world (14) and imposes a substantial burden on hospital beds and isolation resources during each winter-spring epidemic. The risk of severe RSV infection is dependent on a multitude of factors including age, host immunity and underlying respiratory or cardiac disease, as well as route and duration of exposure (15).…”

Section: Discussionmentioning

confidence: 99%

“…A common diagnostic method for RSV disease is direct detection of the antigen by ELISA or immunofluorescence assay ( 15). The commercially available direct detection assays use type common antibodies which do not allow distinction of subgroups A and B.…”

Section: Discussionmentioning

confidence: 99%

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Pediatric RSV Infection During Two Winter Seasons in British Columbia: A Role for Subgroup Analysis in Young Children?

Wilson

Örvell

Morrison

et al. 1990

Canadian Journal of Infectious Diseases

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Using a panel of eight monoclonal antibodies directed against the G, F and NP proteins of respiratory syncytial virus, 167 virus isolates from nasopharyngeal washing cultures at British Columbia Children's Hospital during two consecutive epidemics were subgrouped. Slides made and frozen at the time of virus isolation or prepared from recovered frozen passage material, were assayed by indirect immunofluorescence. Of 85 strains tested in 1987-88, 54 (64%) were subgroup A, and 31 (36%) subgroup B. By contrast, of 82 strains tested in 1988-89 five (6%) were subgroup A and 77 (94%) subgroup B. Review of patient charts did not reveal significant differences in clinical course of patients infected with the two subgroups, but the risk of infection with subgroup A was significantly greater than the risk of subgroup B infection in younger patients.

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Detection, pathogenesis, and therapy of respiratory syncytial virus infections.

Cited by 13 publications

References 43 publications

Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

An Outbreak of an Influenza‐Like Illness in a Nursing Home

Pediatric RSV Infection During Two Winter Seasons in British Columbia: A Role for Subgroup Analysis in Young Children?

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