Detection of Rickettsia felis in ectoparasites collected from domestic animals

Oliveira, Jéssica Cardoso Pessoa de; Reckziegel, Guilherme Henrique; Ramos, Carlos Alberto do Nascimento; Giannelli, Alessio; Alves, Lêucio Câmara; Carvalho, Gílcia Aparecida de; Ramos, Rafael Antonio Nascimento

doi:10.1007/s10493-020-00505-2

Cited by 7 publications

(4 citation statements)

References 41 publications

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“…These tick species include Rhipicephalus sanguineus in Brazil, Spain, Chile, China, and the Philippines [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]; Rhipicephalus bursa in Turkey [ 22 ]; Rhipicephalus turanicus in Italy [ 23 ]; Haemaphysalis flava, Haemaphysalis kitasatoe, and Ixodes ovatus in Japan [ 24 ]; Haemaphysalis sulcata in Croatia [ 25 ]; Haemaphysalis leporispalustris in the United States [ 26 ]; Haemaphysalis sp. and Rhipicephalus microplus in Malaysia [ 27 ]; Heamaphysalis bancrofti in Australia [ 28 ]; Amblyomma maculatum in the United States [ 29 ]; Amblyomma cajennense , Amblyomma humerale, Amblyomma ovale , and Amblyomma sculptum in Brazil [ 14 , 30 , 31 , 32 ]; Ixodes granulatus in Taiwan [ 33 ]; Ixodes hexagonus in Italy [ 34 ]; Ixodes ricinus in Germany, France, Spain, Romania, and Serbia [ 35 , 36 , 37 , 38 , 39 , 40 ]; Dermacentor nitens in Brazil and Cuba [ 21 , 41 ]; and Dermacentor variabilis and Carios capensis in the United States [ 42 , 43 ] ( Table S1 ). The previous reports of the presence of R. felis in hard and soft ticks are not necessarily related to a vectorial role, and to date, the role of ticks in the ecology and transmission of R. felis has not been clarified.…”

Section: Introductionmentioning

confidence: 99%

Culture Isolate of Rickettsia felis from a Tick

Danchenko

Benada

Škultéty

et al. 2022

IJERPH

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Although the cat flea, Ctenocephalides felis, has been identified as the primary vector of Rickettsia felis, additional flea, tick, mite, and louse species have also been associated with this bacterium by molecular means; however, the role of these arthropods in the transmission of R. felis has not been clarified. Here, we succeeded in culture isolation of R. felis from a host-seeking castor bean tick, Ixodes ricinus, the most common tick in Slovakia. The bacterial isolation was performed on XTC-2 cells at 28 °C using the shell-vial technique. An evaluation of the growth properties was performed for both the XTC-2 and Vero cell lines. We observed R. felis in the infected host cells microscopically by Gimenez staining and immunofluorescence assay. The R. felis isolate was purified by gradient ultracentrifugation and visualized by electron microscopy. Fragments of the genes gltA, ompA, ompB, htrA, rpoB, sca4, rffE, and rrs were amplified and compared with the corresponding sequences of the type strain URRWXCal2 and other R. felis culture -isolated strains. We did not detect any nucleotide polymorphisms; however, plasmid pRFδ, characteristic of the standard strain, was absent in our isolate. Herein, we describe the first successful isolation and characterization of a tick-derived R. felis strain “Danube”, obtained from an I. ricinus nymph.

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Section: Introductionmentioning

confidence: 99%

Culture Isolate of Rickettsia felis from a Tick

Danchenko

Benada

Škultéty

et al. 2022

IJERPH

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“…Rickettsia felis, a zoonotic rickettsia belonging to the SFG, previously described infecting human patients from Kenya (Richards et al 2010) and Serbia (Banović et al 2021), was molecularly detected for the first time in the dogs herein assessed. This pathogen was also detected in fleas (Ctenocephalides felis) and ticks (Dermacentor nitens) collected from dogs and horses from the same study area (Oliveira et al 2020). Rickettsia felis is widespread in Brazil, and its distribution overlaps that of C. felis, its main biological vector (Horta et al 2014).…”

Section: Discussionmentioning

confidence: 84%

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

et al. 2022

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Canine vector-borne pathogens (CVBPs) comprise a group of disease agents mainly transmitted by ticks, fleas, mosquitoes and sand flies. In this study, we assessed the presence of CVBPs in an Afro-descendent community (Quilombola) of northeastern, Brazil. Dog blood samples (n = 201) were collected and analyzed by rapid test for the detection of antibodies against Leishmania spp., Anaplasma spp., Ehrlichia spp. and Borrelia burgdorferi sensu lato (s.l.), and antigens of Dirofilaria immitis. In addition, polymerase chain reactions were performed for Anaplasmataceae, Babesia spp., Hepatozoon spp., Rickettsia spp. and B. burgdorferi s.l. Overall, 66.7% of the dogs scored positive to at least one pathogen at serological and/or molecular methods. Antibodies against Ehrlichia spp. were the most frequently detected (57.2%; n = 115/201), followed by Anaplasma spp. (8.5%; n = 17/201), Leishmania spp. (8.5%; n = 17/201) and B. burgdorferi s.l. (0.5%; n = 1/201). For D. immitis, 11 out of 201 (5.5%) animals scored positive. At the molecular analysis, 10.4% (n = 21/201) of the samples scored positive for Babesia spp./Hepatozoon spp., followed by Anaplasmataceae (5.0%; n = 10/201) and Rickettsia spp. (3.0%; n = 6/201). All samples were negative for B. burgdorferi s.l. Our data demonstrated the presence of CVBPs in the studied population, with a high seropositivity for Ehrlichia spp. In addition, considering the detection of zoonotic pathogens in dogs and their relationship with people from Quilombola communities, effective control strategies are advocated for minimizing the risk of infection in this socially vulnerable human population and their pets.

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“…Opossums harbor a great diversity of flea species (Horta et al 2007;Pinto et al 2009;Urdapilleta et al 2019;Bezerra-Santos et al 2020b;Canto-Osorio et al 2020), with some of them being regarded as important vectors of zoonotic pathogens (e.g., Ctenocephalides felis felis, Xenopsylla cheopis, Pulex irritans and Pulex simulans), while others (e.g., Adoratopsylla antiquorum, Polygenis occidentalis and Cediopsylla simplex) presenting unknown vector role as studies on their ecology and epidemiology are scanty (Table 2). Among the pathogens transmitted by fleas reported on Didelphis spp., R. typhi, R. felis and Y. pestis are of major public health concern (Azad et al 1997;Demeure et al 2019;Oliveira et al 2020). Rickettsia typhi is the etiological agent of the murine typhus, a worldwide distributed zoonosis with a life cycle involving rodents (Rattus rattus and Rattus norvegicus) as vertebrate hosts, and fleas (X. cheopis) as vectors (Peniche-Lara et al 2015).…”

Section: Fleas and Flea-borne Pathogensmentioning

confidence: 99%

Didelphis spp. opossums and their parasites in the Americas: A One Health perspective

et al. 2021

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Medium sized opossums (Didelphis spp.) are among the most fascinating mammals of the Americas, playing important ecological roles (e.g., dispersal of seeds and control of insect populations) in the environment they inhabit. Nevertheless, as synanthropic animals, they are well adapted to human dwellings, occupying shelters within the cities, peripheral areas, and rural settings. These marsupials can harbor numerous pathogens, which may affect people, pets, and livestock. Among those, some protozoa (e.g., Leishmania infantum, Trypanosoma cruzi, Toxoplasma gondii), helminths (e.g., Ancylostoma caninum, Trichinella spiralis, Alaria marcianae, Paragonimus spp.) and arthropods (e.g., ticks, fleas) present substantial public health and veterinary importance, due to their capacity to cause disease in humans, domestic animals, and wildlife. Here, we reviewed the role played by opossums on the spreading of zoonotic parasites, vectors, and vector-borne pathogens, highlighting the risks of pathogens transmission due to the direct and indirect interaction of humans and domestic animals with Didelphis spp. in the Americas.

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Detection of Rickettsia felis in ectoparasites collected from domestic animals

Cited by 7 publications

References 41 publications

Culture Isolate of Rickettsia felis from a Tick

Culture Isolate of Rickettsia felis from a Tick

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

Didelphis spp. opossums and their parasites in the Americas: A One Health perspective

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