Detection of wild animals as carriers of Leptospira by PCR in the Pantanal biome, Brazil

Vieira, Anahi S.; Narduche, Lorena; Martins, Gabriel; Péres, Igor Alexandre Hany Fuzeta Schabib; Zimmermann, Namor Pinheiro; Juliano, R. S.; Pellegrin, A. O.; Lilenbaum, Walter

doi:10.1016/j.actatropica.2016.08.001

Cited by 22 publications

(22 citation statements)

References 13 publications

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“…Herein, we found an important rate of PCR‐positive animals, around 30%. It is similar to that reported for other Brazilian biomes, as Pampa (Mayer et al., ) or Pantanal (Vieira et al., , ). Similarly, rodents and marsupials from the Peruvian Amazonian forest presented 29% of PCR positivity (Bunnell et al., ).…”

Section: Discussionsupporting

confidence: 91%

“…Since exposure to leptospiral antigens was searched, animals were considered seroreactive when titre was ≥50, as described by Vieira et al. () for detecting exposure on wildlife.…”

Section: Methodsmentioning

confidence: 99%

“…(OIE, 2014). Since exposure to leptospiral antigens was searched, animals were considered seroreactive when titre was ≥50, as described by Vieira et al (2016) for detecting exposure on wildlife. The sequences were edited using BioEdit v7.2.6.1 (Hall, 1999) and Molecular Evolutionary Genetics Analysis (MEGA) v.7 (Tamura, Stecher, Peterson, Filipski, & Kumar, 2013) (Tamura et al, 2013).…”

Section: Samplesmentioning

confidence: 99%

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PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Vieira

D’Andrea

Vilela

et al. 2019

Transbound Emerg Dis

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Leptospirosis is a common worldwide bacterial zoonosis and has been studied in One Health approaches. Small mammals are described as the most important maintenance reservoirs of several pathogens in nature, including leptospires. The aim of this study was to identify infection by leptospires among small mammals on the Atlantic forest biome and evaluate their potential as carriers of these spirochetes. A total of 153 small mammals belonging to orders Rodentia and Didelphimorphia (distributed on 17 genera and 22 species) were captured. Blood and kidney samples were collected from animals and a conventional PCR targeted on lipL32 gene was conducted on renal tissues. Species identification was performed in eight samples by sequencing of rrs gene. A total of 28% of the animals presented lipL32 PCR‐positive, and four pathogenic Leptospira species (L. interrogans, L. borgpetersenii, L. santarosai and L. noguchii) were identified. This study highlights the role of small mammals as carriers of leptospires on the Atlantic Forest representing a potential source of pathogenic Leptospira spp infection for both humans and domestic animals.

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Section: Discussionsupporting

confidence: 91%

“…Since exposure to leptospiral antigens was searched, animals were considered seroreactive when titre was ≥50, as described by Vieira et al. () for detecting exposure on wildlife.…”

Section: Methodsmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

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PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Vieira

D’Andrea

Vilela

et al. 2019

Transbound Emerg Dis

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“…The variety of serovars involved in heifer infection were similar to those reported in other studies conducted in the Pantanal region, where domestic-wild animal interface is a determining factor in the ecology of bovine leptospirosis (PELLEGRIN et al, 1999;JULIANO et al, 2011, MIASHIRO, 2013VIEIRA et al, 2016), and represent the largest reservoir populations of this farm, which maintain the agents in the environment. BHARTI et al (2003) reported serovar reservoirs of Hardjo and Wolffi in cows, Tarassovi in pigs, Bratislava in pigs and horses, Icterohaemorragiae in rodents, Hebdomadis in deer, and Pomona in cows and pigs.…”

Section: --------------------------------------------------------Prevsupporting

confidence: 83%

Reproductive performance of Nellore heifers raised in extensive system undergoing different vaccination protocols in fixed-time artificial insemination (FTAI)

Neto

Nogueira

Jardim³

et al. 2019

Cienc. Rural

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This study evaluated the reproductive performance of Nellore heifers without previous vaccination undergoing fixed-time artificial insemination (FTAI) and subsequent natural breeding under different vaccination protocols against infectious bovine herpesvirus 1 (BHV-1), bovine viral diarrhea virus (BVDV), and bovine leptospirosis (BL). A total of 763 animals were included; the group assignment was as follows: T1, control without vaccination; T2, vaccination against BL; T3, vaccination against BHV-1and BVDV; T4,vaccination against combination of BL + BHV-1and BVDV. Vaccination was started 30 days before FTAI (D-40), and a booster dose was administered at D-10. Serological tests were used to verify the occurrence rate of natural infection by BL, BHV-1, and BVDV. Initial pregnancy was diagnosed at D30, D60, and D120 after FTAI. Final pregnancy rates were evaluated by group, weight, body-condition score, occurrence of estrus, inseminator, bull, and occurrence of abortions or embryonic death. In all groups, results indicated seropositivity of 100% for leptospirosis, 77.0-94.0% for BHV-1, and 64.0-87.0% for BVDV. The vaccination protocol was not a significant influencing factor of pregnancy rate and embryonic or fetal mortality; whereas, animal weight at the beginning of the breeding season and presence of estrus at FTAI were significant influencing factors of pregnancy rate. The vaccination protocol had no interference with the reproductive performance of the experimental groups.

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“…Contudo, a fácil adaptabilidade em ambientes variados promove efeitos negativos para a espécie, principalmente quando o hábitat é compartilhado com seres humanos e animais domésticos, de companhia e de produção. Como resultado desse compartilhamento, diversos parasitas comuns a outras espécies têm sido relatados em lobinhos, como bactérias (8)(9)(10) , vírus (11)(12)(13) e protozoários gastrintestinais e sanguíneos (14)(15)(16)(17)(18) .…”

Section: Introductionunclassified

VALORES HEMATOLÓGICOS DE LOBINHOS (Cerdocyon thous) DO PANTANAL, MATO GROSSO DO SUL, BRASIL NATURALMENTE INFECTADOS E NÃO INFECTADOS POR Trypanosoma cruzi e T. evansi

et al. 2019

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Resumo O objetivo do trabalho foi avaliar a influência do sexo, da sazonalidade e de infecções por Trypanosoma cruzi e Trypanosoma evansi nos valores hematológicos de lobinhos (Cerdocyon thous) que habitam o Pantanal Sul-Mato-Grossense. Entre novembro de 2015 e outubro de 2016, foram amostrados 48 lobinhos. Os valores hematológicos mensurados foram as contagens de eritrócitos e leucócitos, volume globular e volume corpuscular médio. Consideramos como parasitados os animais positivos para T.cruzi e T. evansi em qualquer um dos testes diagnósticos utilizados. Observamos que sete (14.5%) lobinhos encontraram-se parasitados somente por T.cruzi, sete por T. evansi, e nove (19%) estavam coinfectados. Os animais parasitados por T.cruzi mostraram um aumento significativo das contagens de leucócitos (14.7 x 103) em relação aos animais não parasitados (10.4 x 103), parasitados por T. evansi (12.4 x 103) e coinfectados (12.9 x 103). Observamos diferença significativa em relação aos valores médios de eritrócitos e volume corpuscular médio entre os períodos de cheia e seca: 2.6 x 106 e 165, e 3.4 x 106 e 132, respectivamente. Todos apresentaram macrocitose não confirmada morfologicamente.

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Detection of wild animals as carriers of Leptospira by PCR in the Pantanal biome, Brazil

Cited by 22 publications

References 13 publications

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Reproductive performance of Nellore heifers raised in extensive system undergoing different vaccination protocols in fixed-time artificial insemination (FTAI)

VALORES HEMATOLÓGICOS DE LOBINHOS (Cerdocyon thous) DO PANTANAL, MATO GROSSO DO SUL, BRASIL NATURALMENTE INFECTADOS E NÃO INFECTADOS POR Trypanosoma cruzi e T. evansi

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