Detection of VEB-1, OXA-10 and PER-1 genotypes in extended-spectrum β-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients

Mirsalehian, Akbar; Feizabadi, Mehdi; Nakhjavani, F Akbari; Jabalameli, Fereshteh; Goli, Hamid Reza; Kalantari, Narges

doi:10.1016/j.burns.2009.01.015

Cited by 94 publications

(82 citation statements)

References 20 publications

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“…The treatment of infections caused by these resistant organisms is increasingly complicated owing to the high levels of resistance to the most commonly prescribed antibiotics in hospital settings 27 . Several studies carried out by Mirsalehian et al 28 , Farshadzadeh et al 29 , and Shakibaie et al 30 covering three ESBL: extended spectrum β-lactamase.…”

Section: Discussionmentioning

confidence: 99%

“…In a study from Iran, Shahcheraghi et al reported that 24%, 17%, and 0% of MDRPA isolates harbored the bla VEB , bla PER , and bla GES genes, respectively 23 . Mirsalehian et al reported that 74.6%, 49.2%, and 31.3% of ESBL-producing isolates of P. aeruginosa collected from patients at Motahhari Burn Hospital in Tehran were positive for bla OXA-10 , bla PER-1 , and bla VEB-1 , respectively 28 . In another study conducted by Rafiee et al at the same burn center, it was shown that 21.6% of P. aeruginosa isolates carried the bla PER-1 gene…”

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Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

Amirkamali

Naserpour-Farivar

Azarhoosh

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:Pseudomonas aeruginosa is one of the most common nosocomial pathogens. The emergence of extended spectrum β-lactamases (ESBLs) has been increasingly reported as a major clinical concern worldwide. The main aim of the present study was to determine the distribution of bla OXA , bla PER-1, bla VEB-1, and bla GES-1 genes among ESBL-producing P. aeruginosa isolated from two distinct provinces in Iran. Methods: In this study, a total of 75 (27.5%) ESBL-producing isolates were identified from 273 P. aeruginosa isolates collected from patients in Qazvin and Tehran. Phenotypic detection of ESBLs and antimicrobial susceptibility testing were performed according to the Clinical and Laboratory Standards Institute guidelines. PCR and sequencing were employed to detect bla OXA-1 , bla OXA , bla GES-1, bla PER-1 , and bla VEB-1 genes. Isolate genetic relationships were evaluated by repetitive extragenic palindromic sequence-based PCR (REP-PCR). Results: In total, 59 (78.7%) of the ESBLproducing isolates showed multidrug resistance. The highest rates of susceptibility were observed against colistin (75 isolates, 100%) and polymyxin B (75, 100%) followed by amikacin (44, 58.7%), and piperacillin-tazobactam (40, 53.3%). The bla OXA-1 (37.3%) gene was the most common of the genes investigated, followed by bla OXA-4 (32%), bla GES-1 (16%), and bla VEB-1 (13.3%). REP-PCR identified three different genotypes: types A (89.3%), B (6.7%), and C (4%). Conclusions: We found a significant presence of bla OXA-1 , bla OXA-4 , bla GES-1, and bla VEB-1 genes among P. aeruginosa isolates, highlighting the need for suitable infection control strategies to effectively treat patients and prevent the further distribution of these resistant organisms.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

Amirkamali

Naserpour-Farivar

Azarhoosh

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…OXA-10-like enzymes are also frequent, as is the case in several countries (Mirsalehian et al, 2010;Strateva & Yordanov, 2009). VIM appears to be the most common MBL, as is also the case in many countries worldwide (Lagatolla et al, 2004;Valenza et al, 2010;Lee et al, 2003;Strateva & Yordanov, 2009).…”

Section: B-lactamases In Gram-negative Bacteria In Saudi Arabiamentioning

confidence: 90%

The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia

Yezli

Shibl

Memish

2015

Journal of Medical Microbiology

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Resistance to b-lactams among Gram-negative bacteria is a worldwide issue. Increased prevalence of extended-spectrum b-lactamase (ESBL)-producers and the dissemination of carbapenem-resistance genes are particularly concerning. ESBL-producing strains are common in the Kingdom of Saudi Arabia, particularly among the Enterobacteriaceae, and carbapenem resistance is on the increase, especially among the non-fermenters. b-lactamase production is a major mechanism of resistance to these agents and although b-lactamase-producing strains have been documented in the Kingdom, relatively few reports characterized the molecular basis of this production. Nevertheless, available data suggest that CTX-M (CTX-M-15 in particular) is the predominant ESBL in the Enterobacteriaceae, with SHV also being prevalent in Klebsiella pneumoniae. Carbapenem resistance in the latter is mainly due to OXA-48 and NDM-1. In Pseudomonas aeruginosa, VEB-like enzymes are the most common ESBLs, and VIM is the prevalent metallo-b-lactamase. OXA-10 extended-spectrum enzymes are also frequent. PER and GES ESBLs have been reported in Acinetobacter baumannii, and oxacillinases (OXA-23 in particular) are the dominant carbapanamases in this species. IntroductionInfections due to Gram-negative bacteria are a leading cause of morbidity and mortality worldwide (Giske et al., 2008). Antimicrobial agents are a major therapeutic tool in the treatment of such infections. However, antimicrobial resistance among Gram-negative bacteria, particularly among the Enterobacteriaceae and the non-fermenters, has become one of the most serious public health concerns worldwide (Giske et al., 2008;Livermore, 2012). The global dissemination of resistance has recently received much attention, especially following reports of the international spread of multi-resistant Enterobacteriaceae, particularly strains resistant to cephalosporins due to the production of CTX-M-type extended-spectrum b-lactamases (ESBLs) and strains producing carbapenemases such as KPC and NDM (Nordmann et al., 2011;Johnson & Woodford, 2013). The spread of resistance determinants is facilitated by a number of factors, including presence on genetic mobile elements, antibiotic misuse, poor infection control practices, and increased international travel. In this context Saudi Arabia is particularly relevant as it is annually a host for more than 4 million Muslim pilgrims from over 180 countries worldwide in the Hajj and Umra seasons. In addition, up to 6 million of the Kingdom's population are expatriates, mainly from south and east Asia, where antimicrobial resistance is rife, including to carbapenems (Yong et al., 2009). These factors could potentially make Saudi Arabia a hot spot for the collection of multidrug-resistant strains and the spread of antibiotic resistance around the world.We previously reported on the antimicrobial resistance among the key Gram-negative bacteria in Saudi Arabia and found increased prevalence of b-lactam-resistant strains, including ESBL-producers, and carbapenem resistance (Y...

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“…Amongst the results from our country, 24% of ESBL positive isolates in the study of Shacheraghi et al contained this gene (20). Furthermore, in Tehran, Mirsalehian demonstrated that 49.25% and 31.34% of ESBL positive isolates collected from burn patients amplified bla PER-1 and bla VEB-1 genes, respectively (21). However, in Korea, none of the P. aeruginosa isolates could amplify the bla VEB-1 gene (10).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

Davodian

Sadeghifard

Ghasemian

et al. 2015

Arch Pediatr Infect Dis

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Background: Pseudomonas aeruginosa is considered as a leading cause of nosocomial infections. Burn and wound infections are mainly caused by multidrug-resistant P. aeruginosa isolates. Drug resistance frequently occurs among nosocomial isolates and can usually resist a myriad of antibiotics such as novel β-lactam antibiotics. Detection of multidrug-resistant isolates could assist better drug administration. Objectives: The aim of this study was to detect Extended Spectrum Beta-Lactamases (ESBL) positive wound isolates and the genes encoding bla VEB-1 ESBL among wound isolates of P. aeruginosa. Materials and Methods: A total of 89 wound isolates of P. aeruginosa were collected from patients (47% (n = 42) were male and 53% (n = 47) were female) at six Iranian hospitals between years 2009 and 2011. Antibiotic susceptibility and phenotypic ESBL production tests were conducted. The combined disk was used to determine ESBLs production. The bla VEB-1 gene was detected with the polymerase chain reaction (PCR). Results: The majority of the wound isolates were resistant to augmentin (90%, n = 80) and cefpodoxime (87.6%, n = 78). However, the majority was susceptible to imipenem and meropenem. Fifty-eight (42%) wound isolates were ESBL positive. The antibiotic resistance amongst ESBL positive isolates was relatively higher than ESBL negative isolates. Twenty-three (40%) ESBL-positive isolates amplified the bla VEB-1 gene. Conclusions: More than behalf of the wound isolates were ESBL positive, and the presence of bla VEB-1 was determined in less than half of these isolates. Fortunately, resistance to imipenem and meropenem was low.

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Detection of VEB-1, OXA-10 and PER-1 genotypes in extended-spectrum β-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients

Cited by 94 publications

References 20 publications

Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia

Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

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