Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

Langedijk, J.P.M.; Middel, W.G.J.; Schaaper, W.M.M.; Meloen, Rob H.; Kramps, J. A.; Brandenburg, A. H.; Oirschot, J.T. van

doi:10.1016/0022-1759(96)00039-7

Cited by 38 publications

(38 citation statements)

References 18 publications

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“…Serum samples collected on days 0, 7 and 14 were tested for the presence of virus-neutralizing antibodies in a virus neutralization assay (Langedijk et al, 1996).…”

Section: Methodsmentioning

confidence: 99%

Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence

Antonis

Jong

Poel

et al. 2010

Journal of General Virology

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“…Serum samples collected on days 0, 7 and 14 were tested for the presence of virus-neutralizing antibodies in a virus neutralization assay (Langedijk et al, 1996).…”

Section: Methodsmentioning

confidence: 99%

Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence

Antonis

Jong

Poel

et al. 2010

Journal of General Virology

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“…A blocking G peptide ELISA, as described by Langedijk et al (17), was used to determine bRSV G-specific antibody titers and is based on blocking of the binding of a bRSV G-specific MAb (MAb 20) with the coated peptide by peptide-specific antibodies that may be present in the serum.…”

Section: Methodsmentioning

confidence: 99%

Vaccine-Induced Immunopathology during Bovine Respiratory Syncytial Virus Infection: Exploring the Parameters of Pathogenesis

Antonis¹,

Schrijver²,

Daus³

et al. 2003

J Virol

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The bovine and human respiratory syncytial viruses cause severe lower respiratory tract infections. Effective vaccines against the respiratory syncytial viruses have been lacking since vaccine failures in the 1960s and 1970s. In this report, we describe a bovine respiratory syncytial virus (bRSV) challenge model in which both classical bRSV respiratory infection and vaccine-enhanced immune pathology were reproduced. The classical, formalin-inactivated (FI) bRSV vaccine that has been associated with vaccine failure was efficient in inducing high antibody titers and reducing viral loads but also primed calves for a far more serious enhanced respiratory disease after a bRSV challenge, thereby mimicking the enhanced clinical situation in FI human RSV (hRSV)-immunized and hRSV-infected infants in the 1960s. We show that immunization with FI-bRSV mainly primes a Th2-like inflammatory response that is characterized by a significant eosinophilic influx in the bronchial alveolar lung fluid and lung tissues and high levels of immunoglobulin E serum antibodies. The current model may be useful in the evaluation of new bRSV candidate vaccines for potency and safety.

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confidence: 99%

“…Antibodies to BRSV were found in cattle, goats, and horses but were not detected in sheep. 14,20 Recently, serologic evidence of RSV infection in swine has been reported. 5 Respiratory syncytial virus is recognized as an important pathogen in bovine respiratory tract disease, but the contribution, if any, of ORSV to the respiratory disease complex in cattle is unknown.…”

mentioning

confidence: 99%

“…14,20 A test based on epitopes of the G-glycoprotein has the potential to discriminate among antibodies induced by different RSV subgroups. 14,20 The purpose of this communication is to describe the development of BRSV and ORSV peptide-based enzyme immunoassays. The sensitivity, specificity, and positive and negative predictive values of each assay were determined.…”

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confidence: 99%

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Validation of Synthetic Peptide Enzyme Immunoassays in Differentiating Two Subgroups of Ruminant Respiratory Syncytial Virus

2001

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Abstract. Subgroup-specific peptide-based enzyme immunoassays from each respective G-glycoprotein of the ovine and the bovine respiratory syncytial virus (RSV) were developed to detect RSV-specific IgG responses in cattle. Antigenic peptides from the respective G-glycoprotein were identified from the extracellular central hydrophobic region (amino acids 158-189) located between 2 mucin-rich regions. These antigenic peptides identified by epitope mapping from each G-glycoprotein were synthesized and used to develop the subgroupspecific enzyme immunoassays. The negative cutoff for each enzyme immunoassay was established as the mean optical density of indirect immunofluorescent antibody-negative bovine sera plus 3 SDs. The sensitivity (82.9%) and specificity (100%) of the bovine enzyme immunoassay and the specificity (95.8%) of the ovine enzyme immunoassay were determined by comparison with indirect immunofluorescence (used as the ''gold standard''). The negative and positive predictive values were calculated for each assay. The presence of serum antibody to ovine RSV in cattle implies that this virus infects cattle and may contribute to the pathogenesis of bovine respiratory disease.Economic losses due to respiratory disease in the US cattle industry approximate $1 billion annually. 10 Respiratory syncytial virus (RSV) has been isolated from people (human RSV [HRSV]) and from several animal species, including cattle (bovine RSV [BRSV]), sheep (ovine RSV [ORSV]), and goats (caprine RSV). These viruses are members of the genus Pneumovirus in the family Paramyxoviridae. They are enveloped, single-stranded, nonsegmented, negativesense RNA viruses. 11 The genome encodes 10 viral proteins: 1B, 1C, N, P, M, SH, M2, L, the major glycoprotein (G), and the fusion (F) protein. 3,8 The G and F proteins are present in the membrane of infected cells and in the virus envelope. 16 The attachment protein G has been the most variable antigen among viral strains. 2 The proposed 3-dimensional model for the Gglycoprotein of BRSV contains a hydrophobic globular region (residues 158-189) bordered by 2 hydrophilic densely carbohydrate-coated mucin-like regions. 15 The G-glycoprotein gene from an ORSV strain has been cloned and sequenced and had greater homology to the BRSV gene (and its encoded protein) than to the HRSV gene (and its encoded protein). 2,4 However, the degree of base sequence differences between BRSV and ORSV suggests that 2 subgroups of an ungulate RSV exist. 2,15 Recent RNase protection assays, Received for publication October 26, 1999. reverse transcription polymerase chain reaction amplification studies, and a phosphoprotein profile analysis support the existence of 2 ruminant RSV subgroups. 1,9,17,18 Current knowledge concerning interspecies transmission of RSV strains is limited. Antibodies to BRSV were found in cattle, goats, and horses but were not detected in sheep. 14,20 Recently, serologic evidence of RSV infection in swine has been reported. 5 Respiratory syncytial virus is recognized as an important pathog...

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Type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein G

Cited by 38 publications

References 18 publications

Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence

Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence

Vaccine-Induced Immunopathology during Bovine Respiratory Syncytial Virus Infection: Exploring the Parameters of Pathogenesis

Validation of Synthetic Peptide Enzyme Immunoassays in Differentiating Two Subgroups of Ruminant Respiratory Syncytial Virus

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