Detection of specific antibodies of classes G and M to bovine leukemia virus in the blood serum

Gulyukin, Mikhail; Капустина, О. А.; Ezdakova, I. Yu.; Valtsiferova, S.V.; Stepanova, Tatyana; Anoyatbekov, M.

doi:10.36233/0507-4088-2019-64-4-173-177

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…Динаміка титрів антитіл у дослідних кролів, визначена в РІД Найвища концентрація антитіл у сироватці крові вивлялась на 90-у добу після введення вірусовмісного матеріалу; на 150-180 добу після зараження антитіла зникли з крові, що пов'язуємо з роллю імуноглобулінів класу G в процесах фіксації комплементу та опсонізуючих реакціях, які є обов'язковими під час нейтралізації антигенів, а не імуноглобулінів класу А як нейтралізаторів токсинів у перші дні введення інокуляту (Gulyukin et al, 2019).…”

Section: таблицяunclassified

“…Якісний склад лімфоцитів також підлягав суттєвим змінам, а саме: характеризувався появою бласних та рідеровських клітин та клітин з подвійними ядрами. Такі зміни в лейкограмі кролів дослідної групи характерні для серологічно позитивних корів на гематологічній стадії розвитку лейкозного процесу та корелюють із результатами вітчизняних та закордонних авторів (Alvarez et al, 2013;Gulyukin et al, 2019;Porta et al, 2019;Zyrianova & Kovalchuk, 2020).…”

Section: таблицяunclassified

“…Як відомо, існують гомологічні та гетерологічні види тварин і саме на таких видах зазвичай проводять експериментальні роботи з відтворення лейкозу ВРХ. Наразі економічно доцільним є пошук економічно зручної моделі для проведення експериментальної роботи щодо відтворення дії вірусу лейкозу ВРХ (Gulyukin et al, 2019, Krasnikova et al, 2019Porta et al, 2019).…”

Section: обговоренняunclassified

“…У кролів інфекційний процес, зумовлений вірусом лейкозу великої рогатої худоби, в більшості випадків характеризується появою специфічних антитіл і наявністю в крові геному BLV. В деяких випадках у тварин знижується Т-клітинна чутливість до стимуляції фітолектином і ознаки конюнктивіту, риніту, втрати живої маси (Gulyukin et al, 2019;Krasnikova et al, 2019). Експериментально підтверджено 100 % можливість зараження лейкозом великої рогатої худоби кролів -виду, філогенетично цілком віддаленого від природного господаря цього вірусу, причому не тільки при параентеральному введенні крові інфікованої тварини, а й з молоком -через шлунково-кишковий тракт (Sentsui et al, 1988;Shabeykin et al, 2019).…”

Section: обговоренняunclassified

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Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Романишина

Feschenko

Rinyak³

et al. 2020

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Bovine leukemia virus (BLV) is an infectious disease of cattle, causing high economic losses worldwide, especially in the field of dairy farming. There is no common vision on the problem of interspecies transmission of BLV. Therefore, a detailed study of the etiologic relationship between leukemia in cattle and other animal species is relevant. Various laboratory animal models provide insight into the pathogenesis of viral infections. The article presents the research results of two series rabbits’ intravenous infection with bovine leukemia virus (BLV) using the culture antigen FLK-BLV and the blood of rabbits with clinical, hematological and immunological signs of viral tumor growth. Blood from all animals was taken from the ear vein after 14, 21, 30 days, and then monthly for six months: to study the morphological parameters of blood and to determine the titer of antibodies to BLV. Blood serum for the presence of antibodies to BLV was examined using a diagnostic kit for the indication of animals infected with the leukemia virus in an immunodiffusion reaction produced by LLC “SRE Veterinary Medicine”, Kharkiv. It was found that the stage of the BLV provirus in the blood leukogram of infected animals was characterized by pronounced lymphocytosis on the 21st day of the experiment. The highest concentration of antibodies to BLV in the blood serum was found on the 90th day after the administration of the virus-containing material, which disappeared from the blood on the 150–180th day after infection. In experimental rabbits, after five months for thirty days, in the absence of antibodies to leukemia in the blood serum, multiple tumors of a dense consistency began to develop throughout the body. Such clinical signs and changes in the of rabbits’ blood of the experimental group are characteristic of serologically positive cows on the hematological development stage of leukemic process and correlate with the results of domestic and foreign authors. The presence of a large number of lymphoblasts, as well as leukolysis cells, in the histological preparation of lymph nodes, lungs, heart and the accumulation of lymphocytes’ immature forms around the interlobular vessels of the liver, which were found in pathohistological studies of the experimental rabbits’ organs, may indicate the development of the leukemia process on early stage in them. The results obtained indicate the ability of BLV to overcome successfully the interspecies barrier upon parenteral ingestion of heterologous individuals from infected lymphocytes and in the form of a culture antigen.

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Section: таблицяunclassified

Section: обговоренняunclassified

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Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Романишина

Feschenko

Rinyak³

et al. 2020

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“…One of the most pressing diseases in this case is cattle leukemia (EBL) both in the Belgorod region and in most countries of the world, causing a high level of infection in livestock and the associated economic costs of antiepizootic measures. A rather late detection of infection in animals, despite the use of modern diagnostic tests, contributes to the wide spread of leukemia in cattle [1,2]. Modern requirements for recovery from bovine leukemia are in need of early detection of virus-infected animals in the early stages of development of the incubation period.…”

Section: Introductionmentioning

confidence: 99%

Development of approaches to the diagnosis of cattle leukemia in the system of antiepizootic measures in the Belgorod Region

et al. 2021

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The aim of the research is the use of serological and molecular genetic methods for detecting virus-infected cattle leukemia, as well as determining the significance of PCR in identifying BLV infected calves in the system of antiepizootic health measures. The developed technique for early diagnosis of leukemia in cattle made it possible to accelerate the process of recovery of disadvantaged farms in the Belgorod region by increasing the frequency of studies from 6 months to 2-3 months and an increase in the sensitivity of the agar-gel immunodiffusion test. This, in turn, leads to an increase in the sensitivity of the agar-gel immunodiffusion test and makes it possible to detect, on average, from 8.8% to 20.25% more animals infected with the leukemia virus compared to the standard reaction of the agar-gel immunodiffusion test. The additional use of molecular genetic tests for the detection of proviral DNA of the leukemia virus makes it possible to identify at the early stages of the development of the leukemia process, in calves from 15 days of age, the genomic material of bovine leukemia virus, which will also allow in a shorter time to carry out a qualitative improvement of young cattle in dysfunctional farms.

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The role of colostral immunity in the effectiveness of serological detection of BLV

2021

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The purpose of our research was to study the influence of newborn calves colostral antibodies on the efficiency of bovine leukemia virus detection by serological methods. The research was conducted on the basis of agricultural organizations within the territory of the Ural Federal District (Chelyabinsk region). We undertook comprehensive diagnostic studies of calves fed with milk from seropositive and seronegative animals using polymerase chain reaction, ELISA test, and immunodiffusion test. Experimentally it was found that feeding calves with milk from seropositive and seronegative cows led to colostral immunoglobulins persistence for up to 3-3.5 months, in most of the animals, which may be associated with the end of suckling period in calves. At the same time, serological screening of animals during this period is difficult due to the influence of a high concentration of colostral antibodies. The obtained results of laboratory studies indicate the efficiency of PCR diagnostics in detecting a specific locus of the calf leukemia virus in animals at the early stages of the infectious process. PCR diagnostics provides an opportunity to early evaluation of calves with a latent course of BLV, excluding the influence of colostral immunity, thereby reducing the recovery time of enterprises from the leukemia virus by 20%.

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Detection of specific antibodies of classes G and M to bovine leukemia virus in the blood serum

Cited by 9 publications

References 15 publications

Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Development of approaches to the diagnosis of cattle leukemia in the system of antiepizootic measures in the Belgorod Region

The role of colostral immunity in the effectiveness of serological detection of BLV

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