Detection of the serotonin-modulated protein fraction and its role in organization of passive avoidance behavior in rats

Gasanov, G. G.; Мехтиев, А. А.

doi:10.1007/bf00841123

Cited by 3 publications

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“…Dual-Way of Suppressive Action of Antibodies to Serotonin-Modulating Anticonsolidation Protein on Drug Addiction in Rats Research AA Mekhtiev conditioned passive avoidance and shuttle box models, as well as in electrophysiological studies on identified command neurons of the grapes snails [8][9][10][11]. In addition to the fact that in the above mentioned models SMAP induces the same effects as serotonin itself, it, as the results of studies of excitability and chemosensitivity of the identified command neurons of the snails have shown, as well possesses with several-time stronger serotonergic activity.…”

Section: Researchmentioning

confidence: 99%

“…In our earlier studies in the rat brain cortex was identified and from the whole rat brain was purified serotonin-modulating anticonsolidation protein (SMAP) [8]. As showed biochemical and electrophysiological studies, SMAP is in linear relation with serotonin and realizes serotonin functions on sub-cellular level in the nervous cells [8][9][10]. Intracerebral administration of this protein to the rats prior to learning sessions leads to significant impairment of memory consolidation in the conditioned models of passive avoidance and shuttle box [8,11].…”

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confidence: 98%

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Dual Way of Suppressive Action of Antibodies to Serotonin Modulating Anticonsolidation Protein on Drug Addiction in Rats

Aa¹,

Shm²

2019

Neuropsychiatry

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Analysis of engagement of serotonin-modulating anticonsolidation protein (SMAP) in formation of drug addiction to morphine in self-administration model in the rats via application of the anti-SMAP antibodies MethodsIndirect ELISA-test, morphine self-administration model, conditioned model of alternative running, conditioned model of instrumental differentiation. ResultsUpregulation of SMAP by 67% in the brain cingulate cortex of the rats with stable level of morphine intake, while no changes of SMAP level were noticed in hypothalamus. Intramuscular administration of the anti-SMAP antibodies to the rats with stable level of morphine intake leads to significant suppression of morphine consumption for 8 days. Administration of non-immune γ-globulins does not have any effect on morphine consumption. Intra-cerebral administration of the anti-SMAP antibodies leads to significant acceleration and strengthening of memory formation in complicated conditioned alternative running and instrumental differentiation models. ConclusionApplication of the anti-SMAP antibodies induces suppression of elaborated drug addiction in the rats, first, through blockade of intra-cellular transduction of serotonin signal in the concerned nervous cells and, second, due to formation of negative memory on inefficacy of lever pressing to get bright positive emotions after morphine self-administration.

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Section: Researchmentioning

confidence: 99%

mentioning

confidence: 98%

Dual Way of Suppressive Action of Antibodies to Serotonin Modulating Anticonsolidation Protein on Drug Addiction in Rats

Aa¹,

Shm²

2019

Neuropsychiatry

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“…Серотонин-модулируемый антиконсолидационный белок, или СМАБ, первоначально выявлен в затылочной области коры головного мозга крысы после аппликации серотонина (Mekhtiev, 2000). Название белка обусловлено, с одной стороны, установленной зависимостью его содержания в нервных клетках головного мозга млекопитающих от уровня серотонина (Gasanov & Mekhtiev, 1991;Mekhtiev et al, 2003), а с другойнегативным влиянием СМАБ на формирование долговременной памяти у животных (Mekhtiev, 2000). Нарушение консолидации следов памяти под действием СМАБ наблюдалось как у высших позвоночных (млекопитающих) в различных условно-рефлекторных моделях обучения (Guseinov & Mekhtiev, 2012;Mekhtiev et al, 2015), так и у низших (костистых рыб) при введении им СМАБ в желудочек мозга перед обучением (Garina & Mekhtiev, 2014).…”

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The role of neuro-specific dihydropyrimidinase-related protein 2 (dpyl2) in spatial memory formation in teleosts

Гарина

Bolshakov

Toropygin

et al. 2018

Regul. Mech. Biosyst.

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This article presents the results of an experiment on the influence of serotonin-modulating anticonsolidation protein (SMAP) on the spatial memory formation of juvenile goldfish Carassius auratus (L.) in a maze with food reinforcement. Three experimental fish groups were formed: (1) intact animals, (2) experimental group (fish injected ICV with SMAP in 24 h before the beginning of training; 2 μl, 1.5 mg·ml–1), (3) active control group (fish injected ICV with inactivated SMAP). Goldfishes of the experimental group demonstrated the lowest capability for spatial recognition: the maximum level of performance of the task was on 4th day of the training – 38%, while the values of this index in fishes of the control and intact groups were 70% and 63% respectively. In general, throughout the period of the training the average value of task performance was 16% in the SMAP-injected fish (in the control and intact groups – 42% and 53%, respectively). By using Ds-Na-polyacrylamide gel electrophoresis SMAP compositeon has been revealed. It was found that it consists of 10–12 protein components, among which four proteins dominated. They were identified by mass spectrometry MALDI-TOF: spectrin, dihydropyrimidinase-related protein 2 (DPYL2), tubulin and actin. It has been suggested that the most likely candidate responsible for the negative effects of SMAP on fish memory formation is DPYL2. It was hypothesized that anticonsolidation effect of SMAP is caused by the effect of DPYL2 which blocks the growth of axons or its cytostatic activity which leads to disorders in formation of new neurons in the brain as a result of learning.

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“…Therefore, investigation of serotonin effects on genome activity can clarify the mechanisms of its participation in memory consolidation. Previously, we identified and purified a new serotonin-modulated brain protein SMP-69 [1,5].This study analyzed the effects of antibodies (AB) to SMP-69 on memory processes and genomic activity in rat neurons. …”

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Genetic mechanisms of the involvement of SMP-69 protein in memory consolidation

Мехтиев

1999

Bull Exp Biol Med

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Intracerebral administration of antibodies to SMP-69 protein to rats 24 h prior to passive avoidance conditioning impaired memory processes, while the administration 48 h after learning had no such effect. Activation of RNA and protein synthesis was observed 24 h after the administration. It is suggested that the impaired consolidation of memory traces is due to the synthesis of "anticonsolidation" proteins. Key Words: antibodies to SMP-69 protein; memoly consolidation; transcription; translationStudies on different models of learning have shown that serotonin is involved in the consolidation of memory traces [4,8,12,14]. Both the decrease in the brain content of serotonin after destruction of serotoninergic neurons with intracerebrally administered specific neurotoxins [2] and experimental elevation of brain serotonin [ 15] impair memory formation. The involvement of serotonin in memory consolidation is determined by its regulatory effect on genetic apparatus of neurons [9]. Therefore, investigation of serotonin effects on genome activity can clarify the mechanisms of its participation in memory consolidation. Previously, we identified and purified a new serotonin-modulated brain protein SMP-69 [1,5].This study analyzed the effects of antibodies (AB) to SMP-69 on memory processes and genomic activity in rat neurons. MATERIALS AND METHODSPolyclonal AB to SMP-69 were isolated by affinity chromatography from the serum of rabbits immunized for 3-4 months with SMP-69 [7]. A. I. Karaev Institute of Physiology, Azerbaijan Academy of Sciences, BakuPassive avoidance conditioning was used to assess the effect of AB on memory processes. Experimental chamber consisted of 2 compartments (illuminated and dark) connected by a narrow passage. During the acquisition procedure, the rat was placed into the light compartment and after transition into the dark compartment received intermittent footshock (0.8 mA) applied through electrified floor. After shock the animal returned to the light compartment and was taken out. In the retention test (10-rain session) the animal was placed into the light compartment and was not shocked when entering the dark compartment. During this test the following indices were measured: the latency of entering the dark compartment, the time spent in each compartment and near the entrance to the dark compartment.Preparations were administered in the left lateral ventricle under ether anesthesia. The rats were divided into experimental and control groups (n=12 each). The experimental rats received 10/al AB (1.5 mg/ml), control rats received the same volume of nonimmune y-globulins. In series I, the preparations were administered 24 h prior to learning passive avoidance task; the retention of the learned response was tested 48 h afterwards. In series II, animals received the preparations 48 h after training and were tested for retention 24 h postinjection.0007 -4888/99/0008-0779522.00 ~ Kluwer Academic/Plenum Publishers

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Detection of the serotonin-modulated protein fraction and its role in organization of passive avoidance behavior in rats

Cited by 3 publications

References 5 publications

Dual Way of Suppressive Action of Antibodies to Serotonin Modulating Anticonsolidation Protein on Drug Addiction in Rats

Dual Way of Suppressive Action of Antibodies to Serotonin Modulating Anticonsolidation Protein on Drug Addiction in Rats

The role of neuro-specific dihydropyrimidinase-related protein 2 (dpyl2) in spatial memory formation in teleosts

Genetic mechanisms of the involvement of SMP-69 protein in memory consolidation

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