Detection of simian virus 40 DNA sequences in Egyptian patients with different hematological malignancies

Zekri, Abdel‐Rahman N.; Mohamed, Waleed S.; Bahnassy, Abeer A.; Refat, Lobna; Khaled, M Hussein; Shalaby, Sameh; Hafez, Mohamed M.

doi:10.1080/10428190701534408

Cited by 11 publications

(11 citation statements)

References 22 publications

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“…SV40 DNA sequences have been detected in B-lymphocytes from healthy blood donors and immunosuppressed patients (David et al, 2001;Dolei et al, 2000;Martini et al, 1996). B-cell lymphomas arise in some hamsters inoculated with SV40 (Cicala et al, 1992;Diamandopoulos, 1973;Matthews et al, 1987) and human B-cell lymphomas were found in recent investigations to contain SV40 markers (Amara et al, 2007;David et al, 2001;Meneses et al, 2005;Nakatsuka et al, 2003;Shivapurkar et al, 2002;Vilchez et al, 2002b;Zekri et al, 2007). Our findings here suggest that B-cell-rich tonsil tissue might play a role in viral persistence.…”

Section: Discussionsupporting

confidence: 61%

“…The nature of interaction of SV40 with lymphoid tissue is unclear. Bcell lymphomas occur in small-animal models of SV40 infection (Cicala et al, 1992;Diamandopoulos, 1973;Matthews et al, 1987), and SV40 is associated with some B-cell lymphomas in humans (Amara et al, 2007;David et al, 2001;Meneses et al, 2005;Shivapurkar et al, 2002;Vilchez et al, 2002b;Zekri et al, 2007). Therefore, B-cell-rich tissues such as tonsils may represent a site that harbors SV40.…”

Section: Introductionmentioning

confidence: 99%

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Detection of polyomavirus SV40 in tonsils from immunocompetent children

Patel

Vilchez

Killen

et al. 2008

Journal of Clinical Virology

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Background-BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) are nonenveloped DNA viruses, members of the family Polyomaviridae. BK and JC viruses establish persistent infections in humans, and evidence suggests that SV40 can infect humans, as well. Whether persistence occurs in the lymphoid system is unknown.Methods-Paraffin-embedded tonsils from 220 immunocompetent children (mean age 9.3 years) were examined by polymerase chain reaction (PCR) to detect viral DNA of BKV, JCV, SV40, and Epstein-Barr virus (EBV).Results-Polyomavirus-specific DNA sequences were detected in 8.3% (29/351) of specimens collected from 220 children. Twenty-one (9.5%) children had polyomavirus DNA present in at least one tonsil, with sequences identified as SV40 (n=20) and BKV (n=1). Polyomavirus JCV was not detected. Among patients positive for SV40, 8 of 14 (57%) contained viral DNA in both available tonsils. EBV DNA was detected in 99 (28.2%) samples from 67 (30.5%) patients. Eleven samples (3.1%) from 8 (3.6%) children were positive for both polyomavirus and EBV. SV40-positive children were significantly older than the SV40-negative subjects (P < 0.001). T-antigen expression was detected in an SV40 DNA-positive tonsil by immunohistochemistry.Conclusions-These results suggest that SV40 can infect tonsils, that lymphoid tissue may represent a site for polyomavirus persistence, and that immunohistochemistry is not a useful detection assay when there are very few virus-infected cells in a tissue.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Detection of polyomavirus SV40 in tonsils from immunocompetent children

Patel

Vilchez

Killen

et al. 2008

Journal of Clinical Virology

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“…More recently, a study conducted with an innovative analysis, known as RNA sequencing (RNA-seq), identified SV40 mRNAs, in tumor samples from low-grade glioma affected patients (219). Different lympho-proliferative disorders (85, 239, 254, 255), including non-Hodgkin's lymphoma (79, 247–251, 260) have also been associated with SV40 infection. It has been reported that the homologous and autologous implantation of SV40-transformed cells in humans caused the growth of nodules, thus suggesting that this PyV presents oncogenic capacity in humans (261).…”

Section: Association Of Sv40 With Human Tumorsmentioning

confidence: 99%

Association Between Simian Virus 40 and Human Tumors

et al. 2019

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Simian virus 40 (SV40) is a small DNA tumor virus of monkey origin. This polyomavirus was administered to human populations mainly through contaminated polio vaccines, which were produced in naturally infected SV40 monkey cells. Previous molecular biology and recent immunological assays have indicated that SV40 is spreading in human populations, independently from earlier SV40-contaminated vaccines. SV40 DNA sequences have been detected at a higher prevalence in specific human cancer specimens, such as the brain and bone tumors, malignant pleural mesotheliomas, and lymphoproliferative disorders, compared to the corresponding normal tissues/specimens. However, other investigations, which reported negative data, did not confirm an association between SV40 and human tumors. To circumvent the controversies, which have arisen because of these molecular biology studies, immunological researches with newly developed indirect ELISA tests were carried out in serum samples from patients affected by the same kind of tumors as mentioned above. These innovative indirect ELISAs employ synthetic peptides as mimotopes/specific SV40 antigens. SV40 mimotopes do not cross-react with the homologous human polyomaviruses, BKPyV, and JCPyV. Immunological data obtained from indirect ELISAs, using SV40 mimotopes, employed to analyze serum samples from oncological patients, have indicated that these sera had a higher prevalence of antibodies against SV40 compared to healthy subjects. The main data on (i) the biology and genetics of SV40; (ii) the epidemiology of SV40 in the general population, (iii) the mechanisms of SV40 transformation; (iv) the putative role of SV40 in the onset/progression of specific human tumors, and (v) its association with other human diseases are reported in this review.

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“…Moreover, few polymerase chain reaction (PCR) studies have shown the presence of SV40 in lymphomas with contradicting results. Two Egyptian studies using multiplex nested PCR have shown that SV40 DNA sequences were found in 53.8% of NHL patients in both series [ 23 , 24 ]. Other studies found 13, 10, 14, 42, and 43% incidence of SV40 in NHL, respectively [ 17 , 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

The Prognostic Role and Relationship between E2F1 and SV40 in Diffuse Large B-Cell Lymphoma of Egyptian Patients

Samaka

Aiad

Kandil

et al. 2015

Analytical Cellular Pathology

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Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphomas worldwide. The pathogenesis of lymphomas is not yet well understood. SV40 induces malignant transformation by the large T-antigen (L-TAG) and promotes transformation by binding and inactivating p53 and pRb. L-TAG can bind pRb promoting the activation of the E2F1 transcription factor, thus inducing the expression of genes required for the entry to the S phase and leading to cell transformation. This immunohistochemical study was conducted to assess the prognostic role and relationship of SV40 L-TAG and E2F1 in diffuse large B-cell lymphoma (DLBCL) of Egyptian patients. This retrospective study was conducted on 105 tissue specimens including 20 follicular hyperplasia and 85 DLBCL cases. SV40 L-TAG was identified in 3/85 (4%) of DLBCL. High Ki-67 labeling index (Ki-67 LI) and apoptotic count were associated with high E2F1 expression (p<0.001 for all). No significant association was reached between E2F1 and SV40. E2F1 expression proved to be the most and first independent prognostic factor on overall survival of DLBCL patients (HR = 5.79, 95% CI = 2.3–14.6, and p<0.001). Upregulation of E2F1 has been implicated in oncogenesis, prognosis, and prediction of therapeutic response but is not seemingly to have a relationship with the accused SV40.

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Detection of simian virus 40 DNA sequences in Egyptian patients with different hematological malignancies

Cited by 11 publications

References 22 publications

Detection of polyomavirus SV40 in tonsils from immunocompetent children

Detection of polyomavirus SV40 in tonsils from immunocompetent children

Association Between Simian Virus 40 and Human Tumors

The Prognostic Role and Relationship between E2F1 and SV40 in Diffuse Large B-Cell Lymphoma of Egyptian Patients

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