Detection of telomerase activity in saliva from oral squamous cell carcinoma patients

Zhong, Lai‐ping; Chen, G.-F.; Xu, Zefeng; Zhang, Xiang; Ping, Fei-yun; Zhao, Song

doi:10.1016/j.ijom.2004.10.007

Cited by 42 publications

(20 citation statements)

References 25 publications

(30 reference statements)

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“…Many attempts have been made in the past few years to use saliva to discover biomarkers for diseases localized in the oral cavity or in the head/neck region. Some diseases for which saliva has been used as a medium for biomarker discovery include oral cancer squamous cell carcinoma [57–65], head and neck squamous cell carcinoma [66–68], and Sjogren’s syndrome [69–77]. In addition, saliva has shown promise for use in the detection of many nonoral (i.e., nonproximal) systemic diseases.…”

Section: Discussionmentioning

confidence: 99%

Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

et al. 2008

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Introduction Saliva is a body fluid that holds promise for use as a diagnostic fluid for detecting diseases. Salivary proteins are known to be heavily glycosylated and are known to play functional roles in the oral cavity. We identified N-linked glycoproteins in human whole saliva, as well as the N-glycoproteins in parotid, submandibular, and sublingual glandular fluids. Materials and Methods We employed hydrazide chemistry to affinity enrich for N-linked glycoproteins and glycopeptides. PNGase F releases the N-peptides/proteins from the agarose-hydrazide resin, and liquid chromatography–tandem mass spectrometry was used to identify the salivary N-glycoproteins. Results A total of 156 formerly N-glycosylated peptides representing 77 unique N-glycoproteins were identified in salivary fluids. The total number of N-glycoproteins identified in the individual fluids was: 62, 34, 44, and 53 in whole saliva, parotid fluid, submandibular fluid, and sublingual fluid, respectively. The majority of the N-glycoproteins were annotated as extracellular proteins (40%), and several of the N-glycoproteins were annotated as membrane proteins (14%). A number of glycoproteins were differentially found in submandibular and sublingual glandular secretions. Conclusions Mapping the N-glycoproteome of parotid, submandibular, and sublingual saliva is important for a thorough understanding of biological processes occurring in the oral cavity and to realize the role of saliva in the overall health of human individuals. Moreover, identifying glycoproteins in saliva may also be valuable for future disease biomarker studies.

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Section: Discussionmentioning

confidence: 99%

Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

et al. 2008

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“…The studies have examined genomic and proteomic targets such as DNA aberrations, mRNAs, enzymes, cytokines, growth factors, metalloproteinases, telomerase, cytokeratins etc. in oral cancer [13,17,[27][28][29][30][31]. The first report of saliva as a diagnostic tool for oral cancer detection was published in 2000 by Liao et al [32].…”

Section: Salivary Genomics and Proteomics Biomarkers In Oral Cancermentioning

confidence: 99%

“…Zhong et al [30] found 75% positive expression of telomerase in saliva of oral cancer patients suggesting its usefulness as a supportive marker to diagnose oral cancer and also suggested that human telomerase reverse transcriptase (hTERT) analysis may be a potential biomarker for the diagnosis of oral cancer. Telomerase is a ribonucleoprotein which aid to elongate repeat sequence at the end of the chromosomes.…”

Section: Salivary Genomics and Proteomics Biomarkers In Oral Cancermentioning

confidence: 99%

“…[27] IL-8, TNF-a Telomerase Increased telomerase activity found in saliva of oral cancer patients suggested that the telomerase in saliva could be useful biomarker for oral cancer. [30] p53 Autoantibodies Presence of p53 autoantibodies in saliva as well as serum of oral cancer patients demonstrated that its detection in saliva can offer a non-invasive method for the detection of a subset of tumors with p53 aberrations.…”

Section: Salivary Genomics and Proteomics Biomarkers In Oral Cancermentioning

confidence: 99%

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A Review on Salivary Genomics and Proteomics Biomarkers in Oral Cancer

et al. 2011

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Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce the morbidity and mortality associated with this disease. Sensitive and specific biomarkers for oral cancer are likely to be most effective for screening, diagnosis, staging and follow-up for this dreaded malignancy. Unlike other deep cancers, oral cancer is located in oral cavity. Hence, the direct contact between saliva and oral cancer lesion makes the measurement of tumor markers in saliva an attractive alternative to serum and tissue testing. The DNA, RNA and protein molecules derived from the living cancer cells can be conveniently obtained from saliva. Thus, salivary biomarkers, a non-invasive alternative to serum and tissue-based biomarkers may be an effective modality for early diagnosis, prognostication and monitoring post therapy status. In the current post-genomic era, various technologies provide opportunities for high-throughput approaches to genomics and proteomics; which have been used to evaluate altered expressions of gene and protein targets in saliva of oral cancer patients. The emerging field of salivary biomarkers has great potentials to prove its clinical significance to combat oral cancer. Hence, we have reviewed importance of several salivary genomics and proteomics biomarkers for oral cancer.

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“…Detection of telomerase activity in saliva of OSCC was performed by Zhong et al, (2005) and they detected telomerase positivity in 75% of cases suggesting that telomerase detection could be used as assistant marker in OSCC. Telomerase is a ribonucleoprotein aid to elongate repeat sequence at the end of the chromosomes.…”

Section: Genomic Markersmentioning

confidence: 99%

Salivary Diagnostics in Oral Cancer

Sridharan¹

2012

Oral Cancer

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Detection of telomerase activity in saliva from oral squamous cell carcinoma patients

Cited by 42 publications

References 25 publications

Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

A Review on Salivary Genomics and Proteomics Biomarkers in Oral Cancer

Salivary Diagnostics in Oral Cancer

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