Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

Davodian, Elham; Sadeghifard, Nourkhoda; Ghasemian, Abdolmajid; Noorbakhsh, Samileh

doi:10.5812/pedinfect.26362

Cited by 9 publications

(6 citation statements)

References 22 publications

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“…About 0.3% of the total genes in P. aeruginosa are responsible for antibiotic resistance (14). Approximately 77% of ICU isolates were ESBL producers which is comparable with previous studies (15,16). Susceptibility to clavulanic acid is a diagnostic method.…”

Section: Antibiotic Susceptibility Testsupporting

confidence: 70%

“…In the study by Shacheraghi et al in ESBL positive isolates, the prevalence of bla VEB-1 and bla PER-1 was 100% and 68.3%, respectively (18), but a low number (5.8%) of imipenem-resistant isolates contained bla PER-1 in another study. In a study by Davodian et al, among wound isolates the 90% and 88% were resistant to augmentin and cefpodoxime, 40% of them being bla VEB1 positive (15). Besides this, in our study, resistance to imipenem and meropenem was low (18.4 and 20%, respectively), suggesting that the low presence of carbapenemhydrolyzing enzymes in addition to class A enzymes.…”

Section: Discussionsupporting

confidence: 49%

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Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Ghasemian¹,

Mostafavi²,

Eslami³

et al. 2018

Immunopathol Persa

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Introduction:Pseudomonas aeruginosa isolates are among the most common pathogens causing nosocomial infections. They are intrinsically resistant to most of antibiotics such as novel β-lactams and therefore, can develop resistance during treatment, culminating in failure in remedy. Objectives: The aim of this study was to detect the genes encoding class A extended-spectrum betalactamases (ESBLs) such as PER-1, VEB-1 and PSE-1 among P. aeruginosa isolates from intensive care unit (ICU) patients. Materials and Methods: A total of 65 isolates were collected from ICU in three hospitals of Tehran in 2016. The antibiotic susceptibility test was conducted according to Clinical and Laboratory Standards Institute (CLSI) guideline. MIC of ceftazidime was done with agar dilution method. The combine disk test was performed for detection of isolates producing ESBLs. Polymerase chain reaction (PCR) was performed to detect the PER-1, VEB-1 and PSE-1 genes using specific primers. Results: Fifty-four percent (n=38) of patients were male and 46% (n=27) were female. The majority of ICU isolates were resistant to augmentin (93.8%, n=61) and cefpodoxime (84.8%, n=56). Fifty (77%) isolates were ESBL positive, among which 94% (n=47) harbored PER-1 gene followed by 52% (n=26) VEB-1 and 16% (n=8) PSE-1 genes. Conclusion: Concomitance presence of bla PER1 and bla VEB1 was observed among 10 isolates, and 7 amplified all these three genes. A high number of ICU P. aeruginosa isolates were ESBL producers. The frequency of bla VEB1 and bla PER1 were relatively high, while bla PSE1 was detected among a low number of isolates. Moreover, resistance to carbapenems was low. It is necessary to follow up ICU centers because of drugresistant P. aeruginosa isolates. AbstractCitation: Ghasemian A, Shokouhi Mostafavi SK, Eslami M, Vafaei M, Nojoomi F, Hasanvand F. Antibiotic resistance and presence of bla PER-1 , bla VEB-1 and bla PSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings. Immunopathol Persa. 2018;4(2):e26.

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Section: Antibiotic Susceptibility Testsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 49%

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Ghasemian¹,

Mostafavi²,

Eslami³

et al. 2018

Immunopathol Persa

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“…With respect to bacterial resistance against the third generation cephalosporins, infected chronic wound isolates exhibited the highest rates of resistance ranging from 70% to 100% ( Fig 1 ) against cefixime (gramocef-0-400 ® ) and cefpodoxime (Ximeprox ® ). Findings from previous similar studies revealed comparable resistance of infected chronic wound isolates ranging from 87.6% to 100% resistance against cefpodoxime (Ximeprox ® ) and cefixime (gramocef-0-400 ® ) [ 17 , 18 ]. Therefore the therapeutic benefit of cefixime (gramocef-0-400 ® ) and cefopoxime 200mg (Ximeprox ® ) is extremely low to manage infected chronic wounds and continued use of these antibiotics will burden the patients with long hospital stays, high treatment costs, further delay of wound healing and development of severe invasive bacterial infections [ 19 ].…”

Section: Discussionmentioning

confidence: 96%

Resistance pattern of infected chronic wound isolates and factors associated with bacterial resistance to third generation cephalosporins at Mbarara Regional Referral Hospital, Uganda

Tungotyo

Twinomujuni

2021

PLoS ONE

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Background The objectives of this study were; (I) to determine the proportion of pathogens isolated from patients with infected chronic wounds in the surgical ward of MRRH that are resistant to the third-generation cephalosporins and (II) to determine the factors associated with resistance to third-generation cephalosporins in the surgical ward of MRRH. Method(s) This study was a descriptive analytical survey of bacterial isolates from infected chronic wounds among patients admitted in the surgical ward of MRRH, Uganda. Seventy five (75) study participants were recruited in the study using convenient sampling technique. Bacterial culture and identification was performed using standard microbiology laboratory procedures whereas broth microdilution method was used to establish the susceptibility of the identified pathogens. Data for objective one (1) was summarized as proportions while the categorized variables were analyzed using logistic regression to determine whether they were associated with the resistance patterns. The level of significance was preset at 5% and p-values less than 0.05 were considered statistically significant. Results Generally, all isolates had complete susceptibility (100%) to Cefoperazone+Sulbactam 2g except 7.1% of proteus spp that were resistant. Of all the bacterial isolates studied, Staphylococcus aureus, Enterobacter agglomerans, providencia spp and pseudomonas earuginosa had complete resistance (100%) to Cefopodoxime 200mg while providencia spp and pseudomomas earuginosa had complete resistance (100%) to Cefixime 400mg and cefotaxime 1g. Finally, higher odds of bacterial resistance to more 2 brands of the third generation cephalosporins were observed among participants who had prior exposure to the third generation cephalosporins (OR, 2.22, 95% CI, 0.80–6.14), comorbidities (OR, 1.76, 95% CI, 0.62–4.96) and those who had more than two hospitalizations in a year (OR, 1.39, 95% CI 0.46–4.25). However, multivariate logistic regression was not performed since no factor was significantly associated with resistance to more than two brands of third generation cephalosporins (p >0.05). Conclusion This study found that cefixime and cefpodoixme had high rates of resistance and should not be used in routine management of infected chronic wounds. In addition, the factors investigated in this study were not significantly associated with bacterial resistance to more than two brands of third generation cephalosporins.

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“…Difficulties in eradication of drug-resistant infections have been a critical issue in recent years. [2][3][4][5][6] Great therapeutic properties of aquatic sources including sterols, peptides, terpenes and nitrogen compounds, macrolides, acid-fat derivatives, prostaglandins, and other alkaloids have been revealed. 7 Bacterial infections cause many deaths annually.…”

Section: Enteric Pathogensmentioning

confidence: 99%

Antimicrobial Features of Cerastoderma and Didacna Double Basins Peptides

Ghasemian¹,

Nojoomi²,

Najafiolya³

et al. 2018

Int J Enteric Pathog

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Background: In recent years, high attention has been given to the biological activities of natural compounds and their potential antimicrobial properties. Objective: In this study, the antibacterial properties of the extracts from tissue and peptides of Cerastoderma and Didacna were studied. Materials and Methods: samples of Cerastoderma and Didacna were collected and washed. Then, the soft tissues were cut and powdered, and concentrations of 16, 8, 4, 2, 1 and 0.5 of chloroform, ethanol and methanol, and in addition extract of enzymatic hydrolysis were prepared, and their antibacterial activities against Staphylococcus aureus, Escherichia coli and Salmonella paratyphi were investigated. The disc diffusion method was used for the evaluation of strains susceptibility. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated for bacterial growth inhibition. Results: Methanolic and ethanolic extracts from Cerastoderma demonstrated higher growth inhibitory effects compared to those from Didacna on E. coli and S. paratyphi and exhibited similar activities against S. aureus at concentrations 16 and 8 ug/mL. In addition, chloroform extracts of Cerastoderma and Didacna displayed similar inhibitory effects on S. paratyphi and S. aureus at concentrations 16 and 8 ug/mL which was a suitable effect, and the extract from Cerastoderma was more effective. MIC and MBC of methanolic extracts were at the lowest level, especially against S. aureus. Conclusion: It was revealed that Cerastoderma and Didacna extracts were effective as antibacterial compounds on S. aureus, E. coli and S. paratyphi species as natural agents.

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Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

Cited by 9 publications

References 22 publications

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Resistance pattern of infected chronic wound isolates and factors associated with bacterial resistance to third generation cephalosporins at Mbarara Regional Referral Hospital, Uganda

Antimicrobial Features of Cerastoderma and Didacna Double Basins Peptides

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